Protein disorder–order interplay to guide the growth of hierarchical mineralized structures

Elsharkawy, Sherif; Al-Jawad, Maisoon; Pantano, Maria F.; Tejeda‐Montes, Esther; Mehta, Khushbu; Jamal, Hasan; Agarwal, Shweta; Shuturminska, Kseniya; Rice, Alistair; Tarakina, Nadezda V.; Wilson, Rory M.; Bushby, A. J.; Alonso, Matilde; Rodríguez‐Cabello, José Carlos; Barbieri, Ettore; Hernández, Armando del Río; Stevens, Molly M.; Pugno, Nicola; Anderson, Paul; Mata, Álvaro

doi:10.1038/s41467-018-04319-0

Cited by 135 publications

(167 citation statements)

References 67 publications

(78 reference statements)

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“…At the end of 7 d of incubation, the flake-like crystals fully covered the dentin surface and sealed the DTs in-depth (Figure 5g). [4,5,37,38] For instance, the current clinical products, such as Green Or, Hybrid Coat, Gluma Desensitizer, Duraphat ( Figure S22, Supporting Information), and 3M single bond universal adhesive ( Figure S23, Supporting Information), only covered the dentin surface with zero occluded depth inside the DTs. The energy dispersive X-ray (EDX) result reflected that the Ca/P ratio of the regenerated minerals was 1.72, which was assigned to HAp (Figure 5i).…”

Section: Wwwadvmatde Wwwadvancedsciencenewscommentioning

confidence: 99%

Amyloid‐Like Rapid Surface Modification for Antifouling and In‐Depth Remineralization of Dentine Tubules to Treat Dental Hypersensitivity

Deng

et al. 2019

Advanced Materials

114

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occluding resins, dentin adhesives, [3] mineral nanoparticles, or remineralization coatings, such as commercially available Green Or, Hybrid Coat, Gluma Desensitizer, and Duraphat, as well as polyphenol-based coatings, have been proposed for DH treatment. [4][5][6] Unfortunately, the majority of existing desensitizers fail to obtain satisfactory results for treating DH because they only seal orifices within 10 µm of DTs instead of a deep occlusion, and these sealers tend to peel off due to mechanical friction (such as tooth brushing). With the mineralizing adhesives as example, they typically seal the dentin surface instead of in-depth of DTs. Moreover, the leaching of unpolymerized monomers from adhesives has been implicated in hypersensitivity, cytotoxicity, genotoxicity, estrogenicity, and alteration of immune responses. [7] On the other hand, the antifouling property has been ignored for a long time in existing desensitizing products, and as a result, oral bacteria easily adhere onto the salivary acquired pellicle (SAP)-coated mineral layer to form a biofilm, [8] which produce toxins and even cause chronic diseases such as pulpitis. To overcome the drawbacks of current products, developing a rapid and convenient occlusion technique in deep DTs combining antifouling and easy remineralization is necessary but challenging to treat DH with long-term curing stability.Recently, our work demonstrated that lysozyme could undergo a fast amyloid-like aggregation in physiological conditions through the rapid reduction of its intramolecular disulfide bonds by tris(2-carboxyethyl)phosphine (TCEP). [9] In this process, the high-energy α-helix structure of native lysozyme is unfolded and aggregates into β-sheet stacking-based oligomers. A protein nanofilm, also called phase-transitioned lysozyme (PTL), is then formed in ≈2 h through the agglomeration of the oligomers at the liquid/solid interface, offering a robust, multifunctional, biocompatible and colorless transparent coating on flat or porous macroscopic substrates as well as micro/nanoparticles. [10,11] In the present study, we report a greatly deep occlusion of DTs by coating DTs with antifouling amyloid-like lysozyme oligomer aggregates and occluding with in situ formation of a biomimetic hydroxyapatite (HAp) layer on the coating. We demonstrate that the oligomer species, Exposure of dentinal tubules (DTs) leads to the transmission of external stimuli within the DTs, causing dental hypersensitivity (DH). To treat DH, various desensitizers have been developed for occluding DTs. However, most desensitizers commercially available or in development are only able to seal the orifices, rather than the deep regions of the DTs, thus lacking long-term stability. Herein, it is shown that the fast amyloid-like aggregation of lysozyme (lyso) conjugated with poly(ethylene glycol) (PEG) (lyso-PEG) can afford a robust ultrathin nanofilm on the deep walls of DTs through a rapid one-step aqueous coating process (in 2 min). The resultant nanofilm provides a highly effective antifoul...

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Section: Wwwadvmatde Wwwadvancedsciencenewscommentioning

confidence: 99%

Amyloid‐Like Rapid Surface Modification for Antifouling and In‐Depth Remineralization of Dentine Tubules to Treat Dental Hypersensitivity

Deng

et al. 2019

Advanced Materials

114

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“…In addition, amelogenin, enamel matrix derivative, and gelatin were applied to initiate the growth of aligned apatite nanocrystals on enamel [37][38][39] . Furthermore, the disorder-order interplay of elastinlike recombinamers were exploited to enable highly controlled and hierarchical mineralization resembling enamel 40 .…”

Section: Using Pamam As Nucleation Templates For Dental Remineralizationmentioning

confidence: 99%

Dental remineralization via poly(amido amine) and restorative materials containing calcium phosphate nanoparticles

et al. 2019

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Tooth decay is prevalent, and secondary caries causes restoration failures, both of which are related to demineralization. There is an urgent need to develop new therapeutic materials with remineralization functions. This article represents the first review on the cutting edge research of poly(amido amine) (PAMAM) in combination with nanoparticles of amorphous calcium phosphate (NACP). PAMAM was excellent nucleation template, and could absorb calcium (Ca) and phosphate (P) ions via its functional groups to activate remineralization. NACP composite and adhesive showed acid-neutralization and Ca and P ion release capabilities. PAMAM +NACP together showed synergistic effects and produced triple benefits: excellent nucleation templates, superior acidneutralization, and ions release. Therefore, the PAMAM+NACP strategy possessed much greater remineralization capacity than using PAMAM or NACP alone. PAMAM+NACP achieved dentin remineralization even in an acidic solution without any initial Ca and P ions. Besides, the long-term remineralization capability of PAMAM+NACP was established. After prolonged fluid challenge, the immersed PAMAM with the recharged NACP still induced effective dentin mineral regeneration. Furthermore, the hardness of predemineralized dentin was increased back to that of healthy dentin, indicating a complete remineralization. Therefore, the novel PAMAM+NACP approach is promising to provide long-term therapeutic effects including tooth remineralization, hardness increase, and caries-inhibition capabilities.

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“…poly(L‐lactic acid) (PLLA), polycaprolactone (PCL))13,14 or natural ( exe . collagen, chitosan, alginate)15,16 polymers have been used to promote bone formation as well as peptides17 or proteins18,19 to selectively stimulate relevant processes, such as mineralization. Nonetheless, an ideal bioactive scaffold for maxillary sinus floor reconstruction would enable the possibility to be delivered through minimally invasive means, while rapidly adapting to complex anatomical geometries 20…”

Section: Introductionmentioning

confidence: 99%

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

Okesola

Derkuş

et al. 2020

Adv Funct Materials

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Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.

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Protein disorder–order interplay to guide the growth of hierarchical mineralized structures

Cited by 135 publications

References 67 publications

Amyloid‐Like Rapid Surface Modification for Antifouling and In‐Depth Remineralization of Dentine Tubules to Treat Dental Hypersensitivity

Amyloid‐Like Rapid Surface Modification for Antifouling and In‐Depth Remineralization of Dentine Tubules to Treat Dental Hypersensitivity

Dental remineralization via poly(amido amine) and restorative materials containing calcium phosphate nanoparticles

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

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