Protective effect of Danning tablet on acute livery injury with cholestasis induced by α-naphthylisothiocyanate in rats

Ding, Lili; Zhang, Bin-Feng; Dou, Wei; Yang, Li; Chen, Zhan; Wang, Zhengtao

doi:10.1016/j.jep.2011.12.047

Cited by 28 publications

(15 citation statements)

References 32 publications

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“…ALT, AST, and TB are commonly used as biomarkers and are generally increased in chemical toxin-induced liver injury; such toxins include carbon tetrachloride, AP, and ␣-naphthylisothiocyanate (Jayakumar et al, 2006;Ding et al, 2012;Zhao et al, 2011). The results of serum ALT/AST activity, and TB amount analysis demonstrated that FL can prevent AP-induced liver injury.…”

Section: Discussionmentioning

confidence: 99%

Protection of Flos Lonicerae against acetaminophen-induced liver injury and its mechanism

Jiang¹,

Sheng

Chen³

et al. 2014

Environmental Toxicology and Pharmacology

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Section: Discussionmentioning

confidence: 99%

Protection of Flos Lonicerae against acetaminophen-induced liver injury and its mechanism

Jiang¹,

Sheng

Chen³

et al. 2014

Environmental Toxicology and Pharmacology

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“…The bile duct was cannulated with Closed IV Catheter System (inner diameter, 0.36 mm; outer diameter, 0.71 mm; Becton, Dickinson and Co., Suzhou, China). After the operation, Bile specimens were collected into tubes for 1 h, as previously described [18]. Then, the animals were killed by decapitation, the liver and kidney of rats were collected and stored in liquid nitrogen for analysis of enzyme levels and gene or protein expressions.…”

Section: Methodsmentioning

confidence: 99%

“…We previously showed that the protective effect of DNts on ANIT-induced liver injury was probably due to its attenuation of oxidative stress disruption in the liver tissues and neutrophil infiltration [18]. However, the underlying mechanisms about protective effect of DNts on ANIT-induced cholestasis are not explored yet.…”

Section: Introductionmentioning

confidence: 99%

Danning tablets attenuates α-naphthylisothiocyanate-induced cholestasis by modulating the expression of transporters and metabolic enzymes

Ding

Zhang

Chen

et al. 2014

BMC Complement Altern Med

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BackgroundThe Danning tablets (DNts) is commonly prescribed in China as a cholagogic formula. Our previous studies showed that DNts exerted the protective effect on α-naphthylisothiocyanate (ANIT)-induced liver injury with cholestasis in a dose-dependent mannar. However, the detailed molecular mechanisms of DNts against ANIT-induced cholestasis are still not fully explored.MethodsDanning tablet (3 g/kg body weight/day) was administered orally to experimental rats for seven days before they were treated with ANIT (60 mg/kg daily via gastrogavage) which caused cholestasis. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (T-Bil), direct bilirubin (D-Bil) and total bile acid (TBA) were measured to evaluate the protective effect of Danning tablet at 12, 24 and 48h after ANIT treatment. Meanwhile, total bilirubin or total bile acid in the bile, urine and liver were also measured at 48h after ANIT treatment. Furthermore, the hepatic or renal mRNA and protein levels of metabolic enzymes and transports were investigated to elucidate the protective mechanisms of Danning tablet against ANIT-induced cholestasis.ResultsIn this study, we found that DNts significantly attenuated translocation of multidrug resistance-associated protein 2 (Mrp2) from the canalicular membrane into an intracellular and up-regulated the hepatic mRNA and protein expressions of metabolic enzymes including cytochrome P450 2b1(Cyp2b1) and uridine diphosphate-5¢- glucuronosyltransferase (Ugt1a1)) and transporters including bile salt export pump (Bsep) and multidrug resistance protein 2 (Mdr2)) as well as renal organic solute transporter beta (Ostβ), accompanied by further increase in urinary and biliary excretion of bile acid and bilirubin.ConclusionsDNts might promote bile acid and bilirubin elimination by regulating the expressions of hepatic and renal transporters as well as hepatic metabolic enzymes.

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“…22) While SOD catalyzes the conversion of superoxide free radical to less toxic hydrogen peroxide, GPx catalyzes the breakdown of hydrogen peroxide into water and oxygen and can also directly detoxify lipid peroxides generated by ROS. 23) As SOD and GPx are easily inactivated by ROS or lipid peroxides, this may explain a decrease in activities of these two enzymes observed in liver tissue of CCl 4 -intoxicated rats in our study. However, our results demonstrated that SOD and GPx were appreciably elevated by PTA administration, suggesting that it could restore both enzymes and/or activate enzyme activities in CCl 4 -damaged liver tissue.…”

Section: Discussionmentioning

confidence: 52%

Hepatoprotective Effects of the Polysaccharide Isolated from <i>Tarphochlamys affinis</i> (Acanthaceae) against CCl<sub>4</sub>-Induced Hepatic Injury

Lin

Liu

Huang

et al. 2012

Biological & Pharmaceutical Bulletin

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This study was designed to investigate the protective effects of the polysaccharide isolated from Tarphochlamys affinis (PTA) against CCl 4 -induced hepatotoxicity in rats. Liver injury was induced in rats by the administration of CCl 4 twice a week for 2 weeks. During the experiment, the model group received CCl 4 only; the treatment groups received various drugs plus CCl 4 , whereas the normal control group received an equal volume of saline. Compared with the CCl 4 group, PTA significantly decreased the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in the serum and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) in the liver. Moreover, the content of hepatic malondialdehyde (MDA) was reduced. Histological findings also confirmed the antihepatotoxic characterisation. In addition, PTA significantly inhibited the proinflammatory mediators, such as prostaglandin E 2 (PGE 2 ), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) and myeloperoxidase (MPO). Further investigation showed that the inhibitory effect of PTA on the pro-inflammatory cytokines was associated with the down-regulation of nuclear factor-kappa B (NF-κB). In brief, our results show that the protective effect of PTA against CCl 4 -induced hepatic injury may rely on its ability to reduce oxidative stress and suppress inflammatory responses. Key words Tarphochlamys affinis (Acanthaceae); polysaccharide; hepatoprotective effect; carbon tetrachlorideIn recent years, "oxidative stress" and its adverse effects on human health has become a subject of considerable interest of research. It is a well-documented fact that exposure of organisms to the exogenous and endogenous factors generates a wide range of reactive oxygen species (ROS), resulting in the homeostatic imbalance.1,2) Production of free radicals exceeded, such as superoxide, hydroxyl radicals, hydrogen peroxide and nitric oxide (commonly designated as ROS), was found certain to play multiple important roles in tissue damage and the loss of function.3) Development in the biomedicine points to the involvement of the free radicals in many diseases. The free radicals attack the unsaturated fatty acids in the biomembranes, yielding the membrane lipid peroxidation, the decrease in membrane fluidity, the loss of enzymes and the damage to membrane proteins and leading to the cell inactivation.4) The harmful action of the free radicals can, however, be blocked by the antioxidant substances, which scavenge the free radicals and detoxify the organism. Many studies have shown that natural polysaccharide, which were found largely in fruits and vegetables, have been confirmed to play an important part as free radical scavengers in the prevention of oxidative damage in living organism and can be exploited as novel potential antioxidants, and the effects have something to do with their chemical properties and architectural characteristics.5) T...

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Protective effect of Danning tablet on acute livery injury with cholestasis induced by α-naphthylisothiocyanate in rats

Cited by 28 publications

References 32 publications

Protection of Flos Lonicerae against acetaminophen-induced liver injury and its mechanism

Protection of Flos Lonicerae against acetaminophen-induced liver injury and its mechanism

Danning tablets attenuates α-naphthylisothiocyanate-induced cholestasis by modulating the expression of transporters and metabolic enzymes

Hepatoprotective Effects of the Polysaccharide Isolated from <i>Tarphochlamys affinis</i> (Acanthaceae) against CCl<sub>4</sub>-Induced Hepatic Injury

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