Protective immunity induced in mice by F0 and FII antigens purified from Paracoccidioides brasiliensis

Vaccination with Heat Shock Protein 60 Induces a Protective Immune Response against ExperimentalParacoccidioides brasiliensisPulmonary Infection

“…The immunization of mice with F0 and FII, predominantly surface-localized antigens, elicited a protective effect against highly infective yeast forms of P. brasiliensis. Although the FIII fraction induces IFN-␥, this property did not result in protection against the challenge infection (21,23). Previously, we had identified H. capsulatum HSP60 as a prominent target of T-cell-mediated immunity and a protective vaccine in experimental pulmonary histoplasmosis (18,44).…”

“…We utilized a model of respiratory P. brasiliensis infection in BALB/c mice. This strain has been used previously by several groups for analysis of pulmonary infection with P. brasiliensis (3,16,21,25,33,41). Mice inoculated with 1 ϫ 10 6 or 1 ϫ 10 7 P. brasiliensis yeasts developed a predictable course of infection, in which the infectious agent could be recovered and quantified up to 12 weeks after the initial challenge.…”

Interleukin-10 and Tumor Necrosis Factor–α Single Nucleotide Gene Polymorphism Frequency in Paracoccidioidomycosis

“…Our study was designed to evaluate the potential association of cytokine gene polymorphism and paracoccidioidomycosis. Among the cytokines characterized in PCM, we studied TNF-␣ and IL-10, as both cytokines were found at high levels in patients and in experimental PCM [4,15,24,25,26]. TNF-␣ is a cytokine that may play a significant role in the genesis and in the control of some aspects of the disease, including granulomatous reaction, hypergammaglobulinemia, and depression of T-cell-mediated immunity [16,26,27].…”

“…Although IL-10 limits inflammatory responses, tumor necrosis factor-␣ (TNF-␣) determines the strength and the length of local and systemic inflammatory reactions [11]. This cytokine is involved in the resistance to Pb infection, granuloma formation, and control of fungus dissemination [4,15]. In addition, TNF-␣ modulates the production of other cytokines and nitric oxide, as well as the T-cell proliferative response in Pb-infected mice [15,16].…”

“…This cytokine is involved in the resistance to Pb infection, granuloma formation, and control of fungus dissemination [4,15]. In addition, TNF-␣ modulates the production of other cytokines and nitric oxide, as well as the T-cell proliferative response in Pb-infected mice [15,16]. The gene of TNF-␣ is located within the MHC region in vertebrate species [17].…”