Protective Effect of Circular RNA (CircRNA) Ddx17 in Ovalbumin (OVA)-Induced Allergic Rhinitis (AR) Mice

Abstract

Background:CircRNAs are involved in multiple biological processes, especially when they act as sponges of miRNA. Thus, the present study investigated the effect of circDdx17 on allergic rhinitis (AR) in an animal model, and determined the miRNA that was involved in this effect. Material/Methods:The AR model was created by repetitive stimulation of ovalbumin (OVA). The levels of mRNAs in plasma were determined by qPCR. CircDdx17 stability was assessed using RNase R. The interaction between circDdx17 and miR-17-5p was predicted by bioinformatics and confirmed by dual luciferase assay. Moreover, the frequencies of rubbing and sneezing and pathological changes were recorded, and OVA-specific IgE, tumor necrosis factor (TNF)-a, interleukin (IL)-4, and IL-5 levels were detected by ELISA. Results:Levels of circDdx17 were decreased in OVA-induced AR mice, and miR-17-5p interacted with circDdx17 in spleen cells derived from mice. Moreover, circDdx17 overexpression reduced the expression of miR-17-5p, OVA-specific IgE, TNF-a, IL-4, and IL-5, as well as the frequencies of rubbing and sneezing, and alleviated pathological changes in OVA-induced AR mice. Conclusions:CircDdx17 appears to have a protective effect on mice in the progression of AR. Specifically, overexpression of circDdx17 inhibited the expression of miR-17-5p and alleviated the condition of AR. Therefore, circDdx17 appears to be a good candidate for use in prevention of AR. However, the detailed mechanism underlying the circDdx17/miR-17-5p regulatory pathway requires further study.

Bioinformatics analysis of mRNA profiles and identification of microRNA-mRNA network in CD4+ T cells in seasonal allergic rhinitis

“…44 Overexpression of circDdx17 inhibited the expression of miR-17-5p and alleviated the allergic condition in an AR animal model. 45 However, the contribution of circRNAs in AR remains largely unknown. The six most promising candidate circRNAs screened out in the current study might thus lead to further investigations of their roles in the pathogenesis of SAR.…”

CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis

“…In addition, circRNAs are associated with the immune response and inflammatory response in humans [8]. More notably, circRNAs, such as circDdx17 and circARRDC3, can play a role in murine AR development and NEpC damage [4, 9]. Previous research has shown that circRNA ADP-ribosylation factor 3 (circARF3) can reduce inflammatory damage in obesity [10].…”

circARRDC3 contributes to interleukin‑13‑induced inflammatory cytokine and mucus production in nasal epithelial cells via the miR‑375/KLF4 axis

“…Several studies have indicated that circRNA dysregulation is involved in inflammatory-related diseases, including AR. For example, Liu and Cao ( 6 ) reported that circDdx17 downregulated microRNA (miR/miRNA)-17 expression and alleviated AR symptoms and pathological conditions. Zhu et al ( 7 ) indicated that circHIPK3 overexpression accelerated T helper cell (Th)2 differentiation of ovalbumin-induced CD4 + T cells and aggravated the nasal symptoms of AR mice.…”