Protein aggregation in foam fractionation of bovine serum albumin: Effect of protein concentration

Li, Rui; Fu, Ning; Wu, Zhaoliang; Wang, Yanji; Wang, Yanyan

doi:10.1016/j.bej.2015.08.006

Cited by 15 publications

(15 citation statements)

References 33 publications

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“…In the experiments, the BSA sample without aggregates was prepared by size exclusion chromatography according to the work of [17]. It was used to prepare the BSA solutions of 0.30 g/L with pH 7.0, 6.0, 5.0, 4.7, 4.0 and 3.0.…”

Section: Resultsmentioning

confidence: 99%

“…The measurements of Γ and r 32 had been specifically described in the previous work of Li et al [17] with the pictures for measuring r 32 taken at the top of the foam fractionation column. The results are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The former was defined as Eq. (1) while the latter and all the BSA concentrations were determined as the previous work of Li et al [17] with a flow rate of mobile phase of 0.7 mL/min.

E = \frac{C_{f}}{C_{o}}

where C f and C o are the BSA concentrations in the foamate and the feed BSA solutions, respectively, g/L.…”

Section: Methodsmentioning

confidence: 99%

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Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin

Wangb

et al. 2016

Biotechnology Reports

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177

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin

Wangb

et al. 2016

Biotechnology Reports

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177

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“…The protein concentration in the aqueous extract of Semen Cassiae increased with C 0 . Increasing protein concentration could decrease the protein enrichment ratio and increase the protein recovery yield [30]. Furthermore, the anthraquinones in the extract had good protein-binding affinity.…”

Section: Effects Of Vsts On E and Rmentioning

confidence: 99%

Separation of Protein-Binding Anthraquinones from Semen Cassiae Using Two-Stage Foam Fractionation

Ding

Wang²,

Yue

et al. 2019

Processes

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Anthraquinones are compounds of high medicinal value in many plants. Based on their good protein binding affinity, foam fractionation was attempted to separate them using proteins in the aqueous extract of Semen Cassiae as collectors. Firstly, the interaction between anthraquinones and Semen Cassiae proteins has been analyzed by the Stem–Volmer equation with physcion as a standard. The results show that physcion had good interaction with the proteins via hydrophobic forces. More importantly, the proteins effectively assisted the foam fractionation of several anthraquinones including aurantio-obtusifolin, aloe-emodin, rhein, emodin, chrysophanol, and physcion. On this basis, a two-stage foam fractionation technology was developed for process intensification using a foam fractionation with vertical sieve trays (VSTs). VSTs, initial feed concentration of total anthraquinones, temperature, volumetric air flow rate and pore diameter of gas distributor had significant effects on enrichment ratio and recovery yield of anthraquinones. Under suitable conditions, the enrichment ratio of total anthraquinones reached 47.0 ± 4.5 with a concentration of 939 ± 94 mg/L in the foamate while their total recovery percentage reached more than 47.7%. In addition, foam fractionation also increased the purity and hydroxyl radical scavenging activity of total anthraquinones. The results had significant implications for the separation of anthraquinones from Semen Cassiae.

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“…In foam fractionation of proteins, a certain loss activity is often observed due to structural unfolding of protein molecules induced by the gas–liquid interface . In addition, the lower protein concentration results in a larger activity loss . Li et al used beta‐cyclodextrin to prevent the interface‐induced BSA aggregation and bromelain denaturation effectively.…”

Section: Introductionmentioning

confidence: 99%

Foam fractionation for enhancing silica gel adsorption of urokinase from human urine

Wang

Dou

et al. 2019

Asia-Pacific J Chem Eng

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In this work, foam fractionation (FF), a cost-effective method, was used to enhance the silica gel adsorption (SGA) of urokinase from human urine. The analysis of how FF enhanced the adsorption/desorption of urokinase onto silica gel was conducted. The results show that FF increased both the adsorption rate of urokinase onto silica gel and the activity concentration of urokinase adsorbed at silica gel by pre-enriching urokinase from urine. It also reduced the activity loss of urokinase in the desorption process. Then, a coupled technology of FF and SGA was developed. The effects of pH, superficial air flow rate, beta-cyclodextrin concentration, and amount of silica gel added on activity recovery yield and purification fold of urokinase were studied. At pH 7.5, superficial air flow rate 1.70 mm/s, beta-cyclodextrin concentration 0.2 g/L, and amount of silica gel added 0.9%, the activity recovery and purification fold of urokinase reached 89.5% and 56.8 in the coupled technology, which were 25.3% and 79.2% higher than those obtained by SGA alone, respectively. Furthermore, the amount of silica gel added in the coupled technology was 40.0% lower than that in SGA alone. Therefore, FF effectively enhanced the SGA of urokinase from human urine.

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Protein aggregation in foam fractionation of bovine serum albumin: Effect of protein concentration

Cited by 15 publications

References 33 publications

Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin

Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin

Separation of Protein-Binding Anthraquinones from Semen Cassiae Using Two-Stage Foam Fractionation

Foam fractionation for enhancing silica gel adsorption of urokinase from human urine

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