Protective activity of ischemic preconditioning on rat testicular ischemia: Effects of Y-27632 and 5-hydroxydecanoic acid

Gözen, Ahmet; Demiryürek, Şeniz; Taşkın, Abdullah; Çıralık, Harun; Bilinc, Hasan; Kara, Sevgül; Aydın, Abdullah; Aksoy, Nurten; Ceylan, Hasan Hüseyin

doi:10.1016/j.jpedsurg.2012.10.074

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…The first evaluated seminiferous tubule was randomly selected, and the section was shifted clockwise to evaluate the others. Histopathologic scoring was assessed by the modified Johnson scoring method (Table 5) 46 .…”

Section: Methodsmentioning

confidence: 99%

Reproductive effects of subchronic exposure to acetamiprid in male rats

Arıcan

Kayalı

Karaca

et al. 2020

Sci Rep

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Acetamiprid, a selective agonist of nicotinic acetylcholine recetors, is one of the most widely used neonicotinoids. There is limited data about toxicity of acetamiprid on male reproductive system. Therefore, the study aimed to investigate the reproductive toxic potential of acetamiprid in male rats orally treated with acetamiprid with low (12.5 mg/kg) medium (25 mg/kg) or high dose (35 mg/kg) for 90 days. According to our results, sperm concentration and plasma testosterone levels decreased in dose dependent manner. Gonadotropin-releasing hormone (GnRH), follicle-stimulating hormeone (FSH), luteinizing hormone (LH) levels increased at low and medium dose groups and acetamiprid caused lipid peroxidation and glutathione (GSH) depletion in the testes. Histologic examinations revealed that acetamiprid induced apoptosis in medium and high dose groups and proliferation index dramatically decreased in high dose group. In conclusion, acetamiprid caused toxicity on male reproductive system in the high dose. The mechanism of the toxic effect may be associated with oxidative stress, hormonal disruptions and apoptosis. Neonicotinoids are new class of insecticides that act as selective nicotinic acetylcholine receptor (nAChR) agonist selectively in central nervous system of insects 1. Acetamiprid, one of the neonicotinoid insecticides, is commonly used for agricultural and domestic purposes against a large variety of insects 2,3. Acetamiprid has been reported to accumulate in plants and contaminate water and this can pose a potential risk for human health 4,5. Acetamiprid is absorbed easily after oral administration, and it is determined at the highest concentration in the liver, kidney, adrenal and thyroid glands 6. Some researchers showed that acetamiprid caused toxic effects on several organ systems, including the nervous, respiratory, and immune systems in the experimental models 7-9. Furthermore, it has been reported acute poisoning cases after ingestion of acetamiprid in humans 10,11. Acetamiprid has also been reported to induce reproductive toxicity in different species 12,13. The cross-sectional epidemiological study which was conducted in Kavar, (Iran) showed acetamiprid reduced the number of sperm in farmers who exposed to acetamiprid 14. As the use of acetamiprid is increasing, it is very important to identify the toxicity of acetamiprid. Additionally, acetamiprid can be used in combinations with other insecticide because of that, toxic effects and doses of acetamiprid are needed to elucidate well by chronic and subchronic toxicity studies. Acetamiprid has been shown toxic effects on many organs and systems. However, there is no satisfied information on the toxicity potential of acetamiprid on male reproductive system. In this study, it was aimed to examine the effects of acetamiprid on reproductive function of male rats in terms of oxidative stress, apoptosis, hormonal disruptions and histopathological changes. Results Effect of acetamiprid on body and testicular weights. Liver steatosis and slowness of the movem...

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Section: Methodsmentioning

confidence: 99%

Reproductive effects of subchronic exposure to acetamiprid in male rats

Arıcan

Kayalı

Karaca

et al. 2020

Sci Rep

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show abstract

“…The first seminiferous tubules were selected randomly and the others were taken by sliding the section towards the clockwise. Histopathological scoring was evaluated by the modified Johnson scoring method as shown in Table 1 [17].…”

Section: Histological Analysismentioning

confidence: 99%

Apocynin attenuates testicular ischemia–reperfusion injury in rats

Sener

Yüksel

Ozyilmaz-Yay

et al. 2015

Journal of Pediatric Surgery

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“…Periodic acid-Schiff (PAS) reaction was applied to reveal the structure of the basal membranes of seminiferous tubules. Seminiferous tubules in H&E-stained testicular sections were scored on the basis of modified Johnsen’s histopathological scoring parameters [ 30 , 31 ]. In addition, the epithelial thicknesses of 100 seminiferous tubules were measured in all sections using Image J (Image J software, National Institutes of Health) program.…”

Section: Methodsmentioning

confidence: 99%

Apocynin Ameliorates Monosodium Glutamate Induced Testis Damage by Impaired Blood-Testis Barrier and Oxidative Stress Parameters

Elmas

Algilani

Sahin

et al. 2023

Life

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Background: the aim of this study was to investigate the effects of apocynin (APO) on hormone levels, the blood-testis barrier, and oxidative biomarkers in monosodium glutamate (MSG) induced testicular degeneration. Methods: Sprague Dawley male rats (150–200 g; n = 32) were randomly distributed into four groups: control, APO, MSG, and MSG + APO. MSG and MSG + APO groups were administered MSG (120 mg/kg) for 28 days. Moreover, the APO and MSG + APO groups received APO (25 mg/kg) during the last five days of the experiment. All administrations were via oral gavage. Finally, biochemical analyses were performed based on the determination of testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD), as well as light and transmission electron microscopic examinations, assessment of sperm parameters, ZO-1, occludin, NOX-2, and TUNEL immunohistochemistry were evaluated. Results: MSG increased both the oxidative stress level and apoptosis, decreased cell proliferation, and caused degeneration in testis morphology including in the blood-testis barrier. Administration of apocynin reversed all the deteriorated morphological and biochemical parameters in the MSG + APO group. Conclusions: apocynin is considered to prevent testicular degeneration by maintaining the integrity of the blood-testis barrier with balanced hormone and oxidant/antioxidant levels.

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Protective activity of ischemic preconditioning on rat testicular ischemia: Effects of Y-27632 and 5-hydroxydecanoic acid

Cited by 20 publications

References 39 publications

Reproductive effects of subchronic exposure to acetamiprid in male rats

Reproductive effects of subchronic exposure to acetamiprid in male rats

Apocynin attenuates testicular ischemia–reperfusion injury in rats

Apocynin Ameliorates Monosodium Glutamate Induced Testis Damage by Impaired Blood-Testis Barrier and Oxidative Stress Parameters

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