Protein Kinase C Delta Mediates Fibroblast Growth Factor 2-Induced Expression of Interferon Tau in Bovine Trophectoderm.

Yang, Qi; Johnson, Sally; Ealy, Alan D.

doi:10.1093/biolreprod/81.s1.179

Cited by 6 publications

(8 citation statements)

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“…31 Due to the lack of a specific antibody to identify phosphorylated BAG3, the current study could not determine which form of BAG3 was implicated in the EMT of HK2 cells mediated by FGF-2. As numerous functions of FGF-2 have been ascribed to PKCd activation, [40][41][42][43] we thus speculated that, similarly with thyroid cancer cells, a phosphorylated form of BAG3 might be responsible for the EMT of HK2 induced by FGF-2. The generation of an antibody to specifically recognize phosphorylated BAG3 ....................................................................................................................... might be helpful to clarify this issue in the future.…”

Section: Discussionmentioning

confidence: 92%

Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial–mesenchymal transition in renal epithelial cells

Zhang

et al. 2014

Exp Biol Med (Maywood)

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The epithelial-mesenchymal transition (EMT) of tubular epithelial cells to myofibroblast-like cells plays a substantial role in renal tubulointerstitial fibrosis, which is a common pathological character of end-stage renal disease (ESRD). Fibroblast growth factor-2 (FGF-2) triggers EMT in tubular epithelial cells and increases Bcl-2-associated athanogene 3 (BAG3) expression in neural progenitor and neuroblastoma cells. In addition, a novel role of regulation of EMT has been ascribed to BAG3 recently. These previous reports urged us to study the potential involvement of BAG3 in EMT triggered by FGF-2 in renal tubular epithelial cells. The current study found that FGF-2 induced EMT, simultaneously increased BAG3 expression in human kidney 2 (HK2) cells. Although FGF-2 induced EMT in nontransfected or scramble short hairpin RNA (shRNA) transfected HK2 cells, it was ineffective in BAG3-silenced cells, indicating a favorable role of BAG3 in EMT of tubular cells induced by FGF-2. Knockdown of BAG3 also significantly suppressed motion and invasion of HK2 cells mediated by FGF-2. Furthermore, we confirmed that BAG3 was upregulated in kidney of unilateral ureteral obstruction (UUO) rats, a well-established renal fibrosis model, in which EMT is supposed to exert a substantial influence on renal fibrosis. Importantly, upregulation of BAG3 was limited to tubular epithelial cells. Results of the current study identify BAG3 as a potential player in EMT of tubular epithelial cells, as well as renal fibrosis.

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Section: Discussionmentioning

confidence: 92%

Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial–mesenchymal transition in renal epithelial cells

Zhang

et al. 2014

Exp Biol Med (Maywood)

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“…Possible mechanisms include stimulation of cell multiplication and the formation of the blastocele, inhibition of apoptosis, stimulation of interferon-tau production, and reduced interferon-gamma production [4,6,[11][12][13][14]. The GFs and CYKs may act in synergy on the same cellular function and may have identical effects on embryo development [31].…”

Section: Discussionmentioning

confidence: 99%

“…Insulinlike growth factor II decreases the apoptosis index in mouse embryos [4]. Fibroblast growth factor enhances the development of bovine embryos produced in vitro [8], promotes migratory activity in ovine trophoblast cells [9], and increases the production of interferon-tau in bovine trophectoderm (TE), an essential component for the establishment and maintenance of pregnancy in cattle and sheep [10,11]. Leukemia inhibitory factor increases the number of ICM cells in bovine embryos produced in vitro [12].…”

Section: Introductionmentioning

confidence: 99%

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

et al. 2015

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a b s t r a c tThe aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-b1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the best combination of GFs-CYKs þ RA þ HA for optimal embryonic development. Next, we compared development rates and embryo quality (inner cell mass [ICM]-to-total cell number [TCN] ratio), and postthaw survival and hatching rates using this synthetic medium (T1) and a control medium: synthetic oviduct fluid þ BSA þ ITS (insulin, transferrin, and selenium). The blastocyst rates were significantly higher with T1 than those with the control at 7 and 8 days after fertilization. There was no significant difference in TCN or the ICM/TCN ratio between the two treatments. Survival and hatching rates 48 hours after thawing were similar for both treatments. Finally, nine embryo transfers were conducted using fresh and previously frozen Day-7 blastocysts to evaluate the in vivo viability of embryos produced in this synthetic medium; four gestations were obtained from six fresh embryos and one gestation from three frozen embryos. In conclusion, the fetal calf serum and BSA-free medium, supplemented with GFs-CYKs þ RA þ HA, improved embryo development and gave comparable ICM/TCN ratios and postthaw survival rates to the control with BSA. Fresh and frozen embryos produced in this medium are viable for embryo transfer. This fully synthetic method of embryo culture is a useful means of reducing the risk of disease transmission via embryo transfer.

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“…Amount of biologically active IFNT in uterine flushings was determined by measuring the inhibition of vesicular stomatitis virus induced lysis of MadinDarby bovine kidney cells as described elsewhere [41]. Antiviral activity was converted to units of IFN (IU/ml) by comparing inhibition to that achieved with a human IFNA standard (Millipore).…”

Section: Ifnt Assaymentioning

confidence: 99%

Sexual Dimorphism in Developmental Programming of the Bovine Preimplantation Embryo Caused by Colony-Stimulating Factor 21

Dobbs

Gagné

Fournier

et al. 2014

Biology of Reproduction

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Physiology of the adult can be modified by alterations in prenatal development driven by the maternal environment. Developmental programming, which can be established before the embryo implants in the uterus, can affect females differently than males. The mechanism by which sex-specific developmental programming is established is not known. Here we present evidence that maternal regulatory signals change female embryos differently than male embryos. In particular, actions of the maternally derived cytokine CSF2 from Day 5 to Day 7 of development affected characteristics of the embryo at Day 15 differently for females than males. CSF2 decreased length and IFNT secretion of female embryos but increased length and IFNT secretion of male embryos. Analysis of a limited number of samples indicated that changes in the transcriptome and methylome caused by CSF2 also differed between female and males. Thus, sex-specific programming by the maternal environment could occur when changes in secretion of maternally derived regulatory molecules alter development of female embryos differently than male embryos.

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Protein Kinase C Delta Mediates Fibroblast Growth Factor 2-Induced Expression of Interferon Tau in Bovine Trophectoderm.

Cited by 6 publications

References 0 publications

Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial–mesenchymal transition in renal epithelial cells

Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial–mesenchymal transition in renal epithelial cells

In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy

Sexual Dimorphism in Developmental Programming of the Bovine Preimplantation Embryo Caused by Colony-Stimulating Factor 21

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