Protective effects against H2O2-induced oxidative damage in lung fibroblast cell by peptide isolated from plasma albumin hydrolysate

Wang, Jinzhi; He, Yong; Zhang, Hao; Zhang, Chunhui; Luo, Jun; Ren, Fazheng

doi:10.1007/s00217-015-2418-x

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…Cell viability of the damaged Caco‐2 cells treated with F5 and those treated with the control group was tested by 3‐(4, 5‐dimethyl thiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay (Wang et al, 2012 ). The experiment involved first incubating F5 and complete medium for 4 h, and then using 10 mM H 2 O 2 to damage Caco‐2 cells for 6 h on 96‐well round‐bottom microplates (Wang et al, 2015 ). The control group (without F5 or H 2 O 2 ) was incubated with complete medium for 10 h. The microplates were put in a 5% CO 2 cell incubator at 37°C for 4 h after adding 20 μl 5 mg/ml MTT solution.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates

Huang

Jianwei

Yang

et al. 2022

Food Science & Nutrition

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Peptides from oysters have several bioactive functions. In this study, we identified antioxidant peptides from oysters (Crassostrea rivularis) and investigated their structure–function relationship. We used an 8 kDa molecular‐weight (MW) cut‐off membrane and semiprep reversed‐phase liquid chromatography to collect five peptides (F1–F5) and identified the highest‐abundance ion‐peak sequences AWVDY (F1), MSFRFY(F2), EPLRY(F3), RKPPWPP(F4), and YAKRCFR(F5) having MWs of 652, 850, 676, 877, and 943 Da, respectively, using ultra‐performance liquid chromatography‐quadrupole/time‐of‐flight tandem mass spectrometry. These peptides exhibited high antioxidant activities, similar to butylated hydroxytoluene, reduced glutathione, and ascorbic acid. F5 demonstrated the highest scavenging activity for DPPH radicals (IC50 = 21.75 μg/ml), hydroxyl radicals (IC50 = 18.75 μg/ml), and superoxide radicals (IC50 = 11.00 μg/ml), while F3 demonstrated the highest reducing power. Furthermore, F5 significantly protected Caco‐2 cells from H2O2‐induced oxidative damage. These results suggest that the antioxidant peptide F5 is a promising food additive that protects against oxidative damage.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates

Huang

Jianwei

Yang

et al. 2022

Food Science & Nutrition

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“…The hydrolyzed proteins require purification in order to identify their peptide sequences (Figure ). The hydrolysates are initially fractionated through an ultrafiltration membrane system with a molecular weight cut‐off of 1 to 10 kDa (Millipore, Billerica, MA, USA) (Fang et al., ; Liu, Liu, Liu, Xu, & Zhang; Wang et al., ). The fractioned hydrolysates are screened for neuroprotective effects using diverse assay methods, and then novel peptides with neuroprotective effects are purified by various methods to isolate neuroprotective peptides.…”

Section: Production Of Neuroprotective Peptidesmentioning

confidence: 99%

Mechanisms of Neuroprotective Effects of Peptides Derived from Natural Materials and Their Production and Assessment

Lee

2019

Comp Rev Food Sci Food Safe

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In this review, we provide an overview of the main mechanisms by which peptides derived from natural materials exhibit neuroprotective effects. We also review methods for the production of these peptides and various methods for determining their neuroprotective effects in vitro and in vivo. Neuroprotective peptides are closely associated with protection against apoptosis, with proteins related to the cell death/survival signaling pathway, with acetylcholine activity and with inhibition of oxidative stress and inflammation. Neuroprotective peptides derived from natural materials typically have a molecular weight below 1 kDa; they are most often derived using the extraction enzymes papain and alcalase. The neuroprotective peptides identified in the literature are mainly composed of hydrophobic amino acids with low molecular weight, with Asp and Glu at the N‐terminal position. These observations may guide the development of novel peptides with potential neuroprotective effects.

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Protective effects against H2O2-induced oxidative damage in lung fibroblast cell by peptide isolated from plasma albumin hydrolysate

Cited by 2 publications

References 47 publications

Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates

Isolation and characterization of antioxidant peptides from oyster (Crassostrea rivularis) protein enzymatic hydrolysates

Mechanisms of Neuroprotective Effects of Peptides Derived from Natural Materials and Their Production and Assessment

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