Protective immunity induced by concurrent intradermal injection of porcine circovirus type 2 and Mycoplasma hyopneumoniae inactivated vaccines in pigs

Lee, Sim-In; Jeong, Chang-Gi; Mattoo, Sameer ul Salam; Nazki, Salik; Aganja, Ram Prasad; Kim, Seung-Chai; Khatun, Amina; Oh, Yeonsu; Noh, Sang-Hyun; Lee, Sang-Myeong; Kim, Won-Il

doi:10.1016/j.vaccine.2021.07.043

Cited by 9 publications

(10 citation statements)

References 60 publications

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“…These pigs were inoculated with the Mhp vaccine just after weaning. Mhp vaccination elicits a weak (and typically negative) antibody response [ 32 ], as in our results. The significantly higher Mhp-specific antibody response on farm B indicates that this response was due to natural infection and reflects the severity of Mhp invasion.…”

Section: Discussionsupporting

confidence: 79%

Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms

Suzuki

Shinkai

Yoshioka

et al. 2022

Animals

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Reduced productivity caused by infections, particularly respiratory diseases, is a serious problem in pig farming. We have previously reported polymorphisms in porcine pattern recognition receptor genes affecting molecular functions and demonstrated that the 2197A/C polymorphism in the nucleotide-binding oligomerization domain containing 2 (NOD2) gene influences porcine circovirus 2-induced mortality. Here, we investigated how these polymorphisms affect respiratory disease-induced lesions, using samples from a slaughterhouse dealing with pigs from two farms. Lung lesions were evaluated using two scoring systems, Goodwin (GW) and slaughterhouse pleuritis evaluation system (SPES), to determine the influence of Mycoplasma hyopneumoniae (Mhp) and Actinobacillus pleuropneumoniae (App), respectively. SPES scores were significantly higher when the 1205T allele of Toll-like receptor 5 (TLR5-1205T), rather than TLR5-1205C, was present. On the farm with more severe Mhp invasion, lower GW lesion scores were significantly associated with the presence of the NOD-like receptor family pyrin domain containing 3 (NLRP3)-2906G allele; where App invasion was worse, lower SPES scores were significantly associated with the presence of the NOD2-2197C allele. Combinations of polymorphisms in pattern recognition receptor genes can therefore be utilized for breeding for resistance against respiratory diseases in pigs. DNA markers of these polymorphisms can thus be used to improve productivity by reducing respiratory diseases due to bacterial pathogens in pig livestock.

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Section: Discussionsupporting

confidence: 79%

Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms

Suzuki

Shinkai

Yoshioka

et al. 2022

Animals

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“…In a few studies, minipigs were used as they are regarded as a suitable model when it comes to vaccination through the skin [ 83 ]. It is noted that almost half of the identified papers (n = 57), do not name the pig breed, often referring to it only as commercial breed [ 49 , 51 , 76 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 ,…”

Section: Resultsmentioning

confidence: 99%

“…Adjuvants are used to enhance and modulate vaccine-induced immunity. Over 60% of identified papers using skin-based vaccination in pigs included adjuvants; the majority mixed or co-inoculated with the vaccine [ 51 , 76 , 88 , 108 , 110 , 112 , 113 , 115 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 129 , 131 , 132 , 133 , 134 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 146 , 151 , 153 , 154 , 156 , 158 , 159 , 160 , 161 , 168 , 179 , 180 , 182 , 186 , 187 , 188 , 196 , 198 , 199 , 201 , 202 ,…”

Section: Resultsmentioning

confidence: 99%

Skin-Based Vaccination: A Systematic Mapping Review of the Types of Vaccines and Methods Used and Immunity and Protection Elicited in Pigs

2023

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The advantages of skin-based vaccination include induction of strong immunity, dose-sparing, and ease of administration. Several technologies for skin-based immunisation in humans are being developed to maximise these key advantages. This route is more conventionally used in veterinary medicine. Skin-based vaccination of pigs is of high relevance due to their anatomical, physiological, and immunological similarities to humans, as well as being a source of zoonotic diseases and their livestock value. We conducted a systematic mapping review, focusing on vaccine-induced immunity and safety after the skin immunisation of pigs. Veterinary vaccines, specifically anti-viral vaccines, predominated in the literature. The safe and potent skin administration to pigs of adjuvanted vaccines, particularly emulsions, are frequently documented. Multiple methods of skin immunisation exist; however, there is a lack of consistent terminology and accurate descriptions of the route and device. Antibody responses, compared to other immune correlates, are most frequently reported. There is a lack of research on the underlying mechanisms of action and breadth of responses. Nevertheless, encouraging results, both in safety and immunogenicity, were observed after skin vaccination that were often comparable to or superior the intramuscular route. Further research in this area will underlie the development of enhanced skin vaccine strategies for pigs, other animals and humans.

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“…In particular, it was found that in the body of vaccinated pigs, the humoral and cellular immune response factors feature the greatest variability. At the same time, cytotoxic T-cells, T-helper cells of the 1 st (Th1) and 17 th type (Th17) are most strongly activated, reducing the viral load on the body of vaccinated pigs [11]. It was noted in studies [11,12] that vaccination is a stress factor for the animal's body.…”

Section: Introductionmentioning

confidence: 99%

“…At the same time, cytotoxic T-cells, T-helper cells of the 1 st (Th1) and 17 th type (Th17) are most strongly activated, reducing the viral load on the body of vaccinated pigs [11]. It was noted in studies [11,12] that vaccination is a stress factor for the animal's body. Therefore, during the post-vaccination period the changes, typical for alert stage, develop in the body of animals.…”

Section: Introductionmentioning

confidence: 99%

Contribution of some immunological and metabolic factors to formation of piglets’ post-vaccination immunity

Дерхо

Burkov

Scherbakov

et al. 2022

Teor. prakt. pererab. mâsa (Print)

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The role and responsibility of natural resistance factors, protein and lipid metabolism in the formation of piglets post-vaccination immunity against circovirus is researched. Blood was taken for tests before and on the 15th, 40th and 70th day after the vaccination. The sampled blood was analyzed to determine immunological and biochemical parameters. It was revealed that before vaccination, 31.46% of the studied samples have a positive reaction in ELISA; their number increases to 67.80–71.16% on the 40th and 70th days after vaccination.In the blood of piglets, especially on the 40th and 70th day after the vaccination, the total count of leukocytes, monocytes and lymphocytes increases by 1.21; 2.28 times and 1.48 times, but neutrophils reduced by 1.74 times along with the phagocytic properties activation. The anabolic directivity of protein metabolism is defined by the synthesis of globulin proteins. At the same time albumin-synthesizing activity in a liver decreased and “cytolysis reaction” of hepatocytes was detected. In the lipid profile of piglets’ blood, the content of LDL‑cholesterol increased by 1.44 times, while that of triglycerides decreased by 2.64 times. X‑ray spectral analysis revealed the correlation between the formation of post-vaccination immunity and two factors: the factor of the principal component (PC) 1, which is predominantly associated with indicators of natural resistance, and PC2, which is associated with metabolism indicators. The research results show that in order to increase the efficiency of formation of post-vaccination immunity in piglets, it is necessary to combine vaccination with hepatoprotective drugs.

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Protective immunity induced by concurrent intradermal injection of porcine circovirus type 2 and Mycoplasma hyopneumoniae inactivated vaccines in pigs

Cited by 9 publications

References 60 publications

Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms

Polymorphisms in Pattern Recognition Receptor Genes Are Associated with Respiratory Disease Severity in Pig Farms

Skin-Based Vaccination: A Systematic Mapping Review of the Types of Vaccines and Methods Used and Immunity and Protection Elicited in Pigs

Contribution of some immunological and metabolic factors to formation of piglets’ post-vaccination immunity

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