Protective Ability of Ethanol Extracts ofHypericum ScabrumL. andHypericum RetusumAucher Against the Protein Oxidation and DNA Damage

Abstract

Inhibition of protein oxidation and DNA damage by reactive oxygen species (ROS) would confer benefit to living organisms exposed to oxidative stress, because oxidized proteins are associated with many diseases and can propagate ROS-induced damage. In this study, the ability of the ethanol extracts of Hypericum retusum Aucher (HR) and Hypericum scabrum L (HSm) to protect bovine serum albumin (BSA) from oxidation and DNA damage by hydroxyl radical derived from the Fenton system (Fe 3+ /H 2 O 2 /ascorbic acid) were measured. The ethanol extracts of HR and HSm at different concentrations (50-1000 µg/mL) efficiently prevented protein oxidation induced by hydroxyl radicals as assayed by protein oxidation markers, including protein carbonyl formation (PCO) and polyacrylamide gel electrophoresis. The effect of ethanol extracts of HR and HSm on DNA cleavage induced by UV-photholysis of H 2 O 2 using pBluescript M13+ plasmid DNA were also investigated. These extracts significantly inhibited DNA damage induced by ROS. Therefore, Hypericum retusum, and Hypericum scabrum extracts may be useful in the food industry as effective synthetic antioxidants.

Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts ofRumex crispus

“…ROS are involved in the normal physiology of living organisms. They act as a messenger for signal transduction and also affect gene expression and are also actively involved in the pathogenesis of diseases such diabetes, cancer and neurodegenerative diseases [18]. Significant protective effect of cell proteins (BSA) by RERC as shown in our studies further confirmed that methanolic RERC's are highly anti-oxidatant which protects proteins from ROS attack.…”

“…The effects of RERC on protein oxidation were carried out according the method described by Kızıl et al [15]. Interactions of the samples were done with BSA protein.…”

Optimisation of organic solvent-free polyphenol extraction from Hypericum triquetrifolium Turra using Box–Behnken experimental design and kinetics

“…This assumption is in accordance with previous examinations on various Hypericum species, where it was shown that extracts with higher polyphenolic load also exhibited stronger antioxidant effects [24,25]. Similar results were reported for several Hypericum taxa [26][27][28]. Therefore, the differences in the P R values between the two extracts are rather attributed to differences in the total polyphenol concentration and the relative amounts of the various polyphenols.…”

Assessment of the Antioxidant Activity of Silybum marianum Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

“…Many publications show the inhibitory eff ects of plant extracts or their components against protein oxidation and DNA damage caused by reactive oxygen species (11)(12)(13). To our knowledge, this is the fi rst study that has investigated the inhibition ability of DNA damage, protein oxidation and lipid peroxidation by ethanol extract of S. marianum seeds in vitro.…”