Protective immunity induced by vaccination with two <i>Haemonchus contortus</i> excretory secretory proteins in sheep

Schallig, Henk D. F. H.; Leeuwen, M. A. W. Van; Cornelissen, A. W. C. A.

doi:10.1046/j.1365-3024.1997.d01-148.x

Cited by 93 publications

(87 citation statements)

References 30 publications

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“…The levels of immunoprotection observed in this study approached those obtained in goats using gut protein from H. contortus [10] and those achieved in sheep using excretory-secretory products [23], antigens purified from larvae [9] or different adult membrane antigens [7,17,26]. The percentages of egg and worm reduction were also comparable to those attained by Knox et al [14] in sheep using enriched cysteine proteases purified from Triton X-100 soluble membrane-bound protein extracts from adult worms (S3).…”

Section: Discussionsupporting

confidence: 77%

“…The immunoprophylactic properties of excretory-secretory products [23], different adult membrane antigens, such as H11 [17], H-gal-GP [26] or p26/23 [7] and antigens purified from larvae, such as the Hc-SL3 antigen [9] have been investigated. Cysteine proteases, because of their apparent critical function in worm nutrition, immune evasion or both [21] have also been assessed as vaccine candidates.…”

Section: Introductionmentioning

confidence: 99%

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Immunoprotection in goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions

et al. 2004

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-Haemonchus cysteine proteases, because of their apparent critical function in worm physiology, are considered important candidates in the immunological control of haemonchosis in sheep. Only limited information is, however, available on the immunoprotective properties of these molecules in goats. In the present study cysteine proteases of Haemonchus contortus adult worms isolated from a goat strain (Gran Canaria, Spain) were enriched by affinity chromatography and evaluated as immunoprotective antigens against caprine haemonchosis. The eggs per gram of faeces averaged over the whole experiment for unvaccinated goats (550 ± 13.5) was significantly greater (P < 0.001) than that of vaccinated goats (61 ± 2.9). Accordingly, the worm burden was significantly different between the groups (P < 0.05), with mean values of 247.5 ± 43.8 and 762.5 ± 78.3 worms per animal in the immunized and nonimmunized goats, respectively. The percentage of egg (89%) and worm (68%) reduction approached those attained with other immunogens used in sheep. Haemonchus contortus / cysteine proteases / immunoprotection / goats

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Immunoprotection in goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions

et al. 2004

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“…Obtained after in vitro cultivation of adult worms in serum-free medium, ESP or its partially purified fractions are able to induce 65 to 90% protection against H. contortus in sheep (1,2), but protective properties have not conclusively been attributed to individual proteins.…”

Section: Gastrointestinal (Gi)mentioning

confidence: 98%

Comprehensive Analysis of the Secreted Proteins of the Parasite Haemonchus contortus Reveals Extensive Sequence Variation and Differential Immune Recognition

Yatsuda

Krijgsveld

Cornelissen

et al. 2003

Journal of Biological Chemistry

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194

164

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Haemonchus contortus is a nematode that infects small ruminants. It releases a variety of molecules, designated excretory/secretory products (ESP), into the host. Although the composition of ESP is largely unknown, it is a source of potential vaccine components because ESP are able to induce up to 90% protection in sheep. We used proteomic tools to analyze ESP proteins and determined the recognition of these individual proteins by hyperimmune sera. Following two-dimensional electrophoresis of ESP, matrix-assisted laser desorption ionization time-of-flight and liquid chromatographytandem mass spectrometry were used for protein identification. Few sequences of H. contortus have been determined. Therefore, the data base of expressed sequence tags (dbEST) and a data base consisting of contigs from Haemonchus ESTs were also consulted for identification. Approximately 200 individual spots were observed in the two-dimensional gel. Comprehensive proteomics analysis, combined with bioinformatic search tools, identified 107 proteins in 102 spots. The data include known as well as novel proteins such as serine, metallo-and aspartyl proteases, in addition to H. contortus ESP components like Hc24, Hc40, Hc15, and apical gut GA1 proteins. Novel proteins were identified from matches with H. contortus ESTs displaying high similarity with proteins like cyclophilins, nucleoside diphosphate kinase, OV39 antigen, and undescribed homologues of Caenorhabditis elegans. Of special note is the finding of microsomal peptidase H11, a vaccine candidate previously regarded as a "hidden antigen" because it was not found in ESP. Extensive sequence variation is present in the abundant Hc15 proteins. The Hc15 isoforms are differentially recognized by hyperimmune sera, pointing to a possible specific role of Hc15 in the infectious process and/or in immune evasion. This concept and the identification of multiple novel immune-recognized components in ESP should assist future vaccine development strategies.

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“…Several trials of vaccination with ESP were carried out against common sheep parasites such as Fasciola hepatica [30,34] and Haemonchus contortus [27] or myiasis producing species such as Lucilia cuprina in sheep [28,29,32,33] or Hypoderma lineatum in cattle [2,9,24,26] resulting in the reduced parasite burdens and/or fecundity or larval growth.…”

Section: Introductionmentioning

confidence: 99%

Immunisation of lambs with excretory secretory products of Oestrus ovis third instar larvae and subsequent experimental challenge

Frugère¹,

Leon

Prévot³

et al. 2000

Vet. Res.

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-Excretory-secretory products (ESP) of myiasis producing agents are involved in nutrition and development of larvae and are often immunogens. This study was carried out in order to define the antigenicity, the immunogenicity of Oestrus ovis ESP and the role of sheep immune response to ESP. Twenty-four six to eight month old female lambs were randomly allocated into two groups. The first one was immunised twice, four weeks apart, with excretory-secretory products of Oestrus ovis third instar larvae (L3ESP) in complete then incomplete Freund adjuvant. The second one served as a control, and received two injections of PBS plus complete and incomplete Freund adjuvant. Fifteen and twenty-eight days after the second immunisation, animals of both groups were experimentally challenged with O. ovis first instar larvae. Twelve days after the second experimental challenge, the twenty-four lambs were necropsied. The total number of O. ovis larvae, their stages of development, weights and sizes were recorded per animal and compared between the two groups. Establishment rates were very similar in both groups: 39% and 35% in control and vaccinated groups respectively but the percentage of developing stages was higher in the control group (13%) than in the vaccinated group (6%). It was concluded that the L3ESP immunisation of sheep did not protect against larval establishment but provided an inhibitory effect on larval growth.Oestrus ovis / excretory-secretory products / sheep / immunisation / experimental challenge Vet. Res. 31 (2000) 527-535 527

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Protective immunity induced by vaccination with two Haemonchus contortus excretory secretory proteins in sheep

Cited by 93 publications

References 30 publications

Immunoprotection in goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions

Immunoprotection in goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions

Comprehensive Analysis of the Secreted Proteins of the Parasite Haemonchus contortus Reveals Extensive Sequence Variation and Differential Immune Recognition

Immunisation of lambs with excretory secretory products of Oestrus ovis third instar larvae and subsequent experimental challenge

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