Protein CD133 as a tumor stem cell marker

Grabovenko, F. I.; Kisil, О. V.; Pavlova, Galina; Zvereva, Maria I.

doi:10.17116/neiro202286061113

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…As one of the classical markers, CD133 has been identified in CSCs of various cancer tissues, and plays a crucial role in tumor spread, cell proliferation, apoptosis evasion and chemoresistance. 28 In addition, CD44 is also critical for maintenance of CSCs stemness, and promotes malignancy progression including migration, invasion and tolerance to chemoradiotherapy. 29 Here, we found that PC decreased the expressions of CD44 and CD133 in CRC cells, suggesting that PC inhibited CCSCs stemness.…”

Section: Discussionmentioning

confidence: 99%

“…Stem cell surface markers can be used to identify and isolate CSCs such as CD44, and CD133. As one of the classical markers, CD133 has been identified in CSCs of various cancer tissues, and plays a crucial role in tumor spread, cell proliferation, apoptosis evasion and chemoresistance 28 . In addition, CD44 is also critical for maintenance of CSCs stemness, and promotes malignancy progression including migration, invasion and tolerance to chemoradiotherapy 29 .…”

Section: Discussionmentioning

confidence: 99%

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Proanthocyanidins inhibited colorectal cancer stem cell characteristics through Wnt/β‐catenin signaling

Chen

Yang

et al. 2023

Environmental Toxicology

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BackgroundCancer stem cells (CSCs) play a key role in tumor cell growth, drug resistance, recurrence, and metastasis. Proanthocyanidins (PC) is widely existed in plants and endowed with powerful antioxidant and anti‐aging effects. Interestingly, recent studies have found that PC exhibits the inhibitory effect on tumor growth. However, the role of PC in CSCs of colorectal cancer (CRC) and molecular mechanism remain unclear.MethodsCCK‐8, colony, and tumorsphere formation assay were used to evaluate cancer cell viability and stemness, respectively. Western blotting was used to detect the protein expression. Tumor xenograft experiments were employed to examine the tumorigenicity of CRC cells in nude mice.ResultsPC decreased the proliferation of CRC cells (HT29 and HCT‐116), and improved the sensitivity of CRC cells to oxaliplatin (L‐OHP), as well as inhibited tumor growth in nude mice. Further studies showed that PC also down‐regulated CSCs surface molecular and stemness transcriptional factors, while suppressed the formations of tumorspheres and cell colony in CRC. In addition, PC‐impaired proteins expressions of p‐GSK3β, β‐catenin and DVL1‐3. LiCl, an activator of the Wnt/β‐catenin signaling, rescued PC‐induced downregulation of CSCs markers, and reduction of tumorspheres and cell colony formation abilities in CRC cells. Furthermore, the effects of PC on inhibiting cell proliferation and enhancing L‐OHP sensitivity were impaired by LiCl.ConclusionsPC exerted an inhibitory effect on CSCs via Wnt/β‐catenin in CRC, and may be a potential new class of natural drug for CRC treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proanthocyanidins inhibited colorectal cancer stem cell characteristics through Wnt/β‐catenin signaling

Chen

Yang

et al. 2023

Environmental Toxicology

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CD133 ligand‐enhanced etoposide‐liposome complex for targeted killing of lung cancer cells

Nie,

Zhou,

Zheng

et al. 2024

Biotechnology Progress

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Lung cancer has a high incidence rate and a low cure rate, hence the urgent need for effective treatment methods. Current lung cancer drugs have several drawbacks, including low specificity, poor targeting, drug resistance, and irreversible damage to normal tissues. Therefore, there is a need to develop a safe and effective new drug that can target and kill tumor cells. In this study, we combined nanotechnology and biotechnology to develop a CD133 ligand‐modified etoposide‐liposome complex (Lipo@ETP‐CD133) for targeted therapy of lung cancer. The CD133 ligand targeted lung cancer stem cells, causing the composite material to aggregate at the tumor site, where high levels of ETP liposomes could exert a strong tumor‐killing effect. Our research results demonstrated that this nano‐drug had efficient targeting and tumor‐killing effects, indicating its potential for clinical application.

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Experience in creating primary cultures of endometrial cancer and studying cells carrying phenotype of cancer stem cells

Filippova,

Mezhevova,

Chembarova

et al. 2024

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Purpose of the study. Was to investigate the possibility of applying the method of spheroid formation in culture for assessment of the endometrial cancer (EC) tumor stem cells (TSC) content in complex samples containing various tumor cells and microenvironment.Materials and methods. Primary cultures were obtained from fragments of tumors removed during surgery as a first stage of treatment at the Department of Gynecological Oncology, the National Medical Research Center for Oncology. After enzymatic disaggregation of tissue, cell suspension was passaged in DMEM medium containing 10 % fetal bovine serum and 1 % gentamicin to obtain primary two-dimensional cultures. To study the ability of cells to form spheroids, the primary culture was removed from the culture plate and passaged with 2.0 × 104 cells per well of a six-well plate (n = 6) in DMEM medium containing 0.35 % agarose and growth factors EGF (20 ng/ml) and FGF (20 ng/ml). After two weeks of cultivation, the average size, number of formed spheroids, and frequency of spheroid formation were determined. For those cultures that had formed spheroids, immunofluorescent staining of the two-dimensional culture for the marker CD133 was performed, after which the frequency of CD133+ cells was determined.Results. A total of nine primary cultures of EC were obtained, five of which formed spheroids within two weeks of cultivation under non-adhesive conditions. In these cultures, small polygonal CD133+ cells showed the strongest association with spheroid formation, which were associated with the largest spheroids (98–110 μm in diameter).Conclusion. There is a large number of microenvironmental cells in mixed cultures of CSC, some of which may express CD133, including healthy stem cells that also form spheroids in soft agar. A more detailed study of CSC subpopulations compared to normal endometrium is required to establish a link between the observed diversity of cells in culture and their ability to form spheroids and other characteristics of tumor stem cells.

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Protein CD133 as a tumor stem cell marker

Cited by 3 publications

References 47 publications

Proanthocyanidins inhibited colorectal cancer stem cell characteristics through Wnt/β‐catenin signaling

Proanthocyanidins inhibited colorectal cancer stem cell characteristics through Wnt/β‐catenin signaling

CD133 ligand‐enhanced etoposide‐liposome complex for targeted killing of lung cancer cells

Experience in creating primary cultures of endometrial cancer and studying cells carrying phenotype of cancer stem cells

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