Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC

Abstract

This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA (v/v) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779–0.980. The fingerprint chromatogram of Ranae Oviductus showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified Ranae Oviductus from the samples. These results indicated that the method established in this work was reliable.

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

“…As a precious natural product in northeast China, Oviductus Ranae has been used for nearly thousands of years and has developed into a series of nutrition, health food and traditional Chinese medicine. Oviductus Ranae is rich in nutrients, including a variety of unsaturated fatty acids (UFAs), protein, vitamins and steroids [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Among them, UFAs, as important bioactive substances, have excellent biological activities in regulating immunity and preventing a series of cardiovascular and cerebrovascular diseases such as hyperlipidemia, hypertension and thrombosis [ 10 , 11 , 12 , 13 , 14 ].…”

Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases

“…Oviductus Ranae is the dried oviduct of female Rana temporaria chensinensis David, which is mainly produced in Changbai Mountain area, Jilin Province, China [ 17 , 18 , 19 ]. As a nutritional and health supplement for both medicine and food, Oviductus Ranae is rich in protein, sterol, unsaturated fatty acid and other nutrients [ 20 , 21 , 22 , 23 , 24 , 25 ].…”

Rana chensinensis Ovum Oil Based on CO2 Supercritical Fluid Extraction: Response Surface Methodology Optimization and Unsaturated Fatty Acid Ingredient Analysis

“…In the Rana chensinensis industry, Rana chensinensis ovum (RCO) is the residual by-product in the production process of Oviductus Ranae ( Figure 1 ) [ 1 , 2 , 3 , 4 , 5 , 6 ]. Due to the relatively small amount of research on the by-products, RCO is usually treated as waste, causing serious wastage of Rana chensinensis resources.…”