Protective effect of adiponectin on paraquat-induced pulmonary fibrosis in mice

Abstract

Pulmonary fibrosis (PF) is the most com mon complication of paraquat (PQ) toxicity, which lacks an effective treatment. This study aimed to inve stigate whether exogenous administration of globular adiponectin (APN) isoform provided protection ag ainst PQinduced PF, and examined the possible me chanisms underlying these effects. Eighty BALB/C mice were randomly divided into control, PQ, low dose APN, and highdose APN groups. Human lung WI-38 fibroblasts were similarly divided into control, PQ, and APN groups and examined at 24 h, 48 h, and 72 h after PQ exposure. Hematoxylin and eosin (HE) and Masson trichrome staining were used to compare the histopathologic changes in the mouse lung tissues. Western blot and realtime quantitativePCR (RT PCR) were used to measure the protein and mRNA expression of transforming growth factor β1 (TGF-β1) in mice lung tissues and α-SMA, type III collagen, and NFκB p65 in lung fibroblasts. Dihydroethidium (DHE) was used to detect intracellular superoxide anion (O2 -) in fibroblasts. APN administration significantly ameliorated PQmediated fibrosis histologically and reduced the protein and mRNA expression levels of TGF-β1 in mouse lung tissues in a dosedependent manner (P<0.05). When fibroblasts were pretreated with APN, the expression of α-SMA and NF-κB p65 were downregulated, and O2 -decreased. Expression of the anti-inflammatory factor IL-1Ra was upregulated following PQ exposure (P<0.05). This study revealed the beneficial effects of APN against PQinduced PF, which may have occurred through suppression of NF κB dependent inflammatory and TGF-β1 mediated fibrotic events.

Bleomycin Inhibits Proliferation via Schlafen-Mediated Cell Cycle Arrest in Mouse Alveolar Epithelial Cells

“…Idiopathic pulmonary fibrosis (IPF) is the irreversible form of the idiopathic interstitial pneumonia that has symptoms of chronic and progressive dyspnea by unknown causes 1 2 . IPF is etiologically characterized with alveolar epithelial cell injury, fibroblast/myofibroblast proliferation, and excessive extracellular matrix (ECM) deposition leading to the destruction of the alveolar structure.…”

Chlorin e6-Mediated Photodynamic Therapy Suppresses P. acnes-Induced Inflammatory Response via NFκB and MAPKs Signaling Pathway

“…Three MAPKs; ERK, p38 and JNK, are known to be activated by several external stimulants including ROS and microbial infection [35,36]. MAPKs play an important role in the transcriptional regulation of iNOS, COX-2 and inflammatory cytokines via activation of the transcription factor NFκB in various cells [37–39]. In this investigation, the phosphorylated p38, ERK and JNK significantly increased in heat-killed P .…”