Protective effects of a polysaccharide from Boletus aereus on S180 tumor-bearing mice and its structural characteristics

Zheng, Jinhua; Zhang, Tingting; Fan, Jie; Zhuang, Yongliang; Sun, Liping

doi:10.1016/j.ijbiomac.2021.07.191

Cited by 17 publications

(8 citation statements)

References 49 publications

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“…Among these, the thymus and spleen are essential immune organs for lymphocyte differentiation, maturation, and immune response ( 28 , 36 ). Previous studies have shown that CTX can reduce thymus and spleen indices in mice, and natural polysaccharides can increase these indices, suggesting that polysaccharides can protect the body’s immune organs ( 37 – 39 ). Our results are consistent with previous studies, suggesting that MSCP increases host immune function by stimulating immune organ development.…”

Section: Discussionmentioning

confidence: 97%

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Chen

Sun

et al. 2021

Front. Immunol.

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Cyclophosphamide (CTX), a common anticancer drug, can cause a variety of side effects such as immunosuppression and intestinal mucosal injury. Polysaccharides are the major bioactive components of the roots of Millettia Speciosa Champ and have gained attention for their immunomodulatory activity. This study was designed to evaluate the immunomodulatory effect of Millettia Speciosa Champ polysaccharide (MSCP) on CTX-induced mice and the possible mechanism. The results showed that MSCP attenuated the CTX-induced decrease in body weight and immune organ indices in mice and promoted the secretion of immune-related cytokines (IL-2, IL-4, IL-10, TNF-α, and IgG). Meanwhile, MSCP restored intestinal morphology, increased the ratio of villus height/crypt depth (V/C), and improved the number of goblet cells and mucins expression. At the mRNA level, MSCP activated the TLRs/MyD88/NF-κB p65 pathway and enhanced the expression of genes related to intestinal mucosal integrity (Occludin1, Claudin1, and MUC-2). In addition, MSCP as a prebiotic improved microbial community diversity, regulated the relative abundance of dominant microbiota from the phylum level to the genus level, restored CTX-induced gut microbial dysbiosis, and promoted short-chain fatty acid production in mice. Based on the present findings, MSCP may modulate the immune response depending on enhancing intestinal health, suggesting that MSCP holds promise as a promising immunostimulant in functional foods and drugs.

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Section: Discussionmentioning

confidence: 97%

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Chen

Sun

et al. 2021

Front. Immunol.

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“…After 15 days of continuous intragastric administration, 2.5 × 10 6 H22 hepatocarcinoma cells (each mouse) were subcutaneously inoculated in the right forelimb of mice (except the blank group). Cyclophosphamide (CTX), as a chemotherapy drug for the treatment of a variety of cancer diseases, could effectively inhibit tumor growth and was usually used orally or intravenously [ 16 ]. Therefore, CTX was used as a positive control in this study, and mice were injected intraperitoneally with 30 mg/kg CTX daily after tumor induction [ 17 ], and mice in the dose group were given intragastric administration for another 15 days [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Preparation of Water-Soluble Acetylaminoglucan with Low Molecular Weight and Its Anti-Tumor Activity on H22 Tumor-Bearing Mice

Zhang

Dai

2022

Molecules

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In this study, a novel low molecular weight of acetylaminoglucan (AGA) was obtained and its antitumor activity on H22 tumor-bearing mice was investigated. The results of UV, HPLC and FT-IR showed that AGA present high purity with low molecular weight of 2.76 × 103 Da. Animal experiments showed that AGA could inhibit the proliferation of tumor cells in H22 tumor-bearing mice by protecting the immune organs, enhancing the phagocytosis ability of macrophages, killing activity of NK cells and proliferation capacity of lymphocytes, improving the levels of cytokines in vivo and regulating the distribution of lymphocyte subsets, and the tumor inhibition rate reached to 52.74% (50 mg/kg). Cell cycle determination further indicated that AGA could induce apoptosis of tumor cells and arrests it in S phase. These results will provide a data basis for the potential application of AGA in pharmaceutical industry.

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“…Besides, this combination usage of the methods was found to be convenient for the study in the lab with high efficiency, and they had also been employed in the research of separation and purification of mushroom polysaccharides. [12,30,31]…”

Section: Separation and Purification Of Repmentioning

confidence: 99%

Extraction, Bioactive Composition, and Antioxidant Activity of Polysaccharides from the Mushroom Rugiboletus extremiorientalis

Guo

Guo²,

et al. 2022

Starch Stärke

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The wild, edible mushroom Rugiboletus extremiorientalis has an excellent taste and also medicinal effects, and is very popular in China. In this study, hot water extraction is used to isolate polysaccharides from R. extremiorientalis fruiting bodies, and response surface methodology is used to optimize the extraction conditions in terms of temperature, liquid–solid ratio, and time. The crude extract (REP) is purified in turn by DEAE‐52 chromatography and Sephadex G‐100 chromatography, resulting in three fractions (REP‐1, REP‐2, and REP‐3) with recovery rates of 16.7%, 57.65%, and 6.63%, respectively. In vitro antioxidant activity is highest for REP‐2, and the subsequent research is therefore focused on that fraction. High‐performance liquid chromatography reveals that REP‐2 is comprised of fucose, arabinose, galactose, glucose, xylose, and mannose. Fourier‐transform infrared spectroscopy of REP‐2 indicates the presence of functional groups typical for polysaccharides. High‐performance gel filtration chromatography indicates the REP‐2 has a molecular weight of 342.4 kDa. The results of this study provide a foundation for future research concerning the antioxidant activity of polysaccharides in R. extremiorientalis.

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Protective effects of a polysaccharide from Boletus aereus on S180 tumor-bearing mice and its structural characteristics

Cited by 17 publications

References 49 publications

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Preparation of Water-Soluble Acetylaminoglucan with Low Molecular Weight and Its Anti-Tumor Activity on H22 Tumor-Bearing Mice

Extraction, Bioactive Composition, and Antioxidant Activity of Polysaccharides from the Mushroom Rugiboletus extremiorientalis

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