Protein Engineering Applications on Industrially Important Enzymes: Candida methylica FDH as a Case Study

Investigation of The Dimer Interface and Active Site in Pyridoxal 5' Phosphate Enzymes Cystathionine B- Lyase and Cystathionine y- Lyase

“…Hydrogen sulfide Technologies that enable the use of renewable energy sources or reduction in energy consumption are becoming highly sought after to address environmental issues, such as global warming (Ordu et al, 2012;Keleş and Bilgen, 2012). This has given rise to the investigation of methods to reduce industry dependence on fossil fuels, and one way of accomplishing this is to increase the energy efficiency of manufacturing and industrial processes.…”

“…by reducing the need to heat reactions to the high temperatures typical of synthetic organic chemistry) and environmental impact (e.g. by eliminating the need for toxic metals as catalysts and organic solvents), while concomitantly increasing the yields of processes that require chemical synthesis or transformations (Ordu et al, 2012). Examples of industrial applications of enzymes include agri-food, pharmaceutical, pulp and paper, cosmetics, sanitation, textiles and many others.…”

“…The efficiency and exquisite substrate and reaction specificity of enzymes makes them attractive tools for efforts to increase productivity while reducing the energy requirements and environmental cost of manufacturing or industrial processes involving chemical transformations (Ordu et al, 2012). However, the subset of reactions for which naturally occurring biocatalysts can be found is much smaller than the range of possible chemical transformations, such that enzymes do not currently exist for many industrial applications.…”