Protective Effect of the Leaf Extracts of Combretum racemosum P. Beauv (Combretaceae) on Cyclophospahamide Induced Pancytopaenia and Liver Injury in Male Rats

Okwuosa, Chukwugozie N; Achukwu, Peter Uwadiegwu; Azubike, N.C.; ., A.I.E. Abah

doi:10.3923/rjpharm.2012.30.34

Cited by 6 publications

(10 citation statements)

References 3 publications

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“…Other research works corroborate with this one (Lakshmi, Rudrama, & Madhavi, 2009;Saber, Hoda, & Hawazen, 2011;Okwuosa, Achukwu, Azubike, & Abah, 2012). It was also noticed that depending on the extract (hydroethanolic extracts of S. scabrum and C. verticillata) and on the dose (200 or 400 mg/kgBW) associated to cyclophosphamide, the level could be increased or decreased depending on the biochemical parameter evaluated.…”

Section: Discussionsupporting

confidence: 71%

Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Mbong¹,

Djiokeng²,

Ntentie³

et al. 2014

JFR

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The aim of this study was to evaluate the protective effects of hydroethanolic extracts of S. scabrum and C. verticillata against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of C. verticillata/S. scabrum at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of S. scabrum and C. verticillata can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.

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Section: Discussionsupporting

confidence: 71%

Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Mbong¹,

Djiokeng²,

Ntentie³

et al. 2014

JFR

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“…Rotimi et al, [22] which found the cobalt ion (CO +2 ) a better activator of rat kidney ALP, the activation may be through formation of an activated (CO +2 ) ALP complex where (CO +2 ) occupies both catalytic and structural sites of alkaline phosphatase. The new cobalt (II) complex was similar effect to cyclophosphamide (cp) that could be attributed to the cp exhibit greates cytotoxicity against cell actively replicating .The DNA as umpiring of DNA strands at this stage makes the nucleotide residues more susceptable to alkylation, hepatic activation of cp leading to the formation of toxic metabolites caused damage to the liver tissues as shown by increased ALP [8]. Conclusion: The study showed the cobalt (II) complex have a cytotoxic effect by reducing the GPT activity and activation of ALP activity at different concentrations these effect were similar the effect of cp at the same doses .…”

Section: -Lungmentioning

confidence: 84%

“…Several alkyne cobalt carbonyl complexes inhabited the growth of human melanoma and lung carcinoma cell lines [5] .Roth et al [6] Cyclophosphamide a nitrogen mustard, is an alkylating agent from the oxazophosphorine group [7]. It is widely used chemotherapeutic agent, it undergos extensive metabolism via the cytochrome P450 enzymatic system with phosphamide mustard and acrolein as the main active and inactive metabolites [8].Previous studies had shown that this compound is relatively inactive in vitro and is converted to the active form in vivo, trials in various tumorbearing animals confirmed this in vivo activity and demonstrated fairly potent antitumor effect [9]. Enzymes are necessary for normal cellular metabolism including that of the liver, and the degenerative changes due to the combined metal toxicity exhibited in the liver alter the level of a number of its enzymes [10].…”

Section: Introductionmentioning

confidence: 99%

A study of the effect of new cobalt (II) complex and cyclophosphamide drug on (GPT, ALP) activity by using in vivo system

Murtadha¹

2015

Baghdad Sci.J

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The present work involved a study the effect of cobalt(II) complex with formula [CoL(H2O)NO3] .4ETOH where L=Nitro [5-(P-nitro phenyl) -4-phenyl-1,2,4 traizole-3-dithiocarbamato hydrazide] aqua. (4) Ethanol and anti-cancer drug - cyclophosphamide on specific activity of two liver enzymes (GPT,ALP) by utilizing an in vivo system in female mice. On the enzymatic level an inhibition in the activity of GPT was noticed in different body organs such as liver, kidney and lung. The inhibition was noticed in both test and cyclophosphamide drug (cp). Mice were treated with three doses of cyclophosphamide (90,180, 250) ?g/ mouse for three days. The same doses were used for the cobalt (II) complex. The liver shows the highest rate of(GPT) inhibition compared to other organs. The ratio was about 90% at three doses of cobalt (II) complex, this ratio was similar to ratio inhibition of cyclophosphamide at the same doses. On the contrary the enzyme ALP showed high activation in different organs such as liver, kidney and lung in both groups, test and cyclophosphamide drug (cp) at the three doses (90, 180, 250) ?g /mouse. The result showed the highest ratio of activation in the kidney comparable with other organs. The maximum activation of cobalt(II) complex was about 1198% at a concentration 180µg/mouse.There are significant differences(P

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“…Species of Combretum genus has been of interest in the last two decades because of the isolation of some compounds with highly significant activities in anticancer and antiinfectives models isolated from this genus (Okwuosa et al, 2012;Pettit et al, 1997). This has made the combretum genus a very important one in search of bioactive compounds for the treatment and management of disease conditions that are related to Reactive Oxygen Species (ROS).…”

Section: Introductionmentioning

confidence: 99%

“…It is used locally for the treatment of ulcers, infections, among other diseases. There are also recent reports of the anticancer potential of this species (Okwuosa et al, 2012). Current study investigated the antioxidant potential of Combretum racemosum extract and its fractions which led to the isolation of a compound from the most active fraction.…”

Section: Introductionmentioning

confidence: 99%

Bioguided isolation of an antioxidant compound from <i>Combretum racemosum</i> P.Beav leaf

Babatunde¹,

Moyinoluwa²,

Oluwatosin³

et al. 2015

Int. J. Bio. Chem. Sci

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Free radicals are known to cause damage to the body cells leading to many diseases. These cells can be protected from such deleterious effects by antioxidants; therefore, plant derived antioxidants are of global interest. Combretum racemosum have been used traditionally in the treatment of ulcers, cancers and infections. Current studies investigated the antioxidant activities of this plant extract and its fractions. The antioxidant activity of the crude extract was determined by Ferric Reducing Power Assay, DPPH free radical scavenging assay, Total Phenolic and Total Flavonoid Content methods. Bioguided column chromatographic separation was carried using DPPH free radical scavenging assay to assess activity of fractions. A bioactive compound was isolated and characterized by application of Spectroscopic techniques (ESI-MS, HRMS, 1D and 2D NMR). Bioactivity of the crude extract showed that Combretum racemosum has good antioxidant activity which is comparable to the reference antioxidant (catechin). Bioactivity guided fractionation using DPPH method led to the isolation of 2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5,7-triol as an active principle from the extract. This study suggests that there is a scientific basis for the application of C. racemosum extract in the management of diseases that are associated with cell damage caused by free radicals.

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Protective Effect of the Leaf Extracts of Combretum racemosum P. Beauv (Combretaceae) on Cyclophospahamide Induced Pancytopaenia and Liver Injury in Male Rats

Cited by 6 publications

References 3 publications

Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

A study of the effect of new cobalt (II) complex and cyclophosphamide drug on (GPT, ALP) activity by using in vivo system

Bioguided isolation of an antioxidant compound from <i>Combretum racemosum</i> P.Beav leaf

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