Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugarbeet calluses

Hagège, Daniel; Kevers, Claire; Salabert, Philippe; Gaspar, Thomas

doi:10.1007/bf02823063

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…Catalase (EC 1.11.1.6) activity was assayed by consumption of H 2 O 2 according to Hagège et al (1992). The total activity of superoxide dismutase (SOD) (EC 1.15.1.1) was determined from its capacity to inhibit reduction of tetrazolium blue by superoxide radicals (O 2…”

Section: Determination Of Protective Enzymes Activitiesmentioning

confidence: 99%

“…Recently, we have demonstrated a relationship between phenotypes of these cell lines, their ligniWcation during cell diVerentiation and their DNA methylation levels (Causevic et al 2005). Previous reports on cellular redox state parameters have been published in other independent works only for the DD and NO lines and have revealed an oxidative stress state in the DD line (Hagège et al 1992;Cerrie et al 1994;Cherif et al 1996;Hagège 1996). These cell lines represent an interesting model for the study of plant morphogenesis; and reactive oxygen species (ROS) are believed to play an important role as intracellular messengers during morphogenesis (Sauer et al 2001;Boonstra and Post 2004;Mittler et al 2004;Edreva 2005).…”

Section: Introductionmentioning

confidence: 97%

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Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

Causevic

Gentil

Delaunay

et al. 2006

Planta

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In order to evaluate the permanent chromatin remodeling in plant allowing their high developmental plasticity, three sugarbeet cell lines (Beta vulgaris L. altissima) originating from the same mother plant and exhibiting graduate states of differentiation were analyzed. Cell differentiation has been estimated by the cell redox state characterized by 36 biochemical parameters as reactive oxygen species steady-state levels, peroxidation product contents and enzymatic or non-enzymatic protective systems. Chromatin remodeling has been estimated by the measurement of levels of DNA methylation, histone acetylation and corresponding enzyme activities that were shown to differ between cell lines. Furthermore, distinct loci related to proteins involved in cell cycle, gene expression regulation and cell redox state were shown by restriction landmark genome scanning or bisulfite sequencing to display differential methylation states in relation to the morphogenic capacity of the lines. DNA methylating, demethylating and/or histone acetylating treatments allowed to generate a collection of sugarbeet cell lines differing by their phenotypes (from organogenic to dedifferentiated), methylcytosine percentages (from 15.0 to 43.5%) and acetylated histone ratios (from 0.37 to 0.52). Correlations between methylcytosine or acetylated histone contents and levels of various parameters (23 or 7, respectively, out of 36) of the cell redox state could be established. These data lead to the identification of biomarkers of sugarbeet morphogenesis in vitro under epigenetic regulation and provide evidence for a connection between plant morphogenesis in vitro, cell redox state and epigenetic mechanisms.

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Section: Determination Of Protective Enzymes Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

Causevic

Gentil

Delaunay

et al. 2006

Planta

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“…-produced by the xanthine/xanthine oxidase system, was measured by the decline in absorbance at 560 nm for 8 min (adapted from [7]). One SOD unit was taken as the amount of extract that gave 50% inhibition of reduction of tetrazolium blue.…”

Section: Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Impact of drought and leaf development stage on enzymatic antioxidant system of twoPopulus deltoides×nigraclones

Marron

Maury²,

Rinaldi³

et al. 2006

Ann. For. Sci.

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-Impacts of mild and severe water constraints were investigated on leaf protein content and activities of superoxide dismutase (SOD), catalase and peroxidase in young cuttings of two Populus deltoides × nigra clones, 'Luisa_Avanzo' and 'Dorskamp', known to differ in their level of drought tolerance. Expanding and mature leaves were analyzed separately. The effect of water deficit on enzymatic antioxidant system depended on both clone and leaf development stage. For 'Dorskamp', which presents an higher drought tolerance than 'Luisa_Avanzo', activities of SOD and catalase increased in response to the severe water deficit in mature leaves only. For 'Luisa_Avanzo', peroxidase activity increased in response to the mild water deficit in expanding leaves merely. For both clones, three different SOD isoforms, Mn-SOD, Fe-SOD and Cu/Zn-SOD were detected in various amounts depending on drought intensity.water deficit / leaf development stage / catalase / superoxide dismutase / peroxidase Résumé -Impact de la sécheresse et du stade de développement des feuilles sur les systèmes antioxydants enzymatiques de deux clones de Populus deltoides × nigra. L'impact de sécheresses modérée et sévère sur le contenu en protéines des feuilles et sur les activités de la dismutase de superoxyde (SOD), de la catalase et de la peroxydase a été étudié chez de jeunes boutures de deux clones de Populus deltoides × nigra, 'Luisa_Avanzo' et 'Dorskamp', connus pour présenter des niveaux différents de tolérance au déficit hydrique. Les feuilles en croissance et matures ont été analysées séparément. La réponse à la sécheresse des systèmes de protection enzymatiques était différente selon le clone et le stade de développement des feuilles étudiées. Pour le clone 'Dorskamp', réputé plus tolérant que 'Luisa_Avanzo' à la sécheresse, les activités de la SOD et de la catalase augmentaient dans les feuilles matures en réponse à un déficit hydrique sévère. Pour le clone 'Luisa_Avanzo', l'activité de la peroxydase augmentait essentiellement dans les feuilles en croissance en réponse à une sécheresse modérée. Pour les deux clones, trois isoformes différentes de la SOD, la Mn-SOD, la Fe-SOD et la Cu/Zn-SOD ont été détectées en quantités variables chez les deux clones en fonction de l'intensité de la contrainte hydrique. déficit hydrique / stade de développement foliaire / catalase / dismutase de superoxyde / peroxydase

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“…The H callus which lacks chlorophylls is deficient in other tetrapyrrole-containing compounds (Hag6ge et al 1992c). The low levels of catalase (Hag6ge et al 1992b) and peroxidases (Kevers et al 1981) indicate that H20 2 could not be detoxified. The lack of peroxidase activities is evidenced by the fact that the lignin polymerisation does not occur in the H callus.…”

Section: Habituation and The Protective Systems Against Activated Oxymentioning

confidence: 99%

“…The deficiency in tetrapyrrole synthesis has recently been explained by the lack of enzymes of the Beale pathway (Hag6ge et al 1992c), which has been confirmed by the reduction of 5-aminolevulinate synthesis (results not shown). The high levels of SOD and GR (Hag6ge et al 1992b) are indicative of an oxygen stress (Hassan & Scandalios 1990;Smith et al 1989). In these conditions, H20 2 accumulation leads to generation, through the Fenton reaction (Elstner 1982), of a high level of hydroxyl radicals (OH') which might contribute to lipid peroxidation.…”

Section: Habituation and The Protective Systems Against Activated Oxymentioning

confidence: 99%

Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)?

Dily

Huault

Gaspar

et al. 1993

Plant Cell Tiss Organ Cult

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The habituated callus is a vitrified tissue which has two main biochemical characteristics both leading to production of toxic forms of oxygen: first the blockage of the porphyrin pathway and a lack of H20 2 detoxifying enzymes (catalase and peroxidases); secondly a deviation of the nitrogen metabolism induced by NH 3 accumulation. Ammonia detoxification is ensured by increased glutamate dehydrogenase activity and accumulation of both proline and polyamines. A putative linkage between proline synthesis and the HMP pathway, as proposed for animal proliferating tissues (Phang 1985), might explain a high purine biosynthesis and cytokinin autonomy.Abbreviations: FFA-free fatty acids, 6PG-DH-6-phosphogluconate dehydrogenase, G6P-DH-

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Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugarbeet calluses

Cited by 14 publications

References 26 publications

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

Impact of drought and leaf development stage on enzymatic antioxidant system of twoPopulus deltoides×nigraclones

Does altered nitrogen metabolism and H2O2 accumulation explain the vitrified status of the fully habituated callus of Beta vulgaris (L.)?

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