Protective effect of oil extracted from Neophocaena asiaeorientalis against hydgrogen peroxide-induced oxidative stress in HepG2 cells

Kim, Hye Ryeong; Kim, Ji Eun; Yun, Won Young; Lee, Mi Rim; Choi, Jun Young; Park, Jin Ju; Song, Bo; Song, Hyun Keun; Hwang, Dae Youn

doi:10.1007/s12562-019-01329-9

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…Therefore, a moderate concentration of H 2 O 2 close to IC 50 is generally considered to establish the oxidative damage model. [23] The cell viability of HUVECs was 52.23 % when the H 2 O 2 concentration was 30 μg/mL, which was close to IC 50 . Therefore, 30 μg/mL was determined to be the appropriate modeling concentration for subsequent experiments.…”

Section: Establishment Of H 2 O 2 -Induced Huvecs Oxidative Damage Modelsupporting

confidence: 52%

Protective Effect of Cannabidiol on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)

Yang

Zhao

et al. 2023

Chemistry & Biodiversity

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Natural antioxidants play an important role in promoting good health because of their prevention for oxidative damage. The work aimed to explore the antioxidant mechanism and activity of cannabidiol (CBD) at the cellular level. The human umbilical vein endothelial cell (HUVEC) with oxidative damage was employed as the model to study the protective capability of CBD. The results showed that CBD pre-treatment before the cells were exposed to hydrogen peroxide (H 2 O 2 ) resulted in an obvious increase of cell viability (about 100 %) and antioxidant related enzymes activity, and a decline of malondialdehyde (MDA) level. Besides, CBD could alleviate the increase of intracellular reactive oxygen species (ROS) content, the contraction of nucleus, and condensation of chromatin. The changes showed a dose-dependent effect. Additionally, the free radicals scavenging capacity of CBD was comparable to that of typical natural antioxidant, anthocyanidins. In summary, CBD could be employed as a potent antioxidant source for avoiding the oxidative damage. These results could provide the foundation for the development of CBD antioxidant products.

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Section: Establishment Of H 2 O 2 -Induced Huvecs Oxidative Damage Modelsupporting

confidence: 52%

Protective Effect of Cannabidiol on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)

Yang

Zhao

et al. 2023

Chemistry & Biodiversity

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“…The disorder of apoptotic process has bad effects on the body and causes many diseases [ 32 ]. H 2 O 2 , as an important signaling molecule, regulates the process of cell proliferation, growth, and apoptosis [ 5 ]. The present study measured the apoptosis of H 2 O 2 -induced HepG2 cells and evaluated the cytoprotective effects of compounds 2 , 5 , and 6 .…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress is closely related to a variety of diseases including diabetes, hyperlipemia, obesity, cancer, and cardiovascular and neurodegenerative diseases [ 4 ]. Recently, many studies have evidenced that a diet enriched with antioxidants possesses a series of beneficial effects owing to their scavenging ability on excessive ROS [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Activity Guided Isolation of Phenolic Compositions from Anneslea fragrans Wall. and Their Cytoprotective Effect against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells

Cui

Khan

et al. 2021

Molecules

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Anneslea fragrans Wall., commonly known as “Pangpo Tea”, is traditionally used as a folk medicine and healthy tea for the treatment of liver and intestine diseases. The aim of this study was to purify the antioxidative and cytoprotective polyphenols from A. fragrans leaves. After fractionation with polar and nonpolar organic solvents, the fractions of aqueous ethanol extract were evaluated for their total phenolic (TPC) and flavonoid contents (TFC) and antioxidant activities (DPPH, ABTS, and FRAP assays). The n-butanol fraction (BF) showed the highest TPC and TFC with the strongest antioxidant activity. The bio-guided chromatography of BF led to the purification of six flavonoids (1–6) and one benzoquinolethanoid (7). The structures of these compounds were determined by NMR and MS techniques. Compound 6 had the strongest antioxidant capacity, which was followed by 5 and 2. The protective effect of the isolated compounds on hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells revealed that the compounds 5 and 6 exhibited better protective effects by inhibiting ROS productions, having no significant difference with vitamin C (p > 0.05), whereas 6 showed the best anti-apoptosis activity. The results suggest that A. fragrans could serve as a valuable antioxidant phytochemical source for developing functional food and health nutraceutical products.

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Protective effect of oil extracted from Neophocaena asiaeorientalis against hydgrogen peroxide-induced oxidative stress in HepG2 cells

Cited by 2 publications

References 33 publications

Protective Effect of Cannabidiol on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)

Protective Effect of Cannabidiol on Hydrogen Peroxide‐Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)

Activity Guided Isolation of Phenolic Compositions from Anneslea fragrans Wall. and Their Cytoprotective Effect against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells

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