Protective effects of ascorbic acid and vitamin E on antioxidant enzyme activity of freeze-thawed semen of Qinchuan bulls

Zhao, X.-L.; Li, Y.K.; Cao, Sean; Hu, Jianhong; Wang, W.H.; Hao, Ruyi; Gui, Lin; Liu, Zan

doi:10.4238/2015.march.30.16

Cited by 15 publications

(12 citation statements)

References 33 publications

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“…These factors make sperm very sensitive and vulnerable to the attack of free radicals and initiation of LPO cascades (Bansal and Bilaspuri, 2011). In addition, over-production of ROS and free radicals induced during freeze-thawed semen process has harmful effects on sperm quality (Maia et al, 2010;Zhao et al, 2015). MDA, an oxidizing agent, was measured to determine the oxidative stress and LPO in various cell types (Urso and Clarkson, 2003).…”

Section: Discussionmentioning

confidence: 99%

Effect of Melatonin Supplementation to Freezing Extender on Post-Thaw Sperm Kinetics, Antioxidant Capacity and Fertility Traits in Buck Rabbits

Dessouki

Sakr

2019

EPSJ

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This study was carried out to monitor the fertilizing ability of rabbit semen frozen in the presence of melatonin as antioxidant. Semen from 10 mature Egyptian Baladi red bucks was pooled, extended with Tris-glucose-citric extender (1:1 v/v) supplemented by three concentrations of melatonin (10 -9 , 10 -6 and 10 -3 M) in addition to control, and finally cryopreserved at -196°C. After thawing, sperm kinetics, antioxidant capacity and fertility traits were evaluated. The results showed that total and progressive motility percentages were higher significantly in samples cryopreserved by melatonin (10 -6 M) as compared to control group. Curvilinear and average path sperm velocities were significantly higher in melatonin groups of 10 -9 and 10 -6 M than in the control group. Supplementation of melatonin at 10 -9 M and 10 -6 M groups decreased (P < 0.05) malondialdehyde activity when compared to the control one. In contrast, the total antioxidant capacity and catalase activity were significantly (P < 0.05) elevated at concentration of 10 -6 M melatonin as compared to the control group. In addition, transcript abundance of NFE2L2 and SOD1 genes increased in groups supplemented with melatonin compared to the control group. The fertility trial indicated that pregnancy rate, and the total and live born increased significantly (P < 0.05) in rabbit does inseminated with semen of 10 -6 M melatonin group in compared to the control samples. Conclusively, these results concluded that using melatonin with 10 -6 M level in rabbit freezing extenders could be recommended to improve semen quality and fertilizing ability of buck sperm post-thawing.

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Section: Discussionmentioning

confidence: 99%

Effect of Melatonin Supplementation to Freezing Extender on Post-Thaw Sperm Kinetics, Antioxidant Capacity and Fertility Traits in Buck Rabbits

Dessouki

Sakr

2019

EPSJ

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“…Of the findings in this article, ascorbic acid did not regularly stimulate the activity of bovine liver GR. This result is supported by Zhao et al, who found that the effect of ascorbic acid and vitamin E was not consistent on the antioxidant enzymes in bull plasma [36].…”

Section: Resultsmentioning

confidence: 52%

Investigation of the effects of some water-soluble vitamins on glutathionereductase enzyme purified from bovine liver

Kivanç¹,

Türkoğlu²

2019

Turk J Chem

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Glutathione reductase (GR, EC 1.8.1.7) is an antioxidant enzyme and is involved in the reducing reaction of oxidized glutathione. Glutathione and reduced glutathione are very crucial for micro-and macroorganisms because of their barrier function against radicals. Therefore, the aim of this article was to purify GR and evaluate the relationship between purified GR and vitamins. GR was purified from bovine liver using 2',5'-ADP Sepharose 4B column materials. To check the purity of each subunit of the enzyme, SDS-PAGE electrophoresis was performed and each one was 55 kDa. Afterwards, specific activity and purified ratio for the enzyme were calculated as 54 EU/mg × protein and 2700 times, respectively. Vitamins have a regulatory role in organisms and also some, like vitamin B, are coenzymes due to having cofactor effects. In this project, variable concentrations of some water-soluble vitamins, riboflavin, ascorbic acid, nicotinamide, folic acid, and thiamine, were tested. As a result of these kinetic findings, nicotinamide and folic acid increased the activity of glutathione reductase, while thiamine decreased it. Riboflavin and ascorbic acid did not show a stable effect on enzyme activity. Ki and IC 50 constants were found as 50.16 ± 5.63 mM and 21.04 mM, respectively, when Lineweaver-Burk and activity % vs. inhibitor concentration graphs were drawn for thiamine. The results of this article illustrate that the behaviors of vitamins could be complicated and vary from one living organism to other; in other words, they are unpredictable.

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“…However, the protective effects exerted by MT on cardiomyocytes under conditions of HS remain to be elucidated. It is widely accepted that vitamin E (VE) is an effective antioxidant (13). In the present study, we hypothesized that VE may exert a synergistic effect with MT, both of which participate in the maintenance of mitochondrial function and ROS levels under conditions of HS.…”

Section: Introductionmentioning

confidence: 93%

Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein

Wang

Dong

Yuan

et al. 2016

International Journal of Molecular Medicine

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Heat stress (HS) is commonly used to refer to the heat load that an individual is subjected to due to either metabolic heat, or environmental factors, including high temperatures and high humidity levels. HS has been reported to affect and even damage the functioning of various organs; overexposure to high temperatures and high humidity may lead to accidental deaths. It has been suggested that the cardiovascular system is primarily targeted by exposure to HS conditions; the HS-induced dysfunction of cardiomyocytes, which is characterized by mitochondrial dysfunction, may result in the development of cardiovascular diseases. The excessive production of reactive oxygen species (ROS) also participates in mitochondrial dysfunction. However, effective methods for the prevention and treatment of mitochondrial and cardiovascular dysfunction induced by exposure to HS are lacking. In the present study, we hypothesized that vitamin E (VE), an antioxidant, is capable of preventing oxidative stress and mitochondrial injury in cardiomyocytes induced by exposure to HS. The results revealed that pre‑treatment with VE increased the expression of metallothionein (MT), which has previously been reported to confer cytoprotective effects, particularly on the cardiovascular system. Pre-treatment with VE restored mitochondrial function in cardiomyocytes under conditions of HS by increasing the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM), and by increasing adenosine triphosphate (ATP) levels. Furthermore, pre-treatment with VE decreased the production of ROS, which was induced by exposure to HS and thus exerted antioxidant effects. In addition, pre-treatment with VE attenuated oxidative stress induced by exposure to HS, as demonstrated by the increased levels of antioxidant enzymes [superoxide dismutase (SOD) and glutathione (GSH)], and by the decreased levels of markers of oxidative injury [malondialdehyde (MDA) and lactate dehydrogenase (LDH)]. Taken together, these findings suggest that pre-treatment with VE can prevent mitochondrial dysfunction and oxidative stress in cardiomyocytes induced by exposure to HS, by increasing the expression of MT.

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Protective effects of ascorbic acid and vitamin E on antioxidant enzyme activity of freeze-thawed semen of Qinchuan bulls

Cited by 15 publications

References 33 publications

Effect of Melatonin Supplementation to Freezing Extender on Post-Thaw Sperm Kinetics, Antioxidant Capacity and Fertility Traits in Buck Rabbits

Effect of Melatonin Supplementation to Freezing Extender on Post-Thaw Sperm Kinetics, Antioxidant Capacity and Fertility Traits in Buck Rabbits

Investigation of the effects of some water-soluble vitamins on glutathionereductase enzyme purified from bovine liver

Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein

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