Protein/CaCO3/Chitin Nanofiber Complex Prepared from Crab Shells by Simple Mechanical Treatment and Its Effect on Plant Growth

Aklog, Yihun Fantahun; Egusa, Mayumi; Kaminaka, Hironori; Izawa, Hironori; Morimoto, Minoru; Saimoto, Hiroyuki; Ifuku, Shinsuke

doi:10.3390/ijms17101600

Cited by 48 publications

(27 citation statements)

References 17 publications

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“…lycopersici , the causative agent of Fusarium wilt disease to tomatoes, were suppressed by adding CNF and CNF composites prepared from crab shells to soils [ 7 ]. However, CNF, per se, was an unusable nitrogen source for growing tomato plants [ 8 ]. CNF application at high concentrations strongly induced disease resistance but simultaneously inhibited plant growth, which may be caused by the tradeoff between growth and defense [ 6 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Kaminaka

Miura

Isowa

et al. 2020

Plants

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Chitin, an N-acetylglucosamine polymer, is well-known to have unique biological functions, such as growth promotion and disease resistance induction in plants. Chitin has been expectedly used for improving crop yield using its functions; however, chitin derivatives, such as chitin oligosaccharide (CO) and chitosan, are widely used instead since chitin is difficult to handle because of its insolubility. Chitin nanofiber (CNF), produced from chitin through nanofibrillation, retains its polymeric structure and can be dispersed uniformly even in water. Here, the effects of CO and CNF on plant responses were directly compared in soybeans (Glycine max) to define the most effective method to produce chitin derivatives for plant response induction. The growth promotion of aerial parts was observed only in CNF-treated plants. The transcriptome analysis showed that the number of differentially expressed genes (DEGs) in CNF-treated soybeans was higher than in CO-treated soybeans. Notably, the expression patterns of DEGs were mostly similar but were strongly induced by CNF treatment as compared with the CO group. These results reveal that CNF can induce stronger plant response to chitin than CO in soybeans, suggesting nanofibrillation, rather than oligomerization, as a more effective method to produce chitin derivatives for plant response induction.

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Section: Introductionmentioning

confidence: 99%

Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Kaminaka

Miura

Isowa

et al. 2020

Plants

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“…Therefore, protein/CaCO 3 /chitin nanofiber complex was successfully obtained from red snow crab shells ( Figure ). [ 66 ] Some scholars [ 67 ] applied the grinding machine to the pretreatment of chitin and studied its effect on the preparation of chitin nanofibers from crab shell powder by the HPWJ system. The crushed chitin can be effectively decomposed into nanofibers after being treated by the HPWJ system, which reduces the treatment cycle of the HPWJ system.…”

Section: Preparation Methods Of Nano‐chitinmentioning

confidence: 99%

Recent Progress in Preparation and Application of Nano‐Chitin Materials

Yang

Liu

Pei

et al. 2020

Energy & Environ Materials

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Chitin is a kind of natural macromolecule material which was first discovered in mushrooms and was widely found in the shells of crustaceans and arthropods, the cell walls of fungi (yeast and mold) and algae, and the mollusks. The original chitin in nature usually has an antiparallel molecular chain alignment forming nanofibers connected by inter-and intramolecular hydrogen bonds. These microfibers consist of nanofibers about 2-5 nm in diameter, and about 300 nm long, embedded by protein matrices. Due to their unique dimensional, optical, mechanical, and other characteristics, the preparation of nano-chitin materials is an important subject. It is possible to extract nano-chitins from their sources with various methods, including acid hydrolysis, mechanical disintegration, TEMPO-mediated oxidation, electrospinning, and others. In this article, the latest progress in recent years in the preparation and applications of nano-chitin were reviewed. The morphology of the nano-chitins obtained from the above methods was presented. The advantages and disadvantages of each method were analyzed. An overview of applications of nano-chitins was discussed, including biomedicine, food applications, water treatment, green electronic materials, enzyme immobilization carriers, cotton textile materials, cosmetics, and others. REVIEW Nano-Chitin Materials

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“…The extraction of chitin from crustacean shells is well documented in the literature [6], [16], [18][19][20][21][22]. Extraction consists of three major steps; demineralisation, deproteinisation and depigmentation.…”

Section: A Extractionmentioning

confidence: 99%

Improving Extraction Processes Of Crustacean Chitin Using Solid State Analytical Techniques

F.Ó¹

2019

IJAC

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Solid state analytical techniques are becoming more widely used for the analysis of a range of organic products which demonstrate very poor solubility in both common organic and polar solvents and as such cannot be accurately characterised using solution based techniques. Primarily used as a secondary technique for qualitative analysis of insoluble intermediates and products in organic synthesis, 13 C CP-MAS NMR can be utilised in tandem with a targeted extraction and clean up procedure for accurate quantitative analysis of insoluble bio-molecules of interest. Here solid state 13 C CP-MAS NMR is utilised as the primary analytical technique in the characterisation of crustacean sourced chitin whereby Cancer pagurus crab shell chitin and Pandalus borealis shrimp shell chitin are shown to have a degree of acetylation greater than 90%. FTIR spectroscopy, Raman spectroscopy and DSC provide secondary structural, molecular and thermal analysis of the raw materials and extracted chitin.

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Protein/CaCO3/Chitin Nanofiber Complex Prepared from Crab Shells by Simple Mechanical Treatment and Its Effect on Plant Growth

Cited by 48 publications

References 17 publications

Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Recent Progress in Preparation and Application of Nano‐Chitin Materials

Improving Extraction Processes Of Crustacean Chitin Using Solid State Analytical Techniques

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