Protective effect of leaf extract of Abutilon indicum on DNA damage and peripheral blood lymphocytes in combating the oxidative stress

Abstract

The current research explores in vitro antioxidant characteristics, radiation-induced DNA damage protection and quenching effects of the oxidative stress by the ethanolic leaf extract of Abutilon indicum (EEAI) on human peripheral blood lymphocytes (PBLs). PBLs were incubated with various concentrations of EEAI accompanied by pre- and post-treatment with hydrogen peroxide. Cell viability was investigated by MTT assay. In addition, quenching of free radicals were measured in vitro using DPPH, superoxide anion, hydrogen peroxide, reducing power and nitric oxide radical scavenging assays. These activities were compared with ascorbic acid as standard antioxidants. Furthermore, inhibition of UV radiation-induced strand break formation in plasmid pBR322 DNA and anti-Fenton reactions in calf thymus DNA was evaluated. Cytotoxic effects of hydrogen peroxide on PBLs were significantly reduced with EEAI pre-treatment compared to post-treatment in a dose-dependent manner comparable with similar cytoprotective effects of ascorbic acid ( p > 0.05). EEAI has shown strong antioxidant effects in the scavenging of DPPH, superoxide anion, hydrogen peroxide, and nitric oxide. EEAI also has a strong protective effect of UV-induced plasmid pBR322 DNA cleavage and Fenton-induced DNA damage. Overall, the results revealed that Abutilon indicum has a cytoprotective, potent antioxidant and DNA protective effect that provide pharmacological credence to justify its overall biological activity. Furthermore, future studies to identifying bioactive molecules and its molecular mechanisms responsible for promising therapeutic applications in the rescue of disease-induced cellular oxidative damage.

Exploring the excellence of commercial Brahmi products from Thai online markets: Unraveling phytochemical contents, antioxidant properties and DNA damage protection

“…High amounts of phenolic compounds in the P6 sample with strong antioxidant properties were positively associated with DNA protection against oxidative stress [ 47 ]. These results agreed with previous findings that H 2 O 2 -induced plasmid DNA cleavage decreased in the presence of bacopa extracts [ 13 , 48 ], Mentha arvensis [ 49 ], Abutilon indicum [ 50 ], and Terminalia catappa [ 51 ]. One possible explanation is that bacopa extracts act as protective agents against H 2 O 2 -induced DNA damage by reducing the scavenging of highly reactive hydroxyl radicals [ 47 ].…”

Effects of Spirulina platensis on Iron Oxide Nanoparticles Induced-oxidative Stress and Liver Damage in Grey Mullet (Mugil cephalus)

“…The findings were consistent with those of Hussein et al (2019), who indicated that oxidative stress and apoptosis might play a role in neurotoxicity, which disrupted GSH In this research, a decrease in GSH levels may be attributable to its conjugation with certain metabolites (Wu & Dhanasekaran, 2020). Hassan et al (2012), who investigated spirulina's efficacy in combating oxidative stress in rats, found a comparable rise in GSH levels.…”

Phytochemical Characterization and Assessment of Crude Extracts From Lantana camara L. for Antioxidant and Antimicrobial Activity

“…The ability of extracts to protect DNA (Plasmid DNA pGEM-T) from the damaging effect of hydroxyl radicals generated by Fenton's reagent was evaluated by DNA nicking test with some modification (Wu and Dhanasekaran, 2020). The reaction mixture was prepared by adding 4.0 µl of plasmid DNA (100 ng/µl), 10 µl of Fenton's reagent (30 mM H 2 O 2 , 100 µl ascorbic acid and 200 µM FeCl 3 ), and 5.0 µl of extracts (5 mg/ml) and final volume was made to 20 µl with double distilled water.…”