Protein digestibility and bioavailability in an F2 mouse cross between the selected fat mouse line and an M2 congenic line carrying the anti‐obesity and anti‐diabetic Tst allele

Abstract

The mouse is an important genetic model for the study of obesity and other human diseases (Bunger et al., 1999; Rudrapatna et al., 2019). Obesity is a major risk factor for several chronic disorders, including diabetes, cardiovascular diseases and cancer. It is a complex trait that is affected by various genetic and interacting environmental factors (Prevoršek et al., 2010). Our previous positional cloning study identified a causal gene for the Fob3b2 QTL on mouse chromosome 15 that confers anti-obesity effects in a polygenic mouse

Genome-wide screening for genetic variants in polyadenylation signal (PAS) sites in mouse selection lines for fatness and leanness

“…Meanwhile, in the Lean line, the core genes of the disease network are Nf2 (neurofibromin 2), Lrp1 (low-density lipoprotein receptor-related protein 1) and Ifngr2 (interferon gamma receptor 2), which we found here to be differentially expressed (higher in the WAT of Fat line), but the PAS-SNPs do not seem to have any influence here on the APA. Nevertheless, IFNGR2 is part of the interferon-γ receptor complex involved in the JAK (Janus kinase)/STAT (signal transducer and activator of transcription) signalling pathway, which may contribute to adipocyte dysfunction and insulin resistance (Gurzov et al 2016 ), the latter possibly occurring in our Fat line (Pirman et al 2021 ). In addition, the expression level of Lat (linker for activation of T cells), which carries a priority candidate PAS-SNP rs33079159 in the Lean line, was lower in the Fat line WAT.…”