Protective Effect of Grape Seed Procyanidins against H<sub>2</sub>O<sub>2</sub>‐Induced Oxidative Stress in PC‐12 Neuroblastoma Cells: Structure–Activity Relationships

Luo, Lanxin; Bai, Ruifang; Zhao, Yuqing; Li, Jing; Wei, Zongmin; Wang, Fang; Sun, Baoshan

doi:10.1111/1750-3841.14349

Cited by 30 publications

(24 citation statements)

References 26 publications

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“…This property was reported for polyphenols present in wine, such as resveratrol, that was shown to inhibit cyclooxygenase 2 (COX-2) and decrease prostaglandin E 2 (PGE 2 ) in the presence of beta-amyloid peptide [69]. Moreover, procyanidins from seed grapes showed antioxidant benefits in PC-12 cells [70]. Our results show that dark beer is the treatment that presents the highest antioxidative effect, at 30 min and 24 h, and is able to produce the highest increase of adenosine A 1 receptor protein levels in both cells models analyzed.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

2019

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The fight against neurodegenerative diseases is promoting the searching of nutrients, preferably of wide consumption, with proven effects on health. Beer is widely consumed and has potential benefits on health. In this work, three different extracts from dark beer (DB), non-alcoholic beer (NAB), and lager beer (LB) were assayed at 30 min and 24 h in rat C6 glioma and human SH-SY5Y neuroblastoma cells in order to study their possible protective effects. Cell viability and adenosine A1, A2A, A2B, and A3 receptor gene expression and protein levels were measured in control cells and in cells challenged with hydrogen peroxide as an oxidant stressor. Among the three extracts analyzed, DB showed a greater protective effect against H2O2-induced oxidative stress and cell death. Moreover, a higher A1 receptor level was also induced by this extract. Interestingly, A1 receptor level was also increased by NAB and LB extracts, but to a lower extent, and the protective effect of these extracts against H2O2 was lower. This possible correlation between protection and A1 receptor level was observed at 24 h in both C6 and SH-SY5Y cells. In summary, different beer extracts modulate, to a different degree, adenosine receptors expression and protect both glioma and neuroblastoma cells from oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

2019

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“…The phenols in Vitis vinifera L. seeds provide health benefits and proanthocyanidins, also known as condensed tannins, play a potential nutraceutical role. The antioxidant and free radical scavenging abilities of phenols have been demonstrated both in vivo and in vitro, protecting more than vitamins E or C and β-carotene [ 42 , 43 , 44 ]. Other activities—such as anti-inflammatory [ 45 ], antimicrobial [ 46 ], antiulcer [ 47 ], and anticancer—have also been reported [ 48 , 49 , 50 ].…”

Section: Grapes Seeds and Seed Extracts: Natural Sources Of Nutrmentioning

confidence: 99%

Grape (Vitis vinifera L.) Seed Oil: A Functional Food from the Winemaking Industry

Martín

Grao‐Cruces

Millán-Linares

et al. 2020

Foods

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Wine production is an ancient human activity that generates several by-products, which include some constituents known for their potential in health care and for their role in the food or cosmetic industries. Any variety of grape (Vitis vinifera L.) contains nutrients and bioactive compounds available from their juice or solid parts. Grape seed extract has demonstrated many activities in disease prevention, such as antioxidant effects, which make it a potential source of nutraceuticals. Grape seed is a remarkable winery industry by-product due to the bioactivity of its constituents. Methods for recovery of oil from grape seeds have evolved to improve both the quantity and quality of the yield. Both the lipophilic and hydrophilic chemicals present in the oil of V. vinifera L. make this wine by-product a source of natural nutraceuticals. Food and non-food industries are becoming novel targets of oil obtained from grape seeds given its various properties. This review focuses on the advantages of grape seed oil intake in our diet regarding its chemical composition in industries not related to wine production and the economic and environmental impact of oil production.

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“…Procyanidins, the water-soluble and fat-soluble compounds, are the most abundant polyphenols found in foods such as fruits, red wine, green tea (C adiz-Gurrea et al 2014) and in bark and the seeds of many plants (Luo et al 2018). Procyanidins arise from flavan-3-ol units, including catechin and epicatechin, and exist in a range of forms (dimers, trimers, tetramers, pentamers, and polymers).…”

Section: Discussionmentioning

confidence: 99%

“…Among all types of procyanidins, the dimers are the most abundant in nature and have stronger antioxidant activities. Procyanidins also have anti-inflammatory (Youn et al 2017), antioxidant (Luo et al 2018), and hypoglycaemic properties (Gonzalez-Abuin et al 2015). Therefore, they are used in the prevention and treatment of atherosclerosis, coronary heart diseases (Goszcz et al 2017), DM (Gonzalez-Abuin et al 2015;Ogura et al 2016), and cancers (Choy et al 2016).…”

Section: Discussionmentioning

confidence: 99%

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Screening active components from Rubus amabilis for pancreatic β-cells protection

Sun

Zhu

Tong

et al. 2020

Pharmaceutical Biology

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Context: Rubus species (Rosaceae) have been used in folk medicine to treat diabetes due to their hypoglycaemic activity. Objective: To screen the active components that act as hypoglycaemic agents in Rubus amabilis Focke and the underlying mechanisms. Materials and methods: Aqueous stem extract of R. amabilis was incubated with MIN6 b-cells, PBS was used as the blank control. Then the cells were washed, cell membrane-bound components were dissociated and identified by UPLC/MS. Total procyanidins (PCs) in R. amabilis was enriched and the cytotoxicity and anti-proliferation on b-cell were evaluated by MTT assay. PCs at 25, 50, and 75 lg/mL was applied for 24 h to determine its effects on palmitate (PA)-induced apoptosis and GSIS. Western blotting was employed to detect the protein expression of PI3K/Akt/FoxO1 signalling. The antioxidant indices were also measured. Results: b-Cell membrane-bound components were identified as three procyanidin B dimers and a C trimer. PCs showed no significant cytotoxicity up to a concentrations of 100 lg/mL. PCs treatment reversed the elevated apoptosis rate and impaired GSIS induced by PA. PCs markedly decreased the intracellular ROS and MDA production and increased the SOD activity. Moreover, PCs promoted the phosphorylation of Akt and FoxO1, and regulated Pdx-1 and Bax expression in MIN6 cells. Discussion and conclusion: The active components that act as hypoglycaemic agents in R. amabilis are procyanidins, which protected MIN6 cells against PA-induced apoptosis by activating PI3K/Akt/FoxO1 signalling. These results indicate that b-cell extraction, combined with UPLC/MS, is a valid method for screening antidiabetic components from herbal medicines.

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Protective Effect of Grape Seed Procyanidins against H₂O₂‐Induced Oxidative Stress in PC‐12 Neuroblastoma Cells: Structure–Activity Relationships

Cited by 30 publications

References 26 publications

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Grape (Vitis vinifera L.) Seed Oil: A Functional Food from the Winemaking Industry

Screening active components from Rubus amabilis for pancreatic β-cells protection

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Protective Effect of Grape Seed Procyanidins against H2O2‐Induced Oxidative Stress in PC‐12 Neuroblastoma Cells: Structure–Activity Relationships

Cited by 30 publications

References 26 publications

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models

Grape (Vitis vinifera L.) Seed Oil: A Functional Food from the Winemaking Industry

Screening active components from Rubus amabilis for pancreatic β-cells protection

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Product

Resources

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Protective Effect of Grape Seed Procyanidins against H₂O₂‐Induced Oxidative Stress in PC‐12 Neuroblastoma Cells: Structure–Activity Relationships