Protective Effect of Broccoli and Ferulic Acid on Imidacloprid-Induced Hepatotoxicity in Rat

Abstract

Imidacloprid may induce oxidative stress leading to generate free radicals and alternate oxygen free radical scavenging enzyme system. This study aims to investigate the hepatoprotective effect of broccoli water extract and ferulic acid on imidacloprid induced oxidative stress and DNA damage in male albino rats. Rats were co-treated with broccoli water extract (200 mg/kg) or ferulic acid (20 mg/kg) with imidacloprid (80 mg/kg) orally for 28 days.The results revealed that imidacloprid induced high serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). However, administration of broccoli and ferulic acid reduced these parameters. Broccoli and ferulic acid significantly (P<0.05) attenuated the imidacloprid-induced increases in lipid peroxidation (LPO), tumor necroses factor α (TNF-α) and nitric oxide (NO) contents and meyloperoxidase (MPO), glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PD) activities. Imidacloprid decreased reduced glutathione (GSH) while co-treatment with broccoli and ferulic acid significantly (P<0.05) improved the level of GSH. DNA damage as assessed by comet assay was increased in imidacloprid-treated group. However, DNA damage was decreased in M ahgoub M . A hm ed1 and Saw san A . N asr ‫ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ‬ ‫ــــــــــــــــــــــــــــــــــ‬ ‫ـــــــــــــــــــــــ‬ ‫ـــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ‬ 2 broccoli and ferulic acid treated groups. The possible mechanism of broccoli and ferulic acid extract on imidacloprid might be due to decreasing oxidative stress (LPO, NO and DNA damage) and increasing GSH content. Thus, broccoli and ferulic acid was suggested to protected rat's liver against imidacloprid-induced oxidative stress and DNA damage in liver. : Unifying mechanism for eye toxicity, Electron transfer, reactive oxygen species, antioxidant benefits, cell Signaling and cell Membranes. Cell Membrane Free Radical Research 2: 56-69. Lowry O.H., Roseborough N.J., Farr A.L., and Randall R.L. (1951): Protein measurement with phenol reagent. Journal of Biological Chemistry. 193 (1): 265-275. Marks P.A. (1961): Enzymes of the pentose phosphate pathway. Methods Med. Res. 9: 24-35. Moreno D.A., Carvaja M., López-Berenguer C. and García-Viguera N. (2006): Chemical and biological characterisation of nutraceutical compounds of broccoli. J Pharm Biomed Anal. 41: 1508-1522. Olsen R.L. and Little C. (1983): Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood. Biochem. J. 209 (3): 781-787. Ou S. and Kwok K. C. (2004): Ferulic acid: pharmaceutical functions, preparation and applications in foods. J Sci. Food and Agric. 84 (11): 1261-1269. Podsedek A. (2007): Natural antioxidants and antioxidant capacity of Brassica vegetables: a review. LWT 40: 1-11. Rukkumani R., Aruna K., Suresh Varma P., Padmanabhan L. and Me...

Teratogenic effects of imidacloprid in rats: mechanistic role of oxidative stress

“…Our findings are consistent with previous studies that confirmed the ability of IMI to induce oxidative damages in various tissues (Duzguner and Erdogan, 2012;Ahmed and Nasr, 2015;Chakroun et al, 2017;El-Sabbagh et al, 2018). Also, several studies suggested the role of oxidative stress in the mechanisms of adverse imidacloprid effects on male and female reproduction (Kapoor et al, 2011;Bal et al, 2012;Lonare et al, 2016) The recorded increase in MDA and H2O2 levels in serum of dams concomitant together with the decrease in GSH content and CAT activity could be attributed to excessive ROS generation induced by maternal exposure to IMI (Kapoor et al, 2010) that may be considered as one of the leading reasons to oxidative stress and lipid peroxidation and hence, oxidative tissue damages of the pregnant dams that may affect their offsprings.…”

Colibri® insecticide induces male reproductive toxicity: alleviating effects of Lannea acida (Anacardiaceae) in rats

“…Toxic potentials of technical IMI, a neonicotinoid insecticide against mammal’s reproductive function, have been largely documented [5, 6, 25]. Some authors demonstrated that medicinal plants (broccoli) or plant-derived compounds (Ferulic acid or Curcumin ) could be effective in preventing IMI toxicity [12, 13]. Contrary to previous reports which focused on the pure compound, the current study evaluated the reproductive toxicity of colibri®, a commercial formulation made of 3% IMI and the curative potentials of L. acida, an aphrodisiac medicinal plant, in adult male rats.…”

“…As oxidative stress tends to be the common feature of many xenobiotic against human systems, extensive research activity has been performed during recent years to find effective and safety solutions, particularly natural compounds with antioxidant properties. In fact, the preventive effect of Broccoli and Curcumin against neurotoxicity and reproductive toxicity respectively of IMI has been reported in rat [12, 13]. However, less is known about the curative potentials of medicinal plants on the reproductive impairments due to IMI.…”

Capoeta capoeta’da Lambda Cyhalothrin’in Biyokimyasal ve Moleküler Karakterizasyonu

“…Liu et al (2015) Japon balığı üzerine uygulamış oldukları organofosforlu böcek ilacı Triazophosun etkilerine bakmış ve sonuç olarak GSH, SOD, CAT ve laktat dehidrogenaz (LDH) aktivitelerinin azaldığını açıklamışlardır (Liu et al, 2015). Ahmet ve Nasr (2015) erkek albino sıçanlar üzerinde uygulamış oldukları brokoli su ekstraktı, ferulik asit ve imidaklopridinin oksidatif stres üzerine sonuçlarını araştırmışlar ve imidaklopridinin, LPO içeriklerini artırdığını, Brokoli ve ferulik asit LPO seviyesini azalttığını, imidaklopridin GSH'yı düşürmüş, brokoli ve ferulik asit birlikte kullanıldığı zaman ise GSH düzeyini iyileştirdiğini açıklamışlardır (Ahmed and Nasr, 2015).…”

“…Gowri et al (2013) tatlı su balığı Cyprinus carpio üzerine sentetik pretroitlerden sipermetrini uygulamışlar ve 7 gün içerisinde bu kimyasala maruz kalan balıkların DNA ve RNA içeriklerinde değişiklik olmadığı yalnız 14 ve 21 gün süreyle bekletilen balıkların ise DNA ve RNA içeriğinin beyin, solungaçlar ve karaciğerde önemli ölçüde düştüğünü vurgulamışlardır (Gowri, Govindassamy, and Ramalingam, 2013). Ahmet ve Nasr (2015) erkek albino sıçanlar üzerinde uygulamış oldukları brokoli su ekstraktı, ferulik asit ve imidaklopridinin 8-OHdG seviyesini artırdığını ve brokoli ve ferulik asitin kullanıldığı seviyelerde 8-OHdG azaldığını açıklamışlardır (Ahmed and Nasr, 2015). Calviello et al (2006) sıçanlar üzerinde fungusit olan Mancozebi uygulamışlar ve 8-OHdG DNA oksidasyon ve ROS seviyelerinde düşük bir artışın olduğunu ifade etmişlerdir (Calviello et al, 2006).…”