Protein Identification by Database Searching of Mass Spectrometry Data in the Teaching of Proteomics

Marquioni, Vinícius; Nunes, Francis Morais Franco; Novo-Mansur, Maria Teresa Marques

doi:10.1021/acs.jchemed.0c00853

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…In formula (2), ik P represents the comprehensive load of Class I cells, i s represents the load density of the cell in the k-th year. At this time, the predicted load value can be calculated according to the above coordination coefficient α [12], as…”

Section: ()mentioning

confidence: 99%

Method of substation differential power supply zoning based on grid division

Peng,

Deng,

Zhou

et al. 2023

Second International Conference on Energy, Power, and Electrical Technology (ICEPET 2023)

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The conventional substation differential power supply zoning method uses the grey clustering coefficient to determine the power supply weight of each zone, which is vulnerable to the influence of the dynamic entropy value of power supply, leading to the high power supply load. Therefore, a new substation differential power supply zoning method needs to be designed based on grid division. That is, the grid division is used to predict the power supply load of substation partition, and then the differential power supply partition model of substation is constructed by combining the load characteristics complementation, thus realizing the differential power supply partition of substation. The case analysis results show that the designed substation differential power supply zoning method based on grid division has a small power supply load, which proves that the designed power supply zoning method has a good power supply effect, can reduce power supply loss, has a certain application value, and has made a certain contribution to reducing the regional power supply cost.

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Section: ()mentioning

confidence: 99%

Method of substation differential power supply zoning based on grid division

Peng,

Deng,

Zhou

et al. 2023

Second International Conference on Energy, Power, and Electrical Technology (ICEPET 2023)

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“…Recent studies have addressed the lack of teaching proteomics and have facilitated coursework to fill this gap. 18,19 This interdisciplinary field, combining molecular biology with analytical chemistry and bioinformatics, may seem challenging to teach at the graduate level. Yet, we consider that the skill-set of chemists or other majors related to life sciences should envelop emerging technologies into their education, either to prepare them well for graduate studies or for careers in industry.…”

Section: ■ Introductionmentioning

confidence: 99%

Inquiry-based Learning of Proteomics and Metabolomics

Nezhyva,

Aerts,

Sandbaumhüter

et al. 2024

J. Chem. Educ.

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Given the rapid development of data-driven experimental procedures within the life sciences, it is important to equip students with proper skills and knowledge on how to obtain and interpret complex data. While laboratory exercises have for a long time been well established as a teaching method for life sciences, we consider there is room for improvement in how laboratory exercises are conducted. Hence, we designed a laboratory exercise course in which students at the graduate level in the European education system (M.Sc.) are challenged to pose their own biological question and write their own laboratory protocol for a proteomic study to investigate their hypothesis. Here, students are supported in their task with lectures and seminars that take them through the required details on experimental sample preparation, analysis with LC−MS, and proteomic data evaluation of biological function and relevance. According to student interviews, the inquiry-based learning concept we used here provided a deeper understanding of the laboratory protocols they wrote, according to which they eventually performed their own experiments.

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“…There are also several instrumental methods, ranging from gel electrophoresis , to mass spectrometry (MS) methods, ,− which have been used for protein identification and quantification. MS methods are selective and can detect food adulterations with nitrogen-rich compounds .…”

Section: Introductionmentioning

confidence: 99%

Whey Protein Analysis Using the Lowry Assay and 96-Well-Plate Digital Images Acquired Using Smartphones

Assink Junior,

de Jesus,

Borges

2023

J. Chem. Educ.

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Here, students determine the total protein content in whey protein samples using the Lowry assay. Quantitative analysis was carried out using absorbance measured at 750 nm (standard method) and 96-well-plate digital images obtained using smartphones (proposed method). The proposed method was carried out using two smartphones (1 and 2). Smartphone 1 had a higher resolution than smartphone 2. The statistical evaluation of the data set produced by the students was carried out using JASP (Jeffreys's Amazing Statistics Program), and it was a valuable tool to teach descriptive statistics and hypothesis tests. We chose JASP because it is free and open-source software with a graphical user interface. The protein percentage found in five whey protein samples using the standard method during three lab classes was compared using one-way and two-way ANOVA. Five whey protein samples were analyzed using the standard method and the proposed methods (smartphones 1 and 2). The results were compared using one-way repeated measure ANOVA (RMANOVA) and mixed-factor ANOVA. During the statistical analysis of the data set, students identified outlier results using boxplots and checked data normality using the Shapiro−Wilk test, histograms, and boxplots. They learned the difference between dependent and independent variables and compared variances using Levene's test (dependent variables) and Mauchly's test (independent variables). The interpretation of statistical tests was also carried out using descriptives plots, boxplots, and raincloud plots.

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Protein Identification by Database Searching of Mass Spectrometry Data in the Teaching of Proteomics

Cited by 10 publications

References 30 publications

Method of substation differential power supply zoning based on grid division

Method of substation differential power supply zoning based on grid division

Inquiry-based Learning of Proteomics and Metabolomics

Whey Protein Analysis Using the Lowry Assay and 96-Well-Plate Digital Images Acquired Using Smartphones

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