Protein extraction and sludge dewatering performance of ultrasound-assisted enzymatic hydrolysis of excess sludge

Yan, Yixin; Qin, Lei; Gao, Jianlei; Nan, Ruiqi

doi:10.1007/s11356-020-08208-2

Cited by 23 publications

(10 citation statements)

References 35 publications

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“…According to the results of the previous sludge protein extraction test, ATH (Li, 2017), EH (Yan et al, 2020)and UEH (Miao, 2017) under optimized conditions were used to extract sludge protein. The speci c operation process is shown in Fig.…”

Section: Hydrolysis Methods Of Excess Sludgementioning

confidence: 99%

“…Biological methods mainly involve enzymatic hydrolysis (EH) with alkaline protease, which has mild extraction conditions without secondary pollution, but the extraction rate is low (Li et al, 2011;Zhang et al, 2012). Among combined methods, ultrasonic-assisted enzymatic hydrolysis (UEH) can achieve a relatively high extraction rate with low energy consumption (Yan et al, 2020). These three methods are representative methods of sludge protein extraction.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of methods for detecting protein extracted from excess activated sludge

Yan

Zhang

Gao

et al. 2023

Environ Sci Pollut Res

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Currently, the protein content of hydrolyzed sludge supernatant is commonly determined by the Kjeldahl method, but this method suffers from complicated operation, a long time requirement and large chemical consumption. In this paper, the Lowry, bicinchoninic acid (BCA) and Bradfordmethods were used to test the precision and spiked recovery of protein from sludge supernatant hydrolyzed by alkaline-thermal hydrolysis (ATH), enzymatic hydrolysis (EH) and ultrasound-assisted enzymatic hydrolysis (UEH), and the results were compared with those obtained with the Kjeldahl method. For all the hydrolysis processes, the sludge protein values determined from the three tested methods were within 0.05 of each other, meeting the experimental requirements for accuracy. Both the Lowry and BCA methods had a recovery rate of 95%-105%, while the Bradford method showed a large deviation and was not highly reliable. The three protein determination methods had signi cant differences from the Kjeldahl method (P<0.05). However, the relative deviation between the Kjeldahl and BCA methods was the smallest (3%-5%), followed by those between the Kjeldahl and the Lowry (11%-21%) and Bradford methods (21%-90%), and the causes of the deviation were analyzed according to the protein hydrolysate components and the mechanisms of the different detection methods. On the basis of the above results, the BCA method was chosen as the most appropriate quanti cation method for use in sludge protein extraction, and it was used to analyze the protein content extracted from residual sludge from two sewage treatment plants. The reliability of the method was veri ed, which lays a foundation for the extraction and reclamation of sludge protein.

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Section: Hydrolysis Methods Of Excess Sludgementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of methods for detecting protein extracted from excess activated sludge

Yan

Zhang

Gao

et al. 2023

Environ Sci Pollut Res

Self Cite

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Section: Hydrolysis Methods Of Excess Sludgementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for Detecting Protein Extracted from Excess Activated Sludge

Yan

Zhang

Gao

et al. 2023

Preprint

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Currently, the protein content of hydrolyzed sludge supernatant is commonly determined by the Kjeldahl method, but this method suffers from complicated operation, a long time requirement and large chemical consumption. In this paper, the Lowry, bicinchoninic acid (BCA) and Bradfordmethods were used to test the precision and spiked recovery of protein from sludge supernatant hydrolyzed by alkaline-thermal hydrolysis (ATH), enzymatic hydrolysis (EH) and ultrasound-assisted enzymatic hydrolysis (UEH), and the results were compared with those obtained with the Kjeldahl method. For all the hydrolysis processes, the sludge protein values determined from the three tested methods were within 0.05 of each other, meeting the experimental requirements for accuracy. Both the Lowry and BCA methods had a recovery rate of 95%-105%, while the Bradford method showed a large deviation and was not highly reliable. The three protein determination methods had significant differences from the Kjeldahl method (P<0.05). However, the relative deviation between the Kjeldahl and BCA methods was the smallest (3%-5%), followed by those between the Kjeldahl and the Lowry (11%-21%) and Bradford methods (21%-90%), and the causes of the deviation were analyzed according to the protein hydrolysate components and the mechanisms of the different detection methods. On the basis of the above results, the BCA method was chosen as the most appropriate quantification method for use in sludge protein extraction, and it was used to analyze the protein content extracted from residual sludge from two sewage treatment plants. The reliability of the method was verified, which lays a foundation for the extraction and reclamation of sludge protein.

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“…Ultrasound treatment can increase the activity of polyphenol oxidase in tea cells during the fermentation process and accelerate the enzymatic oxidation reaction process, allowing the generated flavor substances to overflow smoothly. Factors such as ultrasonic frequency, ultrasonic power density, and ultrasonic time would affect the process of enzymatic oxidation and might influence the process via activation or deactivation due to inadequate conditions [14] , [15] . Therefore, the key to promoting enzymatic reaction with ultrasound is to establish appropriate ultrasound operational modes and process conditions [16] .…”

Section: Introductionmentioning

confidence: 99%

Emerging applications of nano-optical sensors combined with near-infrared spectroscopy for detecting tea extract fermentation aroma under ultrasound-assisted sonication

Kang

Hao

Jiang

et al. 2022

Ultrasonics Sonochemistry

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Protein extraction and sludge dewatering performance of ultrasound-assisted enzymatic hydrolysis of excess sludge

Cited by 23 publications

References 35 publications

Comparison of methods for detecting protein extracted from excess activated sludge

Comparison of methods for detecting protein extracted from excess activated sludge

Comparison of Methods for Detecting Protein Extracted from Excess Activated Sludge

Emerging applications of nano-optical sensors combined with near-infrared spectroscopy for detecting tea extract fermentation aroma under ultrasound-assisted sonication

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