Protein isolates with reduced gossypol content from screw-pressed cottonseed meal

Liadakis, G.; Floridis, Anastasios.; Tzia, Constantina; Oreopoulou, Vassiliki

doi:10.1021/jf00030a016

Cited by 38 publications

(19 citation statements)

References 18 publications

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“…Alkanol:ammonia:water-hexane extraction (Naczk, Myhara, & Shahidi, 1992;Naczk, Shahidi, & Rubin, 1988;Rubin, Diosady, & Phillips, 1984;Tzeng, Diosady, & Rubin, 1990;Wanasundara & Shahidi, 1994) or a series of solvent extraction stages, i.e. butanol, 2-propanol or dichloromethane in 2-4 stages (Liadakis, Floridis, Tzia, & Oreopoulou, 1993), can be performed for removing oligosaccharides and inhibitors such as glucosinolates from canola or gossypol from cottonseed meals or seeds (Gastrock, Eaves, & D'Aquin, 1970). Water is operationally advantageous over alcohols since it is non-flammable, explosive nor toxic.…”

Section: Oilseed Processing Into Protein Productsmentioning

confidence: 99%

Functionality of oilseed protein products: A review

Moure

Sineiro

Domı́nguez

et al. 2006

Food Research International

475

319

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Section: Oilseed Processing Into Protein Productsmentioning

confidence: 99%

Functionality of oilseed protein products: A review

Moure

Sineiro

Domı́nguez

et al. 2006

Food Research International

475

319

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“…Oil absorption index (OAI) was determined according to the method of Liadakis, Floridis, Tzia, and Oreopoulou (1993): refined corn oil (3 mL) was added to sample (0.5 g) in a graduated 15 mL glass centrifuge tube. The tube was agitated on a Vortex mixer for 1 min, left for 30 min and centrifuge for 20 min at 700g; the volume of the free oil was read.…”

Section: Oil Absorption Indexmentioning

confidence: 99%

Functional properties of corn and corn–lentil extrudates

Λάζου

Krokida

2010

Food Research International

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“…Other authors have obtained higher protein concentration in the isolate (94.8%), with sodium hexametaphosphate and acid precipitation from glanded cottonseeds (Shemer, Berk, & Mokady, ). Studies showed that protein isolates had been extracted from glanded cotton seeds with up to 85% protein content (Liadakis, Floridis, Tzia, & Oreopoulou, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Soluble Glandless Cottonseed Meal Proteins Based on Electrophoresis, Functional Properties, and Microscopic Structure

Delgado

Valverde-Quiroz

Lopez

et al. 2019

Journal of Food Science

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This investigation aimed to extract and characterize the GCSM proteins, determine their solubility potential at two different temperatures and different solvents, and explore their functional properties. During the extraction, no water‐ or ethanol‐soluble protein was found. Most of the protein was extracted with KOH solution. GCSM showed major protein bands between 13,273 and 56,564 Da with an isoelectric point of 5.1. The results showed that extraction temperature and solvent affected the amount of protein extracted from GCSM. The highest protein yield (63.4%) was obtained with KOH at 55 °C. Fat content negatively affected the protein solubility. The highest protein purity (99.9%) was obtained with 6% of fat content and the lowest one with 19% of fat content. GCSM has a high glutamic acid content, followed by arginine and aspartic acid compared to the other amino acids. The essential amino acids make up about 30.0% of the total amino acid concentration in KOH‐soluble fractions. The results showed a denaturation temperature of GCSM protein ranging from 61.4 to 63.6 °C. Scanning electron microscopy reveals a microglobular protein structure. GCSM protein isolate showed lower (P < 0.05) water‐holding and oil‐holding capacity but similar gelation properties as soy protein. GCSM protein shows a high foaming capacity at high pH values and high emulsion stability. Practical Application The results of this investigation have a direct impact on the plant protein processing industry. This paper presents a new source of plant protein with a high foaming capacity in alkaline conditions with potential applications for human consumption and feed for aquaculture and animals. The results of this research may impact the cotton producers who can increase their income, and the aquaculture industry will have a cheaper source of protein that can partially substitute the expensive fishmeal. Cottonseed protein can be used to develop high protein extruded snacks and other functional foods, such as plant protein‐based food products.

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Protein isolates with reduced gossypol content from screw-pressed cottonseed meal

Cited by 38 publications

References 18 publications

Functionality of oilseed protein products: A review

Functionality of oilseed protein products: A review

Functional properties of corn and corn–lentil extrudates

Characterization of Soluble Glandless Cottonseed Meal Proteins Based on Electrophoresis, Functional Properties, and Microscopic Structure

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