Protective Effect of Resveratrol against Oxidation Stress Induced by 2-Nitropropane in Rat Liver

Abstract

We investigated the effect of resveratrol on oxidation damage and variation of antioxidant defences induced by 2-nitropropane (2-NP) in rat liver. One group of five rats was given resveratrol (50 mg/kg/d body weight) in the diet until the end of the experiment. After 14 days, 2-NP (100 mg/kg) was injected i.p. into two groups of animals (2-NP + Res and 2-NP groups) while control animals were treated with vehicle alone. Animals were killed by decapitation 15 h after 2-NP injection. The levels of 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodGuo) were significantly increased by 2-NP injection, but resveratrol restored 8-oxodGuo to levels similar to those measured in controls. Superoxide dismutase (SOD) and xanthine oxidase (XO) activities in the liver were significantly increased by 2-NP, but were similar to those found in the group treated with resveratrol and 2-NP (2-NP + Res). We also observed that 2-NP injection significantly reduced GSH/GSSG ratio in the liver and this change was partially reversed by resveratrol treatment. Moreover, an increased (p = 0.06) expression of the oxoguanine glycosylase (OGG1) gene was found in 2-NP rats, whereas pre-treatment with resveratrol restored OGG1 expression to control levels. An up-regulation of caspase-3 was also observed in 2-NP group, but resveratrol significantly reduced the activation of caspase-3. An inverse correlation was found between GSH/GSSG and 8-oxodGuo in the 2-NP group. On the contrary, 8-oxodGuo levels, GSH/GSSG ratio, XO and SOD activities in the colon mucosa of 2-NP rats were similar to those of controls confirming that the colon is not a target of oxidation damage 2-NP induced. In conclusion, our results indicate that oxidative DNA damage and apoptosis are the main mechanisms of cell death in a model of chemically induced severe acute hepatic injury and in this early stage of damage pharmacological doses of resveratrol can ameliorate hepatic oxidation damage by its antioxidant and scavenging properties through a reduction of XO activity, a partial restoration of GSH/GSSG ratio in addition to its capacity to inhibit apoptosis

Resveratrol and liver: A systematic review

“…In a model of chemically induced acute hepatic injury, resveratrol at dose of 50 mg/kg/day administered for 14 days long could ameliorate hepatic injury by its antioxidant and scavenging properties, through a reduction of xanthine oxidase activity, a partial restoration of GSH/GSSG ratio in addition to its capacity to inhibit apoptosis. [ 44 ]…”

Moringa oleifera fruit extract as a potential antioxidant against liver injury by 2-Nitropropane induction in obese male mice model: pre-clinical study

“…6,32,33 Induction of 2-Nitropropane can cause liver cell damage and acute liver injury, which was characterized by increased levels of SGOT and SGPT. 18,27 When given M. oleifera fruits extract, there was a decrease in liver SGOT and SGPT levels in groups P1 and P2 compared to groups O1 and O2. These findings indicate that M. oleifera extract can be a potential antioxidant candidate in liver damage due to 2-Nitropropane induction.…”

“…The dose of 2-Nitropropane (130263-5ml Aldrich ® ) of 0.02 mg/kgBW was given intraperitoneally to induce oxidative stress and liver injury in male obese mice models. 27…”

“…26 2-Nitropropane (2NP) can trigger the mechanism of hepatocarcinogenesis. 17,24,27 Research by Metwally et al (2017) in obese female rats given M. oleifera at a dose of 600 mg/kgBW orally for 12 weeks is a good candidate for therapy for symptoms of metabolic syndrome. 8 There have been many studies reporting on M. oleifera fruit.…”

Chitosan nanoparticles as a promising candidate for liver injury induced by 2-nitropropane: Implications of P53, iNOS, VEGF, PCNA, and CD68 pathways

“…The current treatment modality for 2-NP-mediated liver injury is extensively investigated. The protective effect of resveratrol, 32 binaphthyl diselenide, 33 or moringa oleifera 34 as a treatment for 2-NP induced hepatotoxicity was documented. CNPs are well-recognized delivery systems broadly used as polymeric carriers in the nanomedicine field.…”