Protein chip for the parallel quantification of high and low abundant biomarkers for sepsis

Sauer, Ursula; Domnanich, Patrick; Preininger, Claudia

doi:10.1016/j.ab.2011.07.038

Cited by 36 publications

(25 citation statements)

References 17 publications

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“…Procalcitonin (PCT), which is a novel marker of inflammation and infection, has been widely investigated for its prognostic value in septic patients. Although studies have produced conflicting results [30][31][32][33][34][35][36], Tasdelen Fisgin et al [30] and Hensler et al [32] demonstrated that neopterin was a better prognostic factor than PCT in patients with sepsis.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

Bavunoğlu

Genc

Konukoğlu

et al. 2016

J Infect Dev Ctries

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Introduction: Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis. Methodology: Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated. NOx, GSH levels and PON1 activity were determined by colorimetric methods, whereas neopterin, VCAM, ICAM, IL-1, IL-6, TNF-α, NT, and oxLDL levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: All parameters in mild and severe sepsis were significantly different from those of healthy subjects, except ADA activities. Patients with severe sepsis exhibited higher IL-6, TNF-α, NT, and oxLDL levels than patients with mild sepsis. GSH (98%, 98%), oxLDL (98%, 98%), VCAM-1 (99%, 99%), and ICAM-1 (99%, 99%) have much more sensitivitiy and specificity in sepsis. Conclusions: Our results suggest that the oxidative stress and inflammatory response in patients with sepsis were increased and that serum IL-6, TNF-α, NT, and oxLDL levels were correlated with the severity of sepsis. Therefore, increases in these parameters may contribute to the dysfunction or failure of one or more organs, or even death, in sepsis.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

Bavunoğlu

Genc

Konukoğlu

et al. 2016

J Infect Dev Ctries

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“…The chip platforms used were: ARChip epoxy slides (EP 02799374; US 10/490543) [15], aldehyde slides from Arrayit (USA) and poly-L-lysine slides from PolyAn (Germany). Sodium dodecyl sulfate (SDS), CaCl 2 , NaCl, N-hydroxysuccinimide (NHS) and adipic acid dihydrazide (AADH) were from Sigma-Aldrich (USA), as well as: bovine serum albumin (BSA), mariculture keyhole-limpet-hemocyanine (KLH), alkaline phosphatase (ALP) and the CF647 M a n u s c r i p t labelled detection antibody (anti-rabbit IgG).…”

Section: Materials and Reagentsmentioning

confidence: 99%

Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk

Poller

Crooks

Preininger

2015

Sensors and Actuators B: Chemical

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“…Table 1 The assay processing times with the achieved limits of detection reported by Buchegger et al (Buchegger et al, 2012). Another work by Sauer and co-workers (Sauer et al, 2011) reports on the development of another sepsis diagnostic microarray. The authors combined two different assay formats on the same microarray to detect 7 of the low and high abundance sepsis biomarkers.…”

Section: State Of the Art Of Diagnostic Microarraysmentioning

confidence: 99%

The Impact of Diffusion on Biochemical Assay Kinetics in 3d-Hydrogel Microarrays

Mahmoud¹

2015

JOSHA

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JOSHA is a service that helps scholars, researchers, and students descover, use, and build upon a wide range of content DeclarationI hereby confirm that this master thesis at hand is solely my own written work and that no other sources, than those indicated in the references, were used.All text passages and diagrams, quoted or paraphrased, from these sources have been properly acknowledged as such and fully cited.This thesis has not been submitted in the same, similar or even partial version to another examination board and was not published elsewhere. In the past few years, this technology has developed a lot with the implementation of novel surface chemistries, detection techniques, and different assay formats. 3D-hydrogel microarrays were developed using unique immobilization techniques based on hydrophilic polymer networks. The 3D polymer network attaches and immobilizes the capture molecules onto the surface in form of a spot with molecules immobilized both on the surface and inside the gel matrix. This retains the molecule's natural confirmation and structure and enables higher immobilization efficiency, which allows for higher sensitivity. However, the achieved sensitivities are still far from the theoretical limit and in many cases, long incubation times are required. These limitations are caused by both the resolution of the used detection techniques and the assay kinetics. This hinders the transfer of the technology from the laboratory to routine diagnostics.The kinetics of a biochemical assay in a microarray can be controlled either by the transport of molecules to the spot or by the binding interaction itself. This work focuses on studying the assay kinetics in 3D-hydrogel microarrays in order to understand and characterize the limiting step for signal development. To simulate conditions of high affinity binding partners and therefore reduce the influence of the reaction kinetics, the biotin-streptavidin system was selected as the biological model for this work.A microarray to study the kinetic processes involved in the signal development was designed and the optimum working concentrations for kinetic characterization were defined. To confirm the mass transport limited kinetics, the measured kinetics was compared to the ideal reaction kinetics depending on the affinity parameters for biotin-streptavidin interaction. The ideal reaction kinetics was three orders of magnitude faster than measured kinetics. The two-compartment model, which is widely used to describe the assay kinetics in 2D microarrays, was used to fit the observed kinetics. However, a deviation from the model after the initial phase of signal development was observed. A hypothesis was made that this deviation is due to an additional diffusion step in the hydrogel. Therefore, a microarray model to study this diffusion step was designed. In this model, the microarrays were dip coated with hydrogel layers of various thickness and mesh sizes. This model simulated conditions where the signal development should depend only on the diffusion t...

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Protein chip for the parallel quantification of high and low abundant biomarkers for sepsis

Cited by 36 publications

References 17 publications

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk

The Impact of Diffusion on Biochemical Assay Kinetics in 3d-Hydrogel Microarrays

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