Protective Role of Catechin on d-Galactosamine Induced Hepatotoxicity Through a p53 Dependent Pathway

Abstract

Objective of this study was to obtain a better understanding of the mechanism responsible for the d-galactosamine (d-GalN) induced hepatotoxicity and to study the effect of catechin against d-GalN induced hepatotoxicity. Catechin 50 and 100 mg/kg b.wt was administered for 1 week by oral route. Liver damage was induced by intra-peritoneal administration of 400 mg/kg b.wt d-galactosamine on the last day of catechin treatment. At the end of treatment all animals were killed and liver enzyme levels were estimated. Dissected hepatic samples were used for histopathology, RNA isolation, expression studies of Bax, Bcl-2 and p53 mRNA levels and mitochondrial membrane potential studies. We found that increases in the liver enzyme activity and decrease in antioxidant enzyme activity by d-GalN were significantly restricted by oral pretreatment with catechin. Disruption of mitochondrial membrane potential, up regulation of p53, Bax and down regulation of Bcl-2 mRNA levels in the liver of d-GalN intoxicated rats were effectively prevented by pretreatment with catechin.

Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCl4-induced liver injury in rodents

“…In the present work, the effect of TPW on liver mitochondrial membrane potential in CCl 4 intoxicated rat was assessed. Treatment of rats with CCl 4 , damaged the liver mitochondria as characterized by the dissipation of the mitochondrial membrane potential, which is in agreement with previously published reports [13]. TPW could maintain the integrity of the mitochondrial membrane, which confirmed its protective effect through an antioxidant mechanism.…”

“…After freezing, homogenates (5% w/v) were prepared and centrifuged at 1000 rpm for 10 min using a refrigerated centrifuge (MIKRO 22R, Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany). The supernatant was used for the estimation of glutathione (GSH), malondialdehyde (MDA) hydroperoxides, superoxide dismutase (SOD) and catalase levels [13]. …”

“…Mitochondria were isolated from rat liver as previously described [13]. In brief, the tissue was manually homogenized by four strokes with a Teflon pestle in solution I [230 mM mannitol, 70 mM sucrose, 1 mM EGTA and 5 mM HEPES (pH 7.4)] on ice.…”

Modulatory influence of Acacia hydaspica R. Parker ethyl acetate extract against cisplatin inveigled hepatic injury and dyslipidemia in rats

“…Catechin is the major compounds of AHE, and previous studies affirm the hepatoprotective effect of catechin against chemically induced hepatotoxicity. The mechanism of protective effect by catechin was inhibition of oxidative stress, increase antioxidant status and inhibition of apoptosis in hepatocytes [ 63 ]. Hence the presence of polyphenols in AHE might be responsible for the hepatoprotective effect by reducing oxidative stress.…”

Assessment of preclinical effect of (+)‐catechin hydrate on sexual function: An in silico and in vivo study

“…Catechins are the major group of compounds present in green tea that possess a lot of beneficial effects such as antioxidants (Rai et al, 2019), anti-inflammatory (Trekli, Buttle, & Guesdon, 2004), neuroprotection (Cheruku et al, 2018(Cheruku et al, , 2019, antidepressant (Rai et al, 2019), anticancer (Jain et al, 2013), cardioprotection (Babu & Liu, 2008), hepatoprotection (Vasanth Raj et al, 2010) and many more (Saimithra, Kumar, Nandakumar, Nampoothiri, & Rao, 2018;Singh, Shankar, & Srivastava, 2011). In addition to that, catechins are reported to alter the neurotransmitter levels especially monoamines.…”