Protein Associated with Hyaluronic Acid in Ox Synovial Fluid

Abstract

SummaryProtein which is associated with hyaluronic acid, prepared in a physicochemically unchanged state from ox synovial fluid by ultrafiltration, has been separated by preparative equilibrium sedimentation in a caesium chloride density gradient and by chromatography on DEAE-Sephadex. Earlier work has been confirmed by showing that the protein consists of at least two chemically distinct lipoprotein, components. It has also been confirmed that its removal from the hyaluronic acid causes marked changes of viscosity, without the occurrence of degradation of the hyaluronic acid. The separated proteins appeared in a number of differently aggregated forms, and this fact has prevented our drawing conclusions about their molecular sizes in synovial fluid.

Alexander G. Ogston’s Centennial: Researcher of hyaluronic acid and other biopolymers

“…Ogston and two associates published a paper in 1969, in which, first of all, they reaffirmed that the hyaluronic acid preparation from ox synovial fluid using mild filtration techniques contained about 20 to 30% protein [32]. They referred to previous work by Ogston and his co-workers, including a study by Ogston and Sherman dealing with the electrophoretic removal of protein from the complexes of hyaluronic acid and protein [33].…”