Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes

Saitoh, Yasukazu; Yamaguchi, Yuuki; Okada, Yuhei

doi:10.1007/s11010-021-04189-z

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…In the current study, however, it is first time for molecular hydrogen to be reported to play a protective role against acute sperm damage caused by EMP exposure, which is in accordance with our previous work that molecular hydrogen can alleviate the ionizing radiation injuries by selectively scavenging reactive oxygen species [20,22,45,46]. Moreover, our findings are also consistent with those of others that molecular hydrogen can also mitigate oxidative damages induced by other factors such as hydrogen peroxide, greatly expanding its potential application scope [47].…”

Section: Discussionsupporting

confidence: 92%

Molecular Hydrogen Reduces Electromagnetic Pulse-Induced Male Rat Reproductive System Damage in a Rodent Model

Hao

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Infertility has got to be a broadly concerned social issue these days, in which the malefactor cannot be overlooked. Numerous studies have shown that electromagnetic pulse (EMP) radiation may have seriously damaging effects on reproductive health, through nonthermal effects and oxidative stress. Molecular hydrogen, a selective hydroxyl radical scavenger, explains the protective effects against many diseases closely associated with oxidative damage, such as ionizing radiation (IR). We sought to characterize the beneficial effects of molecular hydrogen on the male reproductive system in a rodent EMP exposure model. The 8-week-old male Sprague-Dawley rats were exposed to EMP (peak intensity 1000 kV/m, pulse edge 20 ns, pulse width 200 ns, 1 Hz, and 200 pulses), with or without hydrogen-rich water. The pathological structure of the testis, the rate of apoptosis of the testis, the serum testosterone level, the sperm parameters, and the activity of the antioxidant enzymes of the testis were measured. Then, transcriptomic and untargeted metabolomic analyses were applied to uncover the underlying mechanism. Exposure to EMP increased testicular apoptosis rate and apoptosis protein level, decreased sperm viability and motility, decreased serum testosterone levels, and diminished testicular antioxidant capacity. Molecular hydrogen-alleviated damage decreased the testicular apoptosis rate and apoptosis protein level, increased sperm motility, increased serum testosterone levels, and improved antioxidative capacity. Omics results showed that molecular hydrogen has a strong influence on metabolic pathways, and EMP affects mainly oxidative phosphorylation, TNF signaling pathways, and cytokine-receptor interactions. The mechanism of molecular hydrogen’s effect may be related to the reversal of some metabolite levels. These observations warrant molecular hydrogen as an innovative approach for potential protection against EMP.

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Section: Discussionsupporting

confidence: 92%

Molecular Hydrogen Reduces Electromagnetic Pulse-Induced Male Rat Reproductive System Damage in a Rodent Model

Hao

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Recently, various research studies on the use of hydrogen molecules for healthcare have also been vigorously advancing [11][12][13]. Hydrogen molecules have also recently attracted attention as new antioxidants and are expected to become new gaseous therapeutic and prophylactic agents related to various kinds of disorders [11][12][13], such as oxidative-stress-induced cellular injuries [4,[14][15][16], wound repair [14,17], blood fluidity [18][19][20], ischemia-reperfusion injuries [21,22], inflammation [23,24], lipid metabolism [25,26], type 2 diabetes [27], neurodegenerative diseases [28], and cancer [29].…”

Section: Introductionmentioning

confidence: 99%

“…More recently, a home-use electrolysis-type hydrogen molecule generator has been developed, which can produce HRW-containing nano-bubbles and make it possible to routinely carry out hydrogen molecule intake at home within one's usual lifestyle for treatment [26,[32][33][34]. It is expected to function in the prevention of oxidative-stress-related modulations and/or diseases [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. The combination of a home-use electrolysis-type hydrogen molecule generator with bathing is expected to provide a more efficient approach for the skin.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen-Nano-Bubble-Rich Water in Bucket/Bathtub Improves Intractable Skin Roughness

Saitoh

Takada²,

Miwa

2023

Hydrogen

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Determining how to apply hydrogen as a therapeutic/preventive antioxidant for oxidative-stress-related diseases practically in daily life has not been studied. The effects of bathtubs and buckets filled with hydrogen water (41 °C, >10 min bathing) were investigated on six subjects, without a medical prescription, suffering from skin roughness on the foot, hand, finger, or elbow. They were also treated with an electrolyzer composed of a lattice-shaped, microscopically flat, platinum-plated three-layer electrode, except for one subject who was treated with a micro-porous emittance terminal hydrogen-jetting apparatus, resulting in improvements in both cases. For another subject with similar skin roughness on both hands, immersing the right hand in an electrolytically generated hydrogen water bucket showed more marked improvement than immersing the left hand in a bucket with normal water. The nano-bubbles (average, mode, and median sizes of 157 nm, 136 nm, and 94 nm, respectively) increased 3.79 fold to 2.20 × 108/mL after 30 min electrolysis with 2 L of tap water and were boiling (98 °C, 2 min)-resistant, with heat stability in nano-bubbles as small as 69–101 nm, as evaluated by laser-beam-based Brownian movement trailing Nano-Sight analysis. The marked increase in nano-bubbles caused by electrolysis correlated with an increase in dissolved hydrogen (<15 μg/L to 527 μg/L) but not a decrease in dissolved oxygen (9.45 mg/L to 6.94 mg/L). Thus, the present study proposed the novelty of hydrogen regarding its contribution to health from the perspective that hydrogen-nano-bubble-rich water in a foot bucket, which was additively used together with a conventional bathtub and can be frequently used in daily life, improved diverse types of skin roughness.

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Molecular hydrogen suppresses Porphyromonas gingivalis lipopolysaccharide-induced increases in interleukin-1 alpha and interleukin-6 secretion in human gingival cells

Saitoh

Yonekura

Matsuoka³

et al. 2021

Mol Cell Biochem

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Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes

Cited by 6 publications

References 53 publications

Molecular Hydrogen Reduces Electromagnetic Pulse-Induced Male Rat Reproductive System Damage in a Rodent Model

Molecular Hydrogen Reduces Electromagnetic Pulse-Induced Male Rat Reproductive System Damage in a Rodent Model

Hydrogen-Nano-Bubble-Rich Water in Bucket/Bathtub Improves Intractable Skin Roughness

Molecular hydrogen suppresses Porphyromonas gingivalis lipopolysaccharide-induced increases in interleukin-1 alpha and interleukin-6 secretion in human gingival cells

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