Protective Effects on 60Co-γ Radiation Damage of Pine Cone Polyphenols from Pinus koraiensis-Loaded Chitosan Microspheres In Vivo

Abstract

A novel chitosan microsphere for encapsulating pine cone polyphenols (PP) from P. koraiensis was successfully prepared using an emulsion crosslinking technique. The characteristics of pine polyphenol-loaded microspheres (PPM) were determined using scanning electron microscopy (SEM) and a laser particle size detector. It was found that PPMs were spherical in shape with uniform particle size distribution patterns. The drug content and encapsulation rate of the microspheres were 7.47% and 73.6%, respectively, at a Ch/GA mass ratio of 0.7. The animal experiments showed that PPM had a stronger radiation protective effect than PP. PPM significantly increased the immune organ indices, the quantity of marrow DNA, the superoxide dismutase (SOD) activity, the splenocyte proliferation index, and the phagocytosis activity of monocytes. PPM also decreased the numbers of micronuclei in bone marrow cells and malondialdehyde (MDA) levels in plasma in mice exposed to 60Co γ-irradiation. In addition, gender differences in biological responses to exposure to radiation were observed.

Protective effect of coix seed seedling extract on 60Co‐γ radiation‐induced oxidative stress in mice

“…The hematopoietic system is sensitive to radiation (Kim et al., 2019). The decrease of the number of WBC in the peripheral blood and the contents of DNA in bone marrow can reflect the damage degree of hematopoietic system (Shao et al., 2018). As presented in Figure 3a, compared with NC group (5.11 × 10 3 cells/µl), the number of WBC in IR group (0.96 × 10 3 cells/µl) was significantly reduced by 81.21% after 60 Co‐γ radiation ( p < 0.0001), indicating that IR could cause great damage to WBC of mice and lead to the weakened hematopoietic function (Amini et al., 2020).…”

Identification and Validation of a Prognostic Risk-Scoring Model Based on Ferroptosis-Associated Cluster in Acute Myeloid Leukemia

“…Resting mast cells are immunoreactive cells that are related to better survival (de Alencar et al, 2020). In opposition, monocytes play an important role in tumor growth and progression (Shao et al, 2018). Therefore, a negative correlation of risk score with naïve B cells, naive CD4 + T cells, and resting mast cells and the positive correlation with monocytes suggests that the 18-gene signature is tightly associated with immune-active status in the tumor microenvironment.…”

Preliminary study on the anti-apoptotic mechanism of Astragaloside IV on radiation-induced brain cells

“…After 1 month of administration, 60 Co radiation (Lanzhou Weite radiation Co., LTD, Lanzhou, China) was used in vivo, and the cumulative radiation dose was 8 Gy. 13 – 15 …”

“…After attaching to 70%–80% of the wall, the cells were irradiated at a vertical distance of 15 cm from the UVA light as radiation source (the cell radiation dose was about 6.5 J/cm 2 ), the irradiation time was 45 min, then treated with the drugs. 13 , 20 – 22 …”

“…After 1 month of administration, 60 Co radiation (Lanzhou Weite radiation Co., LTD, Lanzhou, China) was used in vivo, and the cumulative radiation dose was 8 Gy. [13][14][15] Cell culture PC12 cells were derived from Lanzhou University School of Basic Medicine and cultured in a humidified incubator containing 5% CO 2 in high glucose medium (Cell Max, Hyclone, Logan City, UT, USA). The high glucose medium contained 5% fetal bovine serum (Gibco, Grand Island, NY, USA), 10% horse serum (Gibco), and 100 U/mL of penicillin with 100 mg/mL of streptomycin (Hyclone).…”