Protein digestomics: Integrated platforms to study food-protein digestion and derived functional and active peptides

Picariello, Gianluca; Mamone, Gianfranco; Nitride, Chiara; Addeo, Francesco; Ferranti, Pasquale

doi:10.1016/j.trac.2013.08.001

Cited by 82 publications

(44 citation statements)

References 159 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…. Dynamic models represent a novel approach and have significant advantages over static models, including accounting for the effects of peristalsis and transition of proteins between different compartments of the gastrointestinal tract (Dupont & Mackie, 2015;Picariello, Mamone, Nitride, Addeo, & Ferranti, 2013). Both static and dynamic models neglect specific issues such as correlation with in vivo models, accounting for different digestion times in individual compartments depending on type of food, predigestion processes in the mouth, and, very significantly, "matrix effects" (McClements et al, 2015;Rein et al, 2013).…”

Section: Prediction Of Peptide Release Stability and Activitymentioning

confidence: 99%

Meta‐Analysis for Correlating Structure of Bioactive Peptides in Foods of Animal Origin with Regard to Effect and Stability

Maestri

Pavlićević

Montorsi

et al. 2018

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Amino acid (AA) sequences of 807 bioactive peptides from foods of animal origin were examined in order to correlate peptide structure with activity (antihypertensive, antioxidative, immunomodulatory, antimicrobial, hypolipidemic, antithrombotic, and opioid) and stability in vivo. Food sources, such as milk, meat, eggs, and marine products, show different frequencies of bioactive peptides exhibiting specific effects. There is a correlation of peptide structure and effect, depending on type and position of AA. Opioid peptides contain a high percentage of aromatic AA residues, while antimicrobial peptides show an excess of positively charged AAs. AA residue position is significant, with those in the first and penultimate positions having the biggest effects on peptide activity. Peptides that have activity in vivo contain a high percentage (67%) of proline residues, but the positions of proline in the sequence depend on the length of the peptide. We also discuss the influence of processing on activity of these peptides, as well as methods for predicting release from the source protein and activity of peptides.

show abstract

Section: Prediction Of Peptide Release Stability and Activitymentioning

confidence: 99%

Meta‐Analysis for Correlating Structure of Bioactive Peptides in Foods of Animal Origin with Regard to Effect and Stability

Maestri

Pavlićević

Montorsi

et al. 2018

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…The Yield of Peptides. [9,10] The preparation of standard curve (casein), Biuret method is to determine the rate of peptide. Samples analysis: The sample solution 5 ml combined with 5 ml20 % of TCA shocked 10 s and stood for 10 min, centrifuged 20 min under 8000 r/min, separated out clear liquid.…”

Section: Methodsmentioning

confidence: 99%

The Research of Small Molecule Soybean Polypeptide Precipitated by TCA

Xiong¹,

Xiang²,

Ye³

et al. 2016

Proceedings of the 2016 International Conference on Biological Sciences and Technology

View full text Add to dashboard Cite

Abstract. Trichloroacetic acid (tca) makes macromolecular peptides expose more hydrophobic groups and have precipitation effect. It's widely used in the desalination and concentration of protein and peptide. This experiment use the alkaline protease enzymolysis the soybean protein isolated and makes the products as raw material, then do the high performance liquid chromatography (hplc) analysis of tca precipitation before and after enzymolysis liquid. Macromolecular peptidesore more than 10000 completely precipitated. Results show that the precipitation rate of more than 3000 u reached 62.01%, the molecular weight of 3000-1000 u is about 37.71%.When the molecular weight is less than 1000 u, the polypeptide precipitation rate drops to 22.51%. For the peak belonging 420 u, the main reason is that tca have strong absorption peak and a small amount of impurity absorption peak.

show abstract

“…Both studies give an overall view of the peptidic profile generated in vivo, in which bioactive peptides were particularly tracked. The application of MS-based techniques on the formation of milk peptides, especially bioactive ones, and their changes throughout digestion has been recently reviewed (Picariello et al 2013;Sanchez-Rivera et al 2014).…”

Section: Diversity Of the In Vivo Studiesmentioning

confidence: 99%

On the trail of milk bioactive peptides in human and animal intestinal tracts during digestion: A review

Boutrou

Henry

Sánchez-Rivera

2015

Dairy Sci. & Technol.

View full text Add to dashboard Cite

Digestion of proteins leads to the release of numerous peptides in the gastrointestinal tract, among them several bioactive peptides. This review compiles, organises and critically analyses the information available on bioactive peptides present in vivo in the digestive tract of adult humans and animals. It focuses on milk proteins, which are recognised as one of the main sources of bioactive peptides with different biological functions. To date, few studies have been performed on the presence of milk-derived peptides in the intestinal tract in vivo. The diversity of these studies is remarkable in terms of the milk products that have been tested, the animal models selected and the segment of the digestive tract considered. Altogether, 69 bioactive peptides identified in vivo are reviewed. Most of them are released from casein, and especially from β-casein, to which 38 bioactive peptides are attributed. These sequences can be classified mainly as angiotensin-converting enzyme (ACE) inhibitors, opioids and antimicrobial peptides. Particular attention is paid to βcasomorphins and caseinomacropeptide-derived peptides. The function of caseinophosphopeptides as a mineral carrier is also discussed. Few bioactive peptides derive from whey proteins.

show abstract

Protein digestomics: Integrated platforms to study food-protein digestion and derived functional and active peptides

Cited by 82 publications

References 159 publications

Meta‐Analysis for Correlating Structure of Bioactive Peptides in Foods of Animal Origin with Regard to Effect and Stability

Meta‐Analysis for Correlating Structure of Bioactive Peptides in Foods of Animal Origin with Regard to Effect and Stability

The Research of Small Molecule Soybean Polypeptide Precipitated by TCA

On the trail of milk bioactive peptides in human and animal intestinal tracts during digestion: A review

Contact Info

Product

Resources

About