Protein Expression Profiles during Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

Kim, Sollip; Min, Won‐Ki; Chun, Sail; Lee, Woochang; Chung, Hee-Jung; Choi, Soo Jin; Yang, Scott; Yang, Yoon Sun; Yoo, Jong-Il

doi:10.1620/tjem.221.141

Cited by 15 publications

(10 citation statements)

References 20 publications

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“…During this process, tubule cells loose epithelial features and convert to fibroblast-like cells (Guarino et al 2009). The epithelialmesenchymal transition (EMT) is paralleled by the expression of fibroblast-specific protein-1(FSB1) (Kim et al 2010), heat shock proteins and a-smooth muscle actin (Abe et al 2000;Lim et al 2009;Vidyasagar et al 2008). Information about the origin of interstitial cells is barely available.…”

Section: Renal Interstitiummentioning

confidence: 99%

Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche

Minuth

Denk

Miess

et al. 2011

Histochem Cell Biol

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The development of the nephron is piloted by interactions between epithelial and surrounding mesenchymal stem/progenitor cells. Data show that an astonishingly wide interstitial space separates both kinds of stem/progenitor cells. A simple contrasting procedure was applied to visualize features that keep renal epithelial and mesenchymal stem/progenitor cells in distance. The kidney of neonatal rabbits was fixed in solutions containing glutaraldehyde (GA) in combination with alcian blue, lanthanum, ruthenium red, or tannic acid. To obtain a comparable view to the renal stem/progenitor cell niche, the tissue was exactly orientated along the axis of collecting ducts. Fixation with GA or in combination with alcian blue or lanthanum revealed an inconspicuous interstitial space. In contrast, fixation with GA containing ruthenium red exhibits strands of extracellular matrix lining from epithelial stem/progenitor cells through the interstitium up to the surface of mesenchymal stem/progenitor cells. Fixation with GA containing tannic acid shows that the basal lamina of epithelial stem/progenitor cells, the adjacent interstitial space and also the surface of mesenchymal stem/progenitor cells are connected over a net of extracellular matrix. The applied technique appears to be a suitable method to illuminate the interstitium in stem/progenitor cell niches of specialized tissues, the microenvironment of tumors and extension of degeneration.

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Section: Renal Interstitiummentioning

confidence: 99%

Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche

Minuth

Denk

Miess

et al. 2011

Histochem Cell Biol

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“…However, these studies are limited in the number of proteins identified because of the nonglobal nature of these approaches and the quantification of only known factors. Recently, quantitative proteomics studies of MSC obtained from a variety of sources have been reported using a 2D-gel approach where only the differentially expressed proteins are analyzed (10,11). In addition, mass spectrometry-based secretome analyses of human MSC derived from a number of tissues have been reported (8,(12)(13)(14)(15).…”

mentioning

confidence: 99%

Temporal Profiling and Pulsed SILAC Labeling Identify Novel Secreted Proteins During Ex Vivo Osteoblast Differentiation of Human Stromal Stem Cells

Kristensen

Chen

Nielsen

et al. 2012

Molecular & Cellular Proteomics

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It is well established that bone forming cells (osteoblasts) secrete proteins with autocrine, paracrine, and endocrine function. However, the identity and functional role for the majority of these secreted and differentially expressed proteins during the osteoblast (OB) differentiation process, is not fully established. To address these questions, we quantified the temporal dynamics of the human stromal (mesenchymal, skeletal) stem cell (hMSC) secretome during ex vivo OB differentiation using stable isotope labeling by amino acids in cell culture (SILAC). In addition, we employed pulsed SILAC labeling to distinguish genuine secreted proteins from intracellular contaminants. We identified 466 potentially secreted proteins that were quantified at 5 time-points during 14-days ex vivo OB differentiation including 41 proteins known to be involved in OB functions. Among these, 315 proteins exhibited more than 2-fold up or down-regulation. The pulsed SILAC method revealed a strong correlation between the fraction of isotope labeling and the subset of proteins known to be secreted and involved in OB differentiation. We verified SILAC data using qRT-PCR analysis of 9 identified potential novel regulators of OB differentiation. Furthermore, we studied the biological effects of one of these proteins, the hormone stanniocalcin 2 (STC2) and demonstrated its autocrine effects in enhancing osteoblastic differentiation of hMSC. In conclusion, combining complete and pulsed SILAC labeling facilitated the identification of novel factors produced by hMSC with potential role in OB differentiation. Our study demonstrates that the secretome of osteoblastic cells is more complex than previously reported and supports the emerging evidence that osteoblastic cells secrete proteins with endocrine functions and regulate cellular processes beyond bone formation. Molecular & Cellular

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“…35 During this process, tubule cells loose epithelial features and convert to fibroblast-like cells. 36 Although under actual debate, 37 the epithelial-mesenchymal transition (EMT) is paralleled by the expression of fibroblast-specific protein-1(FSB1), 38 heat shock proteins, and a-smooth muscle actin. [39][40][41] When stem/progenitor cells are implanted into a diseased kidney, they have to concentrate at sites with damaged parenchyma.…”

Section: Discussionmentioning

confidence: 99%

Interstitial interfaces show marked differences in regenerating tubules, matured tubules, and the renal stem/progenitor cell niche

Minuth

Denk

2012

J Biomedical Materials Res

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Stem/progenitor cells are promising candidates for the regeneration of parenchyma in acute and chronic renal failure. After an implantation stem/progenitor cells must migrate through the interstitial space to concentrate at the site of damage. However, information is lacking to what extent the interstitial interface is influencing the development of stem/progenitor cells into nephron structures. In consequence, tubule regeneration within an artificial polyester interstitium was analyzed by electron microscopy in comparison with the interstitial interface of matured tubules and the interstitium within the renal stem/progenitor cell niche. The experiments demonstrate that fixation of specimens with glutaraldehyde (GA) is leading in all cases to inconspicuously looking interstitial interfaces. In contrast, fixation of regenerating tubules in GA containing ruthenium red and tannic acid shows a dense network of fibers lining along the basal lamina. In contrast, matured tubules reveal after ruthenium red label an extremely thickened basal lamina, while only a punctate pattern is obtained after tannic acid treatment. Finally, within the renal stem/progenitor cell niche ruthenium red and tannic acid label reveals large amounts of extracellular matrix spanning through the interstitium. Thus, fixation of tissue in GA containing ruthenium red and tannic acid exhibits an unexpectedly regional heterogeneity of the renal interstitial interface. This fact has to be considered for an optimal therapeutic repair of parenchyma, since contacts between stem/progenitor cells with the interstitial interface influence further development.

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Protein Expression Profiles during Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

Cited by 15 publications

References 20 publications

Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche

Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche

Temporal Profiling and Pulsed SILAC Labeling Identify Novel Secreted Proteins During Ex Vivo Osteoblast Differentiation of Human Stromal Stem Cells

Interstitial interfaces show marked differences in regenerating tubules, matured tubules, and the renal stem/progenitor cell niche

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