2024
DOI: 10.1101/2024.06.03.597171
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Protein Editing using a Concerted Transposition Reaction

Yi Hua,
Nicholas E. S. Tay,
Xuanjia Ye
et al.

Abstract: Protein engineering through the chemical or enzymatic ligation of polypeptide fragments has proven enormously powerful for studying countless biochemical processesin vitro. In general, this strategy necessitates a protein folding step following ligation of the unstructured fragments, a requirement that constrains the types of systems amenable to the approach. Here, we report anin vitrostrategy that allows internal regions of target proteins to be replaced in a single operation. Conceptually, our system is anal… Show more

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