Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Naseer, Zahid; Ahmad, Ejaz; Aksoy, Melih; Küçük, Niyazi; Serin, İlker; Ceylan, Ahmet; Boyacıoğlu, Murat; Kum, Cavit

doi:10.1016/j.cryobiol.2015.06.007

Cited by 25 publications

(13 citation statements)

References 47 publications

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“…There was no statistically significant difference among the groups for oxidative parameters (LPO, AOP and tGSH ) (P>0.05). These findings of lipid peroxidation level and antioxidant capacity were the same with other authors findings for ram semen (14,25). However, Lopez-Revelta et al (19) reported that cholesterol modification in the cell membrane protects antioxidative enzymes and prevents spreading to radio oxidative species (ROS).…”

Section: Discussionsupporting

confidence: 80%

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Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

̇İnanç¹,

Uysal²,

Ata³

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The experiment was conducted to determine the in vitro results of 7-dehydrocholesterol loaded cyclodextrin compound (7-DCLC) addition to tris extenders for freezing ram semen. Three Akkaraman rams, local species in Turkey, were used. Semen samples were extended with tris containing 0 mg (control), 1.5 mg, 2.5 mg and 3.5 mg/120x10 6 7-DCLC and they were equilibrated to 4°C and frozen in mini straws then stored in liquid nitrogen. Straws were thawed at 37°C for 30 s in water bath for spermatological examination. Statistically significant differences were observed between 7-DCLC and control groups in terms of acrosome integrity, CASA motility, HOS test and viability after thawing (P <0.001). In all these parameters, control group had the lowest percentages. There was no statistically significant difference between groups in terms of DNA fragmentation, abnormal spermatozoa ratio, mitochondrial activation, lipid peroxidation and apoptosis level (P> 0.05). In conclusion, it can be said that adding 7-DCLC to Akkaraman ram semen improved cell membrane cryosurvival. Besides, 7-DCLC can be used in tris extender instead of cholesterol loaded cyclodextrin (CLC) compounds for sperm cryopreservation.

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Section: Discussionsupporting

confidence: 80%

“…During the last twenty years, authors have been working about sperm membrane with CLC, aiming to protect sperm from cold injury in different animals (25). Cholesterol is a necessary sterol for cell proliferation and viability.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

̇İnanç¹,

Uysal²,

Ata³

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…These authors verified that cyclodextrin at 3 mg concentration was effective in protecting the sperm against the deleterious effects of H 2 O 2 . They obtained superior plasma membrane motility, viability, and integrity of the CLC-treated samples compared to the control group [19].…”

Section: Discussionmentioning

confidence: 96%

Action of Cholesterol-Loaded Cyclodextrin on Viability of Ram Semen

Silva

Almada

Lima

et al. 2019

Acta Scientiae. Vet.

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Background: Studies report that cyclodextrins have the property of carrying cholesterol to the membrane, but in some cases can also remove this cholesterol from the plasma membrane. The mechanism of action of CLC is not well understood, however, it seems to involve sperm protection during the freezing and thawing process. Studies show that its use enhancing increased osmotic tolerance and reduced premature sperm capacitation reaction. In this sense, studies report that cyclodextrins have the property of carrying cholesterol to the membrane, but in some cases can also remove this cholesterol from the plasma membrane. Improvements were reported in the sperm parameters of buffaloes, bulls, stallions and sheep. Ram naturally present less lipids in their membrane, on average 27%, while bulls have 31%, rabbits 62%, and humans 50%. The aim of the present study was to evaluate the use of cholesterol-loaded cyclodextrin (CLC), a commercial diluent, in the kinetics and viability of frozen and thawed ram spermatozoa. Materials, Methods & Results:Five ejaculates, from five rams of Dorper breed were collected and divided into three groups: control, 1 mg CLC and 2 mg CLC. Semen was diluted in different concentrations of CLC (0, 1, and 2 mg/120×10 6 spermatozoa), and incubated at room temperature (21°C) for 10 min. Samples were conditioned in 0.5 mL straws and incubated at 5°C for 4 h, exposed to LN 2 vapor for 10 min and storing a cryogenic container. The parameters as spermatic kinetics, plasma membrane, acrosomal membrane (MPAI, %), and intracellular levels of superoxide anion (O 2-) were evaluated. Sperm progressive motility (PM), rapid spermatozoa percentage (RAP), linearity (LIN, %), average path velocity (VAP, μm/s) and MPAI (%) were more satisfactory with the use of 1 mg compared to 2 mg (P < 0.05). In addition, 1 mg CLC showed decreased levels of superoxide anion formation (O 2-), a free radical detrimental to spermatozoa (P < 0.05). The use of 2 mg of CLC reduce VAP (P < 0.05) and did not have any beneficial effect on the evaluated parameters. Discussion: Authors did not observe improvement in the parameters of progressive motility when using 1 mg of CLC in goat semen and 2 mg in bull semen with the slow freezing protocol. This differs from our work, as we found that 1 mg of CLC improved the PM parameters, but not at the concentration of 2 mg CLC. Additionally, authors verified that cyclodextrin at 3 mg concentration was effective in protecting the sperm against the deleterious effects of H 2 O 2 . They obtained superior plasma membrane motility, viability, and integrity of the CLC-treated samples compared to the control group. The superoxide anion (O 2-) is a free radical formed from molecular oxygen by the addition of an electron. It is generated spontaneously, mainly in the membrane of the mitochondria, by the respiratory chain and by flavoenzimes, lipoxygenases, and cicloxygenases. In our study, we found a difference between the study group with 1 mg CLC and the control group. Thus, we suggest that CLC may have a ...

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“…In addition, CD-CHL-Vit E was the most treatment that decreased significantly membrane lipid peroxidation. This is probably due to the effects of both Vitamin E, as antioxidant and membrane stabilizer (Urano et al, 1987(Urano et al, , 1988 and cholesterol as a hindrance to ROS propagation (López-Revuelta et al, 2007) and protector against peroxide radical (Naseer et al, 2015).…”

Section: Discussionmentioning

confidence: 98%

Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen

Benhenia

Lamara

Fatmi

et al. 2016

Small Ruminant Research

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Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Cited by 25 publications

References 47 publications

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

Cryopreservation and evaluation of Akkaraman ram semen with 7-dehydrocholesterol

Action of Cholesterol-Loaded Cyclodextrin on Viability of Ram Semen

Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen

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