Protein adducts: quantitative and qualitative aspects of their formation, analysis and applications

Törnqvist, Margareta; Fred, Charlotta; Haglund, Johanna; Helleberg, Hans; Paulsson, Birgit; Rydberg, Per

doi:10.1016/s1570-0232(02)00172-1

Cited by 299 publications

(377 citation statements)

References 165 publications

Supporting

Mentioning

370

Contrasting

Unclassified

Order By: Relevance

“…Monitoring of hemoglobin (Hb) adducts is an established methodology for measuring the internal dose after exposure to reactive chemicals (Törnqvist et al, 2002). This methodology has been applied in studies of AA exposure in for example, occupational environments (reviewed by Törnqvist et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

et al. 2007

View full text Add to dashboard Cite

Objective: To examine the coherence of estimated intakes of acrylamide (AA) from foods, with hemoglobin (Hb) AA adduct levels, an objective marker of environmental AA exposure. Design: A cross-sectional study. Setting: The Malmö Diet and Cancer study, a large population-based prospective cohort (n ¼ 28 098) in the south of Sweden. Subjects: A sample of non-smoking (n ¼ 70) and smoking (n ¼ 72) women and men selected to obtain large variation in Hb AA adducts. Methods: Self-reported data on the usual consumption of foods were combined with published data on the AA content in Swedish foods. The Hb AA adduct levels were determined by a modified Edman degradation method. Linear regression and correlation analysis examined associations between estimated AA intakes, and Hb AA adducts. Results: In randomly selected individuals (n ¼ 40), the estimated median AA intake was 28 mg per day. In linear regression models, adjusting for sex, significant associations were seen in non-smokers between Hb AA adducts and estimated AA from foods (P ¼ 0.006). In smokers both AA from foods (P ¼ 0.006) and the calculated amount of tobacco consumed (P ¼ 0.003) were significantly associated with Hb AA adducts. Positive partial correlations between dietary AA estimates and Hb AA adducts were seen in smoking men (r ¼ 0.37) and women (r ¼ 0.59), and in non-smoking men (r ¼ 0.60), but not in non-smoking women. Conclusions: This study suggests that both diet and tobacco are important sources of the environmental AA exposure, although the lack of correlations in non-smoking women cast doubt on the validity of dietary AA intake estimates used in cancer epidemiology, or suggest that unrecognized factors may influence the internal dose measure of AA exposure.

show abstract

Section: Introductionmentioning

confidence: 99%

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Furthermore, the possibility of establishing absolute quantitative relationships between levels of biomarker (internal dose) and intake considerably strengthens the usefulness of such analytical techniques. In this context measurement of adducts to N-terminal valines in Hb by the method used, applicable in laboratory animals and humans, has many advantages [51]. Measurement of the adduct from AA to cysteine in Hb, which also has been applied in animal studies [52,53], is less useful (reviewed in [51]).…”

mentioning

confidence: 99%

“…In this context measurement of adducts to N-terminal valines in Hb by the method used, applicable in laboratory animals and humans, has many advantages [51]. Measurement of the adduct from AA to cysteine in Hb, which also has been applied in animal studies [52,53], is less useful (reviewed in [51]). Furthermore, studies are rendered difficult if the adduct is formed as an artefact which has been shown to occur in the case of ethylene oxide, but not for AA, during storage of blood samples.…”

mentioning

confidence: 99%

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Hagmar

Wirfält

Paulsson

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

“…Protein adducts have been used as long-term exposure biomarkers for many compounds, mainly carcinogens (Törnqvist et al 2002). Since the levels of protein adducts are related to the DNA adducts it has been suggested that the protein adducts better show the risk of the exposure to carcinogens than monitoring of metabolites only.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of exposure in Monday morning urine samples as a long-term measure of exposure to aromatic diisocyanates

Tinnerberg

Broberg

Lindh

et al. 2013

Int Arch Occup Environ Health

View full text Add to dashboard Cite

Protein adducts: quantitative and qualitative aspects of their formation, analysis and applications

Cited by 299 publications

References 165 publications

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

Associations between estimated acrylamide intakes, and hemoglobin AA adducts in a sample from the Malmö Diet and Cancer cohort

Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender

Biomarkers of exposure in Monday morning urine samples as a long-term measure of exposure to aromatic diisocyanates

Contact Info

Product

Resources

About