Protective effect of β-glucan on acute lung injury induced bylipopolysaccharide in rats

Abstract

Background/aim: Lipopolysaccharide (LPS)-induced endotoxemia can cause serious organ damage such as acute lung injury and death by triggering the secretion of proinflammatory cytokines and acute-phase reactants. The goal of this study was to evaluate the effects of β-glucan on inflammatory mediator levels and histopathological changes in LPS-induced endotoxemia. Materials and methods:Forty-seven male Wistar albino rats were randomly allocated into four groups as follows: control group, LPS group (10 mg/kg LPS), LPS + β-glucan group (100 mg/kg β-glucan before LPS administration), and β-glucan group. Twelve hours after LPS administration, lung and serum samples were collected. Concentrations of IL-6, IL-8, C-reactive protein (CRP), and procalcitonin were measured in the serum at hours 0 (basal) and 12. The severity of lung damage was assessed by an appropriate histopathological scoring system. Results: Serum levels of CRP in the LPS group at 12 h were significantly higher than in the other groups, whereas serum IL-6 levels in the LPS and LPS + β-glucan groups at 12 h were significantly decreased. The mean histopathological damage score of the LPS group was slightly higher than that of the LPS + β-glucan group. Moreover, mortality rate was significantly decreased in the LPS + β-glucan group versus the LPS group.Conclusion: β-Glucan reduces endotoxemia-induced mortality and might be protective against endotoxemia-induced lung damage.

Protective Effects of Curcumin on Cadmium-Induced Renal Injury in Young and Aged Rats

“…C-reactive protein is a marker, which appears during the late phase of infection, whereas IL-6 and PCT are affected in the early phase (36)(37)(38). In the present study, increased PCT levels of Cd-administered young (p=0.004) and aged (p=0.004) rats were reduced by Cr administration.…”

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

“…CRP levels in serum increase during infection and inflammation (33). C-reactive protein is a marker, which appears during the late phase of infection (34,35). In the present study rats chronically exposed to a wide range of arsenic concentrations through drinking water, arsenic exposure was associated with increased serum CRP levels in arsenictreated rats compared with the control (p < 0.05) ( Figure 3).…”

Effect of fluid resuscitation on acute lung injury in a rat model of sepsis

“…Some studies indicate that CRP may be an indication of progressive organ damage (45)(46)(47). ARDS is an infl ammatory process and many studies have centered on determining the function of infl ammatory plasma mediators in this disease and how they correlate with phenotypes and clinical outcomes (18,48,49). While literature studies associating CRP levels with prognoses in other diseases have been performed, there is little knowledge of the CRP function in ARDS and ALI patients.…”

“…All rats were anesthetized by ketamine (40 mg/kg, Alfamine, Alfasan International B.V., The Netherlands) and xylazine (4 mg/kg, Alfazyne, Alfasan International B.V., The The basic histopathological lung injury score was calculated as used in previous publications (18). In a nutshell, histopathological lung damage was assessed by scoring alveolar congestion (AC), hemorrhage (H), infi ltration or aggregation of leukocytes in air spaces/vessel walls (AL), perivascular/interstitial edema (PE), and thickness of the alveolar wall/hyaline membrane formation (TA) (18). For all cases, the signifi cance was graded on a scale of 1 (0-25 %), 2 (25-50 %), 3 (50-75 %), and 4 (75-100 %).…”