Protective Immunity Induced by TgMIC5 and TgMIC16 DNA Vaccines Against Toxoplasmosis

Zhu, Yuchao; Ma, Lijuan; Zhang, Jili; Liu, Jian-Fa; He, Yong; Feng, Ji-Ye; Chen, Jia

doi:10.3389/fcimb.2021.686004

Cited by 11 publications

(20 citation statements)

References 39 publications

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“…A previous study showed that vaccination with pVAX-MIC16 or pVAX-MIC5 induces T lymphocyte proliferation. In this study, immunization with pVAX-MIC16, pVAX-MIC5 or pVAX-MIC16 + pVAX-MIC15 significantly increased the percentage of CD4 + and CD8 + T cells compared with the control group (Zhu et al, 2021). Furthermore, the proportion of CD4 + and CD8 + T lymphocytes significantly increased in mice vaccinated with the TG290 mRNA-LNP vaccine, consistent with previous studies.…”

Section: Discussionsupporting

confidence: 91%

A novel Toxoplasma gondii TGGT1_316290 mRNA-LNP vaccine elicits protective immune response against toxoplasmosis in mice

Zhang

et al. 2023

Front. Microbiol.

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Toxoplasma gondii (T. gondii) can infect almost all warm-blooded animals and is a major threat to global public health. Currently, there is no effective drug or vaccine for T. gondii. In this study, bioinformatics analysis on B and T cell epitopes revealed that TGGT1_316290 (TG290) had superior effects compared with the surface antigen 1 (SAG1). TG290 mRNA-LNP was constructed through the Lipid Nanoparticle (LNP) technology and intramuscularly injected into the BALB/c mice, and its immunogenicity and efficacy were explored. Analysis of antibodies, cytokines (IFN-γ, IL-12, IL-4, and IL-10), lymphocytes proliferation, cytotoxic T lymphocyte activity, dendritic cell (DC) maturation, as well as CD4+ and CD8+ T lymphocytes revealed that TG290 mRNA-LNP induced humoral and cellular immune responses in vaccinated mice. Furthermore, T-Box 21 (T-bet), nuclear factor kappa B (NF-kB) p65, and interferon regulatory factor 8 (IRF8) subunit were over-expressed in the TG290 mRNA-LNP-immunized group. The survival time of mice injected with TG290 mRNA-LNP was significantly longer (18.7 ± 3 days) compared with the survival of mice of the control groups (p < 0.0001). In addition, adoptive immunization using 300 μl serum and lymphocytes (5*107) of mice immunized with TG290 mRNA-LNP significantly prolonged the survival time of these mice. This study demonstrates that TG290 mRNA-LNP induces specific immune response against T. gondii and may be a potential toxoplasmosis vaccine candidate for this infection.

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Section: Discussionsupporting

confidence: 91%

A novel Toxoplasma gondii TGGT1_316290 mRNA-LNP vaccine elicits protective immune response against toxoplasmosis in mice

Zhang

et al. 2023

Front. Microbiol.

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“…We found that all immunized mice induced significant T. gondii specific splenocyte cell proliferation, suggesting that an adaptive immune response to parasite infection was activated, which may contribute to an effective cellular immune response. 28 the single protein group, the survival time of the rTgBAG1-rTgROP8 group was increased, the brain cysts were reduced, and the humoral and cellular immune responses were enhanced. By further analysis of the ratio of IgG1 to IgG2a of the two IgG subtypes, it was found that the IgG2a level induced by the rTgBAG1 group was higher than that of IgG1, causing the immune response of Th2 type bias.…”

Section: Discussionmentioning

confidence: 97%

Comparison of the immune response and protection against the experimental Toxoplasma gondii infection elicited by immunization with the recombinant proteins BAG1, ROP8, and BAG1‐ROP8

Xing,

Yang,

Yao

et al. 2024

Parasite Immunology

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Toxoplasmosis is one of the most dangerous zoonotic diseases, causing serious economic losses worldwide due to abortion and reproductive problems. Vaccination is the best way to prevent disease; thus, it is imperative to develop a candidate vaccine for toxoplasmosis. BAG1 and ROP8 have the potential to become vaccine candidates. In this study, rTgBAG1, rTgROP8, and rTgBAG1‐rTgROP8 were used to evaluate the immune effect of vaccines in each group by detecting the humoral and cellular immune response levels of BABL/c mice after immunization and the ability to resist acute and chronic infection with Toxoplasma gondii (T. gondii). We divided the mice into vaccine groups with different proteins, and the mice were immunized on days 0, 14, and 28. The protective effects of different proteins against T. gondii were analysed by measuring the cytokines, serum antibodies, splenocyte proliferation assay results, survival time, and number and diameter of brain cysts of mice after infection. The vaccine groups exhibited substantially higher IgG, IgG1, and IgG2a levels and effectively stimulated lymphocyte proliferation. The levels of IFN‐γ and IL‐2 in the vaccine group were significantly increased. The survival time of the mice in each vaccine group was prolonged and the diameter of the cysts in the vaccine group was smaller; rTgBAG1‐rTgROP8 had a better protection. Our study showed that the rTgBAG1, rTgROP8, and rTgBAG1‐rTgROP8 recombinant protein vaccines are partial but effective approaches against acute or chronic T. gondii infection. They are potential candidates for a toxoplasmosis vaccine.

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“…Largemouth bass injected with LMBV DNA vaccine encoding major capsid protein (MCP) also showed an up‐regulation of IL‐8 (Jia et al, 2020). Zhu et al (2021) found that IL‐12p40 expression was remarkably increased after DNA vaccine immunization in mice. These immune‐related gene upregulations further indicate that pcDNA3.1‐G plasmids induce a strong immune response after immunization of largemouth bass.…”

Section: Discussionmentioning

confidence: 99%

“…Similar findings have been found in other studies:Zhao et al (2019) found that IL-8 was upregulated in subunit vaccine. Largemouth bass injected with LMBV DNA vaccine encoding major capsid protein (MCP) also showed an up-regulation of IL-8(Jia et al, 2020) Zhu et al (2021). found that IL-12p40 expression was remarkably increased after DNA vaccine immunization in mice.…”

mentioning

confidence: 97%

Protective immunity by DNA vaccine against Micropterus salmoides rhabdovirus

Yang

Guo

Zhao

et al. 2022

Journal of Fish Diseases

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Micropterus salmoides rhabdovirus (MSRV) is one of the common pathogens in the largemouth bass industry, which can cause lethal diseases in juvenile fish and enormous economic losses. To establish effective means to prevent MSRV infection, the pcDNA3.1‐G plasmid containing the MSRV glycoprotein gene was successfully constructed and intramuscularly injected into the largemouth bass to evaluate the immune responses and protective effects in our study. As the results showed, the serum antibody levels of the fish vaccinated with different doses of pcDNA3.1‐G were significantly higher compared with the control groups (PBS and pcDNA3.1). Meanwhile, the immune parameters (acid phosphatase and alkaline phosphatase) were also significantly up‐regulated. Several immune‐related genes (IgM, IL‐8, IL‐12p40 and CD40) were expressed in the pcDNA3.1‐G groups at higher levels than in the control groups, which indicated that strong immune responses were induced. Besides, the survival percentages of fish in the control groups (PBS and pcDNA3.1) and pcDNA3.1‐G groups (2.5, 5, 10 and 20 μg/fish) at 14 days after challenge experiment with MSRV were 0%, 0%, 6.1%, 15.2%, 29.0% and 48.5% respectively. This study indicated that pcDNA3.1‐G was a prospective DNA vaccine candidate against MSRV‐induced mortality.

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Protective Immunity Induced by TgMIC5 and TgMIC16 DNA Vaccines Against Toxoplasmosis

Cited by 11 publications

References 39 publications

A novel Toxoplasma gondii TGGT1_316290 mRNA-LNP vaccine elicits protective immune response against toxoplasmosis in mice

A novel Toxoplasma gondii TGGT1_316290 mRNA-LNP vaccine elicits protective immune response against toxoplasmosis in mice

Comparison of the immune response and protection against the experimental Toxoplasma gondii infection elicited by immunization with the recombinant proteins BAG1, ROP8, and BAG1‐ROP8

Protective immunity by DNA vaccine against Micropterus salmoides rhabdovirus

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