Protective Effects of Phellinus linteus Mycelium on the Development of Osteoarthritis after Monosodium Iodoacetate Injection

Abstract

Objective. The aim of this study was to identify the protective effects of Phellinus linteus mycelium (PLM) and its possible mechanisms in a model of monosodium iodoacetate- (MIA-) induced osteoarthritis (OA). Methods. Intra-articular injection of MIA was injected to 50 μL with 80 mg/mL using a 0.3 mL insulin syringe into the right knee joint. Changes in hindpaw weight-bearing distribution between the right (osteoarthritic) and left (contralateral control) legs were used as an index of joint discomfort. PLM (50, 100, and 200 mg/kg body weight) was orally administered once daily for 14 days from day 7 after MIA treatment. And then, various factors associated with inflammatory response and cartilage degeneration in cartilage tissues detected by western blotting. Results. PLM treatment showed a concentration-dependent elevation in change in hindpaw weight-bearing distribution (HWBD). PLM200 demonstrated the capacity to significantly increase HWBD, indicating that the change in weight-bearing distribution means the reduction of spontaneous pain. Our results indicate that PLM suppressed the inflammatory factors via NF-κB signaling pathway induced by p38 phosporlyation. Moreover, PLM200 exhibited a significant reduction of ROS produced by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. PLM100 and PLM200 inhibited the levels of matrix metalloproteinase (MMP)-1, one of proteinase that degrades extracellular matrix (ECM). Conclusions. Taken together, our results indicated that PLM has a strong chondroprotective effect through the suppression both ROS production and inflammation.

Effects of Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate on Grip Strength, Cartilage Structure and Joint Remodelling Markers in a Rat Model of Osteoarthritis

“…In another study, MIA arrested chondrocyte proliferation due to S-phase arrest [28]. MIA has been commonly used to induce cartilage damage in animals to mimic osteoarthritis in humans [29][30][31]. In this study, MIA induced apparent cartilage damage, as similarly observed in previous studies [19,32].…”

Anti-Inflammatory and Analgesic Effects of Schisandra chinensis Leaf Extracts and Monosodium Iodoacetate-Induced Osteoarthritis in Rats and Acetic Acid-Induced Writhing in Mice

“…IL-1β activates transcription factors via the MAPK and NF-κB signalling pathways to regulate inflammatory responses, resulting in the production of inflammatory mediators such as NO, PGE2, COX-2, and other catabolic factors that may accelerate cartilage degeneration [ 30 , 31 , 32 ]. Moreover, cytokines upregulate the gene expression of MMPs, which promotes the degradation of articular cartilage by breaking down proteoglycan, the main component of the ECM [ 33 , 34 ]. MMP-2 and MMP-9 are gelatinases that degrade a broad range of collagen and proteoglycans.…”

Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis

“…Administration. A rat model of OA was induced by MIA, as previously described [24]. Seven-week-old male Sprague-Dawley rats (170-200 g; DBL Co., Eumseong, Korea) were housed three per cage with free access to food (ENVIGO, USA) and water, in a room with controlled temperature (22 ± 2 °C), humidity (55 ± 5%), and lighting (12 h light/dark cycle, 200-300 Lux).…”