Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Abstract

Idrees et al.: Protein baits, volatile compounds and irradiation influence the expression profiles of odorant-binding protein genes inBactrocera dorsalis Fuzhou, Fujian, 350002, *Corresponding author e-mail: jiqinge@yeah.net; phone: +86-0-59-183-789-420; fax:+86-0-59-183-789-421 (Received 4 th Jul 2017; accepted 18 th Oct 2017)Abstract. Chemical communication in insects is based on odorant-binding proteins (OBPs), but the molecular mechanisms by which these OBPs allow the perception of volatiles and host chemicals remain unclear. Bactrocera dorsalis (Hendel) is one of the most economically important pests of fruits and vegetables. In this study, the OBPs of B. dorsalis (BdorOBPs) were evaluated in response to different attractive protein baits, brewer's yeast volatile compounds and irradiation. The expression of 10 genes encoding OBPs in the antennae of B. dorsalis during three adult physiological life stages (pre-mating, post-mating and post-oviposition) were analysed. All selected BdorOBP genes were found to contain one conserved pheromone-binding protein/general-odorant-binding protein domain (PBP-GOBP), except OBP8, which contained two of these conserved domains. An unrooted phylogenetic tree was constructed to show the relationships among these 10 BdorOBPs and the OBPs belonging to the same OBP family in other insects. We found significantly different transcript expression profiles in each OBP gene at each stage in response to different treatments; these results revealed that OBP2 expression was significantly increased in response to baits at each adult physiological stage, while OBP2, OBP5 and OBP1 were highly expressed in response to combined-volatiles treatment at all tested physiological stages. OBP3, OBP5 and OBP10 showed high expression in response to irradiation at all tested physiological stages. Thus, we infer that protein baits, brewer's yeast volatiles and irradiation significantly influence the transcript levels of OBP genes, which may act in olfactory perception.

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“…Recent studies have demonstrated that adult dietary quality, exposure to volatile compounds, and copulation impact the expression profile of OBPs and CSPs in several fruit fly species. Oriental fruit fly, B. dorsalis, exposed to attractive protein baits and volatiles from brewer’s yeast showed significantly different transcript expression profiles, and showed high expression of several OBPs including OBP2, OBP5 and OBP1 33 . Female B. tryoni mated with zingerone-fed males had different OBP expression profiles at the post-mating stage, while OBP 56a and genes encoding cuticle proteins were down regulated 32 .…”