Protective Role of Selenium Compounds on the Proliferation, Apoptosis, and Angiogenesis of a Canine Breast Cancer Cell Line

Abstract

We herein examined the effects of different doses, forms, and compatibilities of selenium on a canine mammary gland tumor cell line, CTM1211, and explored the related mechanisms. Three selenium compounds, sodium selenite (SSE), methylseleninic acid (MSA), and methylselenocysteine (MSC), were selected for these experiments, and cyclophosphamide (CTX) served as a positive control. In the cell viability assay, the cell viability of each group at 48/72 h decreased significantly compared with the control group (p < 0.05), and the cell viability of the CTX + MSA group was lower than that of CTX and MSA groups (p < 0.05). Moreover, the inhibitory effect of selenium on cell proliferation was time-dependent but not concentration-dependent. In the cell apoptosis assay, the apoptosis values of each group increased significantly compared with the control group, and the apoptosis values of the CTX + MSA group increased the most significantly (p < 0.01). The protein and mRNA expression levels of vascular endothelial growth factor-alpha (VEGF-alpha), angiopoietin-2 (Ang-2), and hypoxia inducible factor-1 alpha (HIF-1 alpha) were downregulated in each group, while that of phosphatase and tensin homolog (PTEN) were upregulated (p < 0.05). In conclusion, these three selenium compounds, especially MSA, could significantly inhibit the viability and growth of the CTM1211 cell line, which is partly due to the induction of apoptosis and regulation of tumor angiogenesis.

Protective Effect of Se-Methylselenocysteine on Elaidic Acid-Induced Inflammation in Human Arterial Endothelial Cells

“…The most appropriate concentration and duration of MSC to incubate with HAECs might be 200 mmol/L and 24 h. The apoptosis rate of HAECs increased obviously when co-incubated with EA1 400 mmol/L MSC may due to cytotoxicity of MSC at high concentration (17). Reported studies approved that the most two common existence forms of selenium, inorganic selenium (such as sodium selenite) and organic selenium (such as methylseleninic acid and Se-methylselenocysteine) may exert bioactive or cytotoxic effects in vitro strictly depend on compound, concentration and model used (14,18). We use MSC in our model because increasing studies supported that MSC has stronger bioactivity than other forms of selenium in vitro (19).…”

“…Briefly, cells were divided into 5 groups: negative control (NC) group, elaidic acid (EA) group (50 mmol/L), EA1 MSC group (50 mmol/L1 100 mmol/L, 50 mmol/L1 200 mmol/L, 50 mmol/L1 400 mmol/L). The concentrations of EA and MSC used in this study were according to previous study (14,15). Three thousand cells per well were seeded into 96-well plates and incubated for 24 h and 48 h with different reagents.…”

Isoselenocyanates: Synthesis and Their Use for Preparing Selenium-Based Heterocycles

“…[15][16][17][18] Despite the complex and ambiguous mechanisms of action, organoselenium compounds are proposed to induce DNA damage, regulate the cell cycle, inhibit cellular growth, induce apoptosis and generate ROS. [15][16][17][18][19] Furthermore, it has been reported that patients with melanoma, breast cancer, and pancreatic cancer have exhibited decreased selenium levels in whole blood or serum compared to healthy controls. 20 In spite of the mentioned importance of selenium and organoselenium compounds, there is evidence showing contradictory antioxidant behaviors in selenium containing compounds, suggesting that these compounds must be examined individually for its antioxidant conduct.…”

Novel seleno-hydantoin palladium(II) complex – antimigratory, cytotoxic and prooxidative potential on human colon HCT-116 and breast MDA-MB-231 cancer cells

“…On the other hand, selenium has proven to be an important supplement for the suppression of the side effects of chemotherapy (Schroeder et al 2004;Markovic et al 2011;Brodin et al 2015;Mut-Salud et al 2016). Also, selenium-containing compounds exert inhibition of migration of cancer cells (Zec et al 2012;Chen et al 2013;Liu et al 2015). Combining palladium and selenium in the same structure could provide a novel substance with benefits from the both elements.…”