Protein Interactions on Telomeric Retrotransposons in Drosophila

Takács, S; Biessmann, Harald; Reddy, Hemakumar M.; Mason, James; Török, Tibor

doi:10.7150/ijbs.4460

Cited by 10 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although a previous study had reported that no DREF binding sites were present at the HTT array [10], we identified two DREF binding sites in the TART element of D. melanogaster ; one at the 5′UTR, highly conserved among TART subfamilies, and another at the 3′UTR, only conserved in the TART A subfamily (Figure 2). The high conservation of the 5′UTR DREF binding site, suggests the existence of selective pressure acting at this DNA binding motif.…”

Section: Discussionmentioning

confidence: 69%

“…Interestingly, the TART A subfamily, the one that contains both DREF binding motifs, is the more abundant of the TART subfamilies in D. melanogaster [21]. In addition to the presence of the DREF DNA binding motif in the sequence of the TART element, the recruitment of the DREF/TRF2 complex at the telomeres has been recently demonstrated [10]. …”

Section: Discussionmentioning

confidence: 99%

“…The equilibrium between these proteins is one of the keys to obtaining a precise level of expression of the telomere retrotransposons, HeT-A and TART . A recent study has also confirmed the presence of Pzg at the telomeres when screening for proteins that interact with another component of the HTT array, the Prod protein [9,10]. …”

Section: Introductionmentioning

confidence: 93%

See 2 more Smart Citations

The Putzig partners DREF, TRF2 and KEN are involved in the regulation of the Drosophila telomere retrotransposons, HeT-A and TART

2013

View full text Add to dashboard Cite

BackgroundTelomere maintenance in Drosophila relies on the targeted transposition of three very special non-LTR retrotransposons, HeT-A, TART, and TAHRE (HTT). The sequences of the retrotransposon array build up the telomere chromatin in this organism. We have recently reported the role of the chromosomal protein Putzig/Z4 in maintaining a proper chromatin structure at the telomere domain of Drosophila. Because the Putzig protein has been found in different cellular complexes related with cell proliferation, development, and immunity, we decided to investigate whether the previously described Putzig partners, DREF/TRF2 and KEN, could also be involved in the telomere function in this organism.ResultsWe have found that mutant alleles for Dref/Trf2 and Ken show alterations in HeT-A and TART expression, suggesting a possible role of these protein complexes in the regulation of the telomere retrotransposons. In agreement, both HeT-A and TART contain the specific DNA binding sequences for the DREF and the KEN protein proteins.ConclusionsWe have identified three new negative regulators involved in the control of the expression of the telomeric retrotransposons, Dref, Trf2, and Ken. Our results offer some clues on which other chromatin-related proteins might be involved in telomere regulation and retrotransposon control.

show abstract

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Putzig partners DREF, TRF2 and KEN are involved in the regulation of the Drosophila telomere retrotransposons, HeT-A and TART

2013

View full text Add to dashboard Cite

show abstract

“…Telomeric chromatin consists of the HTT elements and different proteins that are bound to them (Andreyeva et al 2005). The rate of transposition of these HTT elements may depend on an equilibrium between the level of their expression and the chromatin bound proteins (Takács et al 2012;Silva-Sousa et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of the Telomere elongation mutation in Drosophila

Reddy

Randall

Frydrychová

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Background. Telomeres in Drosophila melanogaster are similar to those of other eukaryotes in terms of their function, although they are formed by non-LTR retrotransposons instead of telomerase-based short repeats. The length of the telomeres in Drosophila depends on the number of copies of these transposable elements. A dominant mutation, Tel1, causes a several-fold elongation of telomeres. Methods. In this study we identified the Tel1 mutation by a combination of transposon-induced, site-specific recombination and next generation sequencing. Results. Recombination located Tel1 to a 15 kb region in 92A. Comparison of the DNA sequence in this region with the Drosophila Genetic Reference Panel of wild type genomic sequences delimited Tel1 to a 3 bp deletion inside intron 8 of Ino80. Discussion. The mapped Tel1 mutation (3-bp deletion found in Ino80) did not appear to affect the quantity or length of the Ino80 transcript. Tel1 causes a significant reduction in transcripts of CG18493, a gene nested in an intron 8 of Ino80, which is expressed in ovaries and expected to encode a serine-type peptidase.

show abstract

“…Genome-wide ChIP-sequence analyses also suggest that as well as functioning as a transcription factor DREF, in collaboration with insulator proteins may contribute to maintaining chromosome organization during the cell cycle, marking a subset of genomic sites for the assembly of pre-replication complexes, and gene bookmarking during the M/G1 transition 19 . Furthermore, a DREF/TRF2 complex 20 appears to be important for maintenance of telomere length in Drosophila 21 .…”

mentioning

confidence: 99%

The Hippo pathway as a target of the Drosophila DRE/DREF transcriptional regulatory pathway

Horii

Yanai

et al. 2014

Sci Rep

View full text Add to dashboard Cite

The DRE/DREF transcriptional regulatory system has been demonstrated to activate a wide variety of genes with various functions. In Drosophila, the Hippo pathway is known to suppress cell proliferation by inducing apoptosis and cell cycle arrest through inactivation of Yorkie, a transcription co-activator. In the present study, we found that half dose reduction of the hippo (hpo) gene induces ectopic DNA synthesis in eye discs that is suppressed by overexpression of DREF. Half reduction of the hpo gene dose reduced apoptosis in DREF-overexpressing flies. Consistent with these observations, overexpression of DREF increased the levels of hpo and phosphorylated Yorkie in eye discs. Interestingly, the diap1-lacZ reporter was seen to be significantly decreased by overexpression of DREF. Luciferase reporter assays in cultured S2 cells revealed that one of two DREs identified in the hpo gene promoter region was responsible for promoter activity in S2 cells. Furthermore, endogenous hpo mRNA was reduced in DREF knockdown S2 cells, and chromatin immnunoprecipitation assays with anti-DREF antibodies proved that DREF binds specifically to the hpo gene promoter region containing DREs in vivo. Together, these results indicate that the DRE/DREF pathway is required for transcriptional activation of the hpo gene to positively control Hippo pathways.

show abstract

Protein Interactions on Telomeric Retrotransposons in Drosophila

Cited by 10 publications

References 28 publications

The Putzig partners DREF, TRF2 and KEN are involved in the regulation of the Drosophila telomere retrotransposons, HeT-A and TART

The Putzig partners DREF, TRF2 and KEN are involved in the regulation of the Drosophila telomere retrotransposons, HeT-A and TART

Identification of the Telomere elongation mutation in Drosophila

The Hippo pathway as a target of the Drosophila DRE/DREF transcriptional regulatory pathway

Contact Info

Product

Resources

About