Protective effects of plasma replacement fluids on erythrocytes exposed to mechanical stress

Sümpelmann, Robert; Schürholz, Tobias; Marx, Gernot; Zander, R.

doi:10.1046/j.1365-2044.2000.01531.x

Cited by 10 publications

(8 citation statements)

References 11 publications

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“…5b). Results in this direction have also been noted by previous researchers (Sumpelmann et al 2000;Hughes et al 1986). Exposing a suspension of human RBCs in a gelatin and albumin fluid, instead of 0.9% (154 mM) NaCl, to mechanical stress, showed increased stability of the RBCs (Sumpelmann et al 2000).…”

Section: Potential Reasons For the Exponential Lysissupporting

confidence: 81%

Improving the Stability of Red Blood Cells in Rainbow Trout (Oncorhynchus mykiss) and Herring (Clupea harengus): Potential Solutions for Post-mortem Fish Handling to Minimize Lipid Oxidation

Ghirmai

Eriksson

et al. 2020

Food Bioprocess Technol

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This study aimed at limiting hemolysis of fish red blood cells (RBCs) as a strategy to limit hemoglobin (Hb)-induced lipid oxidation during post-mortem handling and processing. Effects of varying temperature, salinity, and mechanical impact were studied using washed resuspended RBCs (wr-RBCs) and whole blood (WB) from rainbow trout (Oncorhynchus mykiss) and herring (Clupea harengus). The wr-RBCs were most stable avoiding mechanical stress, keeping isotonic conditions (0.9-1.3% NaCl) and low temperature 0-6°C, with predicted minimum at 2.5°C. When compared at the same salinity, it was found that hemolysis was more pronounced in herring than trout wr-RBCs. Furthermore, WB was more stable than wr-RBCs, showing protecting the effects of blood plasma. Studying individual plasma components, stabilizing effects were found from glucose, proteins, and ascorbic acid. This study indicates that small adjustments in the early handling and processing of fish such as changing salinity of storage and rinsing solutions could minimize Hb contamination of the fish muscle and thereby improve quality.

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Section: Potential Reasons For the Exponential Lysissupporting

confidence: 81%

Improving the Stability of Red Blood Cells in Rainbow Trout (Oncorhynchus mykiss) and Herring (Clupea harengus): Potential Solutions for Post-mortem Fish Handling to Minimize Lipid Oxidation

Ghirmai

Eriksson

et al. 2020

Food Bioprocess Technol

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“…Note that washed RBCs resuspended in PBS were injected into the free jet of PBS. According to Kameneva et al 27 and Sumpelmann et al 28 , the fragility of RBCs increases approximately by 3 – 4 times when RBCs are suspended in saline as opposed to plasma, thus, the critical value of RSS found in the present study may increase by the same factor if it were suspended in plasma. Nevertheless, an order of magnitude underestimation of the critical RSS suggested by Sallam 7 is still noticeable from this comparative study.…”

Section: Discussionsupporting

confidence: 52%

Determination of Reynolds Shear Stress Level for Hemolysis

et al. 2018

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Reynolds shear stress (RSS) has served as a metric for the effect of turbulence on hemolysis. Forstrom (1969) and Sallam and Hwang (1984) determined the RSS threshold for hemolysis to be 50,000 and 4,000 dyne/cm, respectively, using a turbulent jet. Despite the order of magnitude discrepancy, the threshold by Sallam and Hwang has been frequently cited for hemolytic potential in blood pumps. We recreated a Sallam apparatus (SA) to resolve this discrepancy and provide additional data to be used in developing a more accurate hemolysis model. Hemolysis was measured over a large range of Reynolds numbers (Re) (Re = 1,000-80,000). Washed bovine red blood cells (RBCs) were injected into the free jet of phosphate buffered saline, and hemolysis was quantified using a percent hemolysis, Hp = h (100 - hematocrit [HCT])/Hb, where h (mg/dl) is free hemoglobin and Hb (mg/dl) is total hemoglobin. Reynolds shear stress was calculated using two-dimensional laser Doppler velocimetry. Reynolds shear stress of ≥30,000 dyne/cm corresponding to Re of ≥60,000 appeared to cause hemolysis (p < 0.05). This RSS is an order of magnitude greater than the RSS threshold that Sallam and Hwang suggested, and it is similar to Forstrom's RSS threshold. This study resolved a long-standing uncertainty regarding the critical values of RSS for hemolysis and may provide a foundation for a more accurate hemolysis model.

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“…The clinical significance of these results is that a decrease in the concentration of plasma proteins due to hemodilution may elevate blood damage during extracorporeal circulation (64). Protective effects of a 4%-modified fluid gelatin solution and a 4% albumin solution on blood bank-stored RBC exposed to mechanical stress has been reported by Sumpelmann et al (71). A similar protective effect was demonstrated by replacement of 20% of blood plasma with a perfluorochemical-based blood substitute in in vitro experiments, promising improvement in blood oxygenation as well (65).…”

Section: Protection Of Red Blood Cells From Mechanical Traumamentioning

confidence: 88%

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

et al. 2016

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After many decades of improvements in mechanical circulatory assist devices (CADs), blood damage remains a serious problem during support contributing to variety of adverse events, and consequently affecting patient survival and quality of life. The mechanisms of cumulative cell damage in continuous-flow blood pumps are still not fully understood despite numerous in vitro, in vivo, and in silico studies of blood trauma. Previous investigations have almost exclusively focused on lethal blood damage, namely hemolysis, which is typically negligible during normal operation of current generation CADs. The measurement of plasma free hemoglobin (plfHb) concentration to characterize hemolysis is straightforward, however sublethal trauma is more difficult to detect and quantify since no simple direct test exists. Similarly, while multiple studies have focused on thrombosis within blood pumps and accessories, sublethal blood trauma and its sequelae have yet to be adequately documented or characterized. This review summarizes the current understanding of sublethal trauma to red blood cells (RBCs) produced by exposure of blood to flow parameters and conditions similar to those within CADs. It also suggests potential strategies to reduce and/or prevent RBC sublethal damage in a clinically-relevant context, and encourages new research into this relatively uncharted territory.

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Protective effects of plasma replacement fluids on erythrocytes exposed to mechanical stress

Cited by 10 publications

References 11 publications

Improving the Stability of Red Blood Cells in Rainbow Trout (Oncorhynchus mykiss) and Herring (Clupea harengus): Potential Solutions for Post-mortem Fish Handling to Minimize Lipid Oxidation

Improving the Stability of Red Blood Cells in Rainbow Trout (Oncorhynchus mykiss) and Herring (Clupea harengus): Potential Solutions for Post-mortem Fish Handling to Minimize Lipid Oxidation

Determination of Reynolds Shear Stress Level for Hemolysis

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

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