Protective immunity against nervous necrosis virus in convict grouper Epinephelus septemfasciatus following vaccination with virus-like particles produced in yeast Saccharomyces cerevisiae

Wi, Ga Ram; Hwang, Jee Youn; Kwon, Mun‐Gyeong; Kim, Hyoung Jin; Kang, Hyun Ah; Kim, Hong-Jin

doi:10.1016/j.vetmic.2015.02.021

Cited by 31 publications

(18 citation statements)

References 23 publications

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“…The expression of the viral protein as virus-like particles (VLPs) has also been achieved in the yeast Yarrowia lipolytica [337]. The efficacy of recombinant proteins or VLPs has also been assessed in other studies [338][339][340][341][342][343][344] and the stability of the VLP vaccine after lyophilization has been demonstrated [345].…”

Section: Preventionmentioning

confidence: 99%

“…Protection conferred by recombinant-protein vaccines has been reported to be high, i.e., vaccinated giant grouper RPS values above 72% [336] and increasing up to more than 80% in Atlantic sea bass [340]. Recently, total protection has been claimed in vaccinated convict/seven band grouper and European sea bass [335,341].…”

Section: Preventionmentioning

confidence: 99%

“…injection [289,328,329,331,332,349]. Injection has also been used for DNA vaccines [347,349] and subunit vaccines [335,336,338,[340][341][342] and oral immunization strategies have been used either for inactivated, subunit or DNA vaccines [225,[333][334][335]341,343,348,351,352]. The oral vaccine was given to the fish mixed with food [343] or by oral gavage [333,341] and encapsulated using either chemical compounds [225,348] or Artemia [353].…”

Section: Preventionmentioning

confidence: 99%

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Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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Section: Preventionmentioning

confidence: 99%

Section: Preventionmentioning

confidence: 99%

Section: Preventionmentioning

confidence: 99%

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Betanodavirus and VER Disease: A 30-year Research Review

2020

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“…On the other hand, published data on oral immunization of VNN and neutralizing antibody production are very limited. Wi et al (2015) reported that a Fish (45 g in mean body weight) fed inactivated RGNNV vaccine-feed with/without capsaicin for consecutive seven days. At 21 day post-immunization, fish (recipient) were exposed to drainage from an tank of fish (donor) previously infected with RGNNV and observed for 25 days.…”

Section: Resultsmentioning

confidence: 99%

An Oral Vaccination Method with the Aid of Capsaicin against Viral Nervous Necrosis (VNN)

et al. 2018

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“…Very promising results on oral vaccination against VER have been reported using VLPs. In convict grouper (Epinephelus septemfasciatus), Wi et al [46] demonstrated 100% survival with i.p. injection of RGNNV VLPs produced in yeast (Saccharomyces cerevisiae) against an RGNNV challenge and 57% survival when the VLPs were orally administered [46].…”

Section: Discussionmentioning

confidence: 99%

Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole

Thwaite

Berbel

Aparicio

et al. 2020

Fish & Shellfish Immunology

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Nervous necrosis virus (NNV) reassortant strains RGNNV/SJNNV have emerged as a potent threat to the Mediterranean marine aquaculture industry, causing viral encephalopathy and retinopathy (VER) in Senegalese sole (Solea senegalensis). In this study, a cheap and practical vaccine strategy using bacterial inclusion bodies made of the coat protein of a virulent reassortant strain of this betanodavirus was devised. The nanostructured recombinant protein nanoparticles, VNNV-C NP , were administered without adjuvant to two groups of juvenile sole, one by intraperitoneal injection and the other by oral intubation. Specific antibodies were raised in vivo against the NNV coat protein via both routes, with a substantial specific antibody expansion in the injected group 30 days post homologous prime boost. Expression levels of five adaptive immune-related genes, cd8a, cd4, igm, igt and arg2, were also quantified in intestine, spleen and head kidney. Results showed cd4 and igm were upregulated in the head kidney of injected fish, indicating activation of an adaptive systemic response, while intubated fish exhibited a mucosal response in the intestine. Neither route showed significant differential expression of cd8a. The specific antibody response elicited in vivo and the lack of any signs of toxicity over the 6-week study period in young fish (n=100), evidences the potential of the nanoparticle as a vaccine candidate.

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Protective immunity against nervous necrosis virus in convict grouper Epinephelus septemfasciatus following vaccination with virus-like particles produced in yeast Saccharomyces cerevisiae

Cited by 31 publications

References 23 publications

Betanodavirus and VER Disease: A 30-year Research Review

Betanodavirus and VER Disease: A 30-year Research Review

An Oral Vaccination Method with the Aid of Capsaicin against Viral Nervous Necrosis (VNN)

Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole

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