Protective effects of seaweed supplemented diet on antioxidant and immune responses in European seabass (Dicentrarchus labrax) subjected to bacterial infection

Peixoto, Maria João; Ferraz, Renato; Magnoni, Leonardo J.; Pereira, Rui; Gonçalves, José F.; Calduch-Giner, Josep Àlvar; Pérez‐Sánchez, Jaume; Ozório, Rodrigo O. A.

doi:10.1038/s41598-019-52693-6

Cited by 41 publications

(18 citation statements)

References 66 publications

(64 reference statements)

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“…Extracts from berries (rich in quercetin and catechins) like murtilla [ 141 ] and blueberries proved to inhibit sperm lipid peroxidation in Arctic char [ 6 ], while sesame seed powder diet, containing lignans like sesamin, was able to improve sperm motility duration, hatchability, and egg survival rate in African catfish [ 142 ]. Even algae extracts of Gracilaria sp., containing phenolic compounds, proved to have an antioxidant effect on European seabass challenged with a bacterial infection, decreasing lipid peroxidation and suggesting higher total antioxidant capacity in fish fed with experimental diet [ 143 ]. Other polyphenolic substances that have been widely used in many different types of antioxidant assays are honeybee products such as honey, propolis, and royal jelly.…”

Section: Fish Sperm Antioxidant System and Supplementation: Classimentioning

confidence: 99%

Antioxidants in Fish Sperm and the Potential Role of Melatonin

2020

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In recent years, the effects of novel antioxidants have played an important role in the research focusing on fish cell protection. As food demand grows, aquaculture production becomes more intensive, and fish are more exposed to oxidative stress conditions, like high densities, temperature shifting, frequent fish handling and samplings, and prophylactic or disease treatments, which expose fish to a different environment. Particularly in reproduction, germ cells lose antioxidant capacity with spermatogenesis, as spermatozoa are more prone to oxidative stress. Antioxidants have been used in a variety of fish physiological problems including in reproduction and in the establishment of cryopreservation protocols. From the most used antioxidants to natural plant food and herbs, and endogenously produced antioxidants, like melatonin, a review of the literature available in terms of their effects on the protection of fish spermatozoa is presented here in a classified structure. Several direct and indirect approaches to improve gamete quality using antioxidants administration are mentioned (through feed supplementation or by adding in cryopreservation media), as well as factors affecting the efficiency of these molecules and their mechanisms of action. Special attention is given to the unclear melatonin pathway and its potential scavenger activity to prevent and counteract oxidative stress damage on fish spermatozoa.

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Section: Fish Sperm Antioxidant System and Supplementation: Classimentioning

confidence: 99%

Antioxidants in Fish Sperm and the Potential Role of Melatonin

2020

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Section: Discussionmentioning

confidence: 99%

“…While there are some studies demonstrating antioxidant benefits of seaweed supplements in fish and aquaculture (e.g. reducing by half lipid peroxidation; Wan et al 2019;Peixoto et al 2019;Kamunde et al 2019), the potential for seaweeds to reduce oxidative stress in fish remains largely unknown (Peixoto et al 2016a). These and other novel applications of seaweed in animal health include potential benefits for humans; for example, the antiviral activity of fucoidan from the brown seaweed Saccharina japonica against the SARS-CoV-2 virus was substantially higher than Remdesivir (GS-5734), which, at the time of writing was approved for emergency treatment of severe COVID-19 cases (Kwon et al 2020).…”

Section: Potential Mechanisms Of Seaweed Immunostimulantsmentioning

confidence: 99%

“…There is also much variation in the ways in which researchers deliver seaweeds to fish in experiments, with some using whole seaweeds (Satoh et al 1987) and others using various extracts (e.g. methanol; Peixoto et al 2019). Moreover, many different seaweed species have been trialled for various freshwater and marine fish species, using different methods and modes of delivery, different immune parameters have been measured and trials have been run under many different experimental conditions (Díaz‐Rosales et al 2005; de las Heras et al 2010; Cheng et al 2012; Kim et al 2014), making the merits of direct comparisons between individual studies questionable.…”

Section: Introductionmentioning

confidence: 99%

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Meta‐analysis of the use of seaweeds and their extracts as immunostimulants for fish: a systematic review

Thépot

Campbell

Rimmer

et al. 2020

Reviews in Aquaculture

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Disease is an enduring threat to aquaculture, with direct costs from outbreaks of more than US$6 billion a year. Efforts to reduce antibiotic use in aquaculture have resulted in a recent shift towards the use of immunostimulants that boost fish immune systems. Seaweeds are a potential source of unique immunostimulants because of their diverse taxonomy (11,353 species) and diverse bioactives, but there has been no synthesis of their effects on fish. A systematic review and meta‐analysis of 142 peer‐reviewed studies was conducted to evaluate the effects of seaweed on the innate immunity, resistance (to stress and pathogens) and growth of farmed fish. Overall, our review indicated that dietary supplementation with seaweeds or their extracts had substantial positive impacts on the health and growth of fish compared with control groups and were comparable to currently utilised immunostimulants. Dramatic effects on survival of fish during challenge experiments were detected, with an average of 33% higher survival of fish fed diets that included seaweeds or their extracts. This study provides broad evidence for the benefits of supplementing fish feed with the reviewed seaweeds or their bioactive extracts, including potential synergistic effects of supplements. However, the meta‐analysis also highlighted considerable variation among studies, and between fish and seaweed species, making it difficult to extrapolate results beyond individual experiments. Future studies should focus on developing synergistic combinations of ingredients and elucidating the mode of action through isolation of seaweed bioactives, together with standardisation of experimental conditions, to fast‐track the benefits of seaweed supplements for aquaculture.

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“…However, P. damselae subsp. piscicida caused a down‐regulation in the hepatic antioxidant genes of European seabass ( Dicentrarchus labrax ), and there is no a significant difference between the control and infected group in serum samples (Peixoto et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

El‐Son

Elbahnaswy

Ibrahim

2020

Journal of Fish Diseases

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Mass mortality has occurred among cultured Nile tilapia, Oreochromis niloticus, on fish farms in Manzala, Dakahlia province, Egypt, in the summer season, 2019. Moribund fish were reported with deep ulcers, septicaemic lesions and sampled for bacterial isolation. In this study, most isolates were subjected to bacteriological examination, antibiotic sensitivity test, 16S rRNA gene sequencing and histopathological examination. Following isolate identification, intraperitoneal challenge of Nile tilapia with a bacterial suspension 2 × 106 CFU/ml was performed. Samples from liver, spleen and kidney were collected for histological and biochemical analysis. The results showed a high similarity (99%) to Photobacterium damselae strains using phylogenetic analysis of 16S rRNA. P. damselae exhibited resistance to amoxicillin and erythromycin, as well it was highly sensitive to chloramphenicol and doxycycline. Moreover, haemorrhage, oedema, hemosiderosis and melanomacrophage activation in the liver and head kidney of infected fish were detected by light and electron microscopy. Also, significant higher levels of CAT and SOD in the spleen and head kidney, as well as the serum levels of NO were observed in experimentally challenged O. niloticus, compared to the control fish. Our data identified P. damselae for the first time from infected Nile tilapia, describing its sensitivity to a variety of antibiotics, histopathological alterations and oxidative stress impact, and it could be useful indicators for understanding P. damselae pathogenesis, which might provide a preventive efficacy for P. damselae.

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Protective effects of seaweed supplemented diet on antioxidant and immune responses in European seabass (Dicentrarchus labrax) subjected to bacterial infection

Cited by 41 publications

References 66 publications

Antioxidants in Fish Sperm and the Potential Role of Melatonin

Antioxidants in Fish Sperm and the Potential Role of Melatonin

Meta‐analysis of the use of seaweeds and their extracts as immunostimulants for fish: a systematic review

Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

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