Protein Biosynthesis and Oxidative Phosphorylation in Isolated Rat Liver Mitochondria

The Biogenesis of Mitochondria

“…The immediate stimulus to a serious search for mitochondrial DNA de rived from three separate lines of investigation; (a) yeast cells exposed to acriflavine give rise to mutants, the mutation is inherited in non-Mendelian fashion, and all such mutants lack cytochrome oxidase, a typical mitochon drial enzyme complex (1,2); (b) DNA had already been detected in chlo roplasts (3,4) and in trypanosomal kinetoplasts (5 ); both cytoplasmic or ganelles similar to mitochondria; and (c) isolated mitochondria are known to be capable of protein synthesis independently of the well-characterized cytoplasmic ribosomes (6,7).…”

The Synthesis of Proteins by the Cytoplasmic Components of Animal Cells*

“…I) the mitochondria are far from being inactive in this respect. This is also true under in vitro conditions as shown by the work, for example, of McLean, Greengard & Campbell (1959) and Reis, Coote & Work (1959). These authors have all shown that washed mitochondria from rat liver when incubated under suitable conditions, either aerobic or anaerobic, incorporate amino acids into the mitochondrial protein.…”

“…This may be the reason for our inability to show an amino acid effect with microsome preparations. It has recently been found that amino acid-activating enzymes are present in extracts of mitochondria (Reis et al 1959; . Hence in this system too amino acid activation may be a step in protein synthesis.…”

Structure and Function of Mitochondria

“…Stafford (197) Martin & Morton (198) Martin & Morton (199) Akazawa & Beevers (200) Lindblad (70) Kmetec & Newcomb (201) Hanson et al (61) Reis et al (65) studies on the distribution of rr. itochondria or in the localization of enzyme functions.…”