Protein interactions and conformations on graphene-based materials mapped using a quartz-crystal microbalance with dissipation monitoring (QCM-D)

Hampitak, Piramon; Melendrez, Daniel; Iliuţ, Maria; Fresquet, Maryline; Parsons, Natalie; Spencer, Ben F.; Jowitt, Thomas A.; Vijayaraghavan, Aravind

doi:10.1016/j.carbon.2020.04.093

Cited by 71 publications

(64 citation statements)

References 49 publications

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“…Based on our previous paper, a denatured layer of BSA is formed when BSA is adsorbed on Au or highly hydrophobic rGO surface from a low concentration solution. 11 …”

Section: Resultsmentioning

confidence: 99%

“…A controlled thermal treatment of the GO-coated chips was performed in a vacuum oven (Townson+Mercer EV018) with a temperature of 180 °C for 6 h to reduce the GO and obtain a highly hydrophobic rGO coating as described in our previous study. 11 …”

Section: Materials and Methodsmentioning

confidence: 99%

“… 11 , 27 We reported a simple method to obtain a film of denatured BSA (dBSA) on rGO. 11 By physical adsorption of BSA from a low-concentration solution in phosphate-buffered saline (PBS) onto highly hydrophobic rGO at room temperature, a denatured adlayer of BSA can be formed on the rGO. This method is a simple, fast, and cost-effective way to employ dBSA on graphene as an inert biointerface for immobilizing specific protein receptors in graphene biosensors.…”

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confidence: 99%

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A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

et al. 2020

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We present a sensitive and low-cost immunoassay, based on a customized open-source quartz crystal microbalance coupled with graphene biointerface sensors (G-QCM), to quantify antibodies in undiluted patient serum. We demonstrate its efficacy for a specific antibody against the phospholipase A2 receptor (anti-PLA2R), which is a biomarker in primary membranous nephropathy. A novel graphene–protein biointerface was constructed by adsorbing a low concentration of denatured bovine serum albumin (dBSA) on the reduced graphene oxide (rGO) sensor surface. The dBSA film prevents the denaturation of the protein receptor on the rGO surface and serves as the cross-linker for immobilization of the receptor for anti-PLA2R antibodies on the surface. The detection limit and selectivity of this G-QCM biosensor was compared with a commercial QCM system. The G-QCM immunoassay exhibited good specificity and high sensitivity toward the target, with an order of magnitude better detection limit (of 100 ng/mL) compared to the commercial system, at a fraction of the cost and with considerable time saving. The results obtained from patient sera compared favorably with those from enzyme-linked immunosorbent assay, validating the feasibility of use in clinical applications. The multifunctional dBSA-rGO platform provides a promising biofunctionalization method for universal immunoassay and biosensors. With the advantages of inexpensive, rapid, and sensitive detection, the G-QCM sensor and instrument form an effective autoimmune disease screening tool.

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“…Based on our previous paper, a denatured layer of BSA is formed when BSA is adsorbed on Au or highly hydrophobic rGO surface from a low concentration solution. 11 …”

Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

et al. 2020

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“…Moreover, the QCM technique, especially QCM-D, is widely used in cellular characterization [ 42 ] and interactions [ 43 , 44 , 45 ], DNA detection [ 40 , 46 ], and protein detection [ 47 ], as well as high-throughput real-time screening of protein drug targets [ 48 , 49 , 50 ]. Overall, the QCM technology attracts a lot of attention due to the wide variety of possible applications [ 48 , 51 , 52 , 53 ]. The present review is mainly focused on the latest QCM instruments’ achievements in protein and peptide drug product development, with general operations reduced to basic concepts.…”

Section: Qcm Technology Basicsmentioning

confidence: 99%

“…The molecular structure determined by an amino acid sequence has a substantial impact on the properties and activity of peptides and proteins. Apart from quantitative analysis of adsorbed biopolymers, QCM also allows determination of relative surface adsorption affinity, i.e., hydrophobic polystyrene versus hydrophilic silica [ 62 ] or graphene derivatives [ 52 ] .…”

Section: Adsorption Of Peptide and Protein Molecules On The Qcm Sumentioning

confidence: 99%

Application of QCM in Peptide and Protein-Based Drug Product Development

2020

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AT-cut quartz crystals vibrating in the thickness-shear mode (TSM), especially quartz crystal resonators (QCRs), are well known as very efficient mass sensitive systems because of their sensitivity, accuracy, and biofunctionalization capacity. They are highly reliable in the measurement of the mass of deposited samples, in both gas and liquid matrices. Moreover, they offer real-time monitoring, as well as relatively low production and operation costs. These features make mass sensitive systems applicable in a wide range of different applications, including studies on protein and peptide primary packaging, formulation, and drug product manufacturing process development. This review summarizes the information on some particular implementations of quartz crystal microbalance (QCM) instruments in protein and peptide drug product development as well as their future prospects.

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2024

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Protein interactions and conformations on graphene-based materials mapped using a quartz-crystal microbalance with dissipation monitoring (QCM-D)

Cited by 71 publications

References 49 publications

A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

Application of QCM in Peptide and Protein-Based Drug Product Development

Food Applications

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